Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF801 and GFF802 are separated by 125 nucleotides GFF802 and GFF803 are separated by 14 nucleotides GFF803 and GFF804 are separated by 17 nucleotides GFF804 and GFF805 are separated by 116 nucleotides
GFF801 - Murein hydrolase activator NlpD, at 106,988 to 107,503
GFF801
GFF802 - Conserved uncharacterized protein CreA, at 107,629 to 108,117
GFF802
GFF803 - MutT/Nudix family protein, at 108,132 to 108,683
GFF803
GFF804 - Fumarylacetoacetate hydrolase family protein, at 108,701 to 109,402
GFF804
GFF805 - hypothetical protein, at 109,519 to 110,433
GFF805
Position (kb)
108
109 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 107.164 kb on - strand, within GFF801 at 107.164 kb on - strand, within GFF801 at 107.211 kb on + strand, within GFF801 at 107.212 kb on - strand, within GFF801 at 107.212 kb on - strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.235 kb on + strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.236 kb on - strand, within GFF801 at 107.590 kb on + strand at 107.590 kb on + strand at 107.590 kb on + strand at 107.591 kb on - strand at 107.591 kb on - strand at 107.591 kb on - strand at 107.630 kb on + strand at 107.630 kb on + strand at 107.631 kb on - strand at 107.631 kb on - strand at 107.631 kb on - strand at 107.631 kb on - strand at 107.714 kb on + strand, within GFF802 at 107.714 kb on + strand, within GFF802 at 107.714 kb on + strand, within GFF802 at 107.715 kb on - strand, within GFF802 at 107.715 kb on - strand, within GFF802 at 107.715 kb on - strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.723 kb on + strand, within GFF802 at 107.724 kb on - strand, within GFF802 at 107.724 kb on - strand, within GFF802 at 107.724 kb on - strand, within GFF802 at 107.724 kb on - strand, within GFF802 at 107.801 kb on + strand, within GFF802 at 107.801 kb on + strand, within GFF802 at 107.801 kb on + strand, within GFF802 at 107.801 kb on + strand, within GFF802 at 107.801 kb on + strand, within GFF802 at 107.802 kb on - strand, within GFF802 at 107.802 kb on - strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.936 kb on + strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.937 kb on - strand, within GFF802 at 107.969 kb on + strand, within GFF802 at 107.969 kb on + strand, within GFF802 at 107.969 kb on + strand, within GFF802 at 107.969 kb on + strand, within GFF802 at 107.970 kb on - strand, within GFF802 at 107.970 kb on - strand, within GFF802 at 107.970 kb on - strand, within GFF802 at 107.977 kb on + strand, within GFF802 at 107.977 kb on + strand, within GFF802 at 107.977 kb on + strand, within GFF802 at 107.978 kb on - strand, within GFF802 at 107.978 kb on - strand, within GFF802 at 107.978 kb on - strand, within GFF802 at 107.978 kb on - strand, within GFF802 at 108.190 kb on + strand, within GFF803 at 108.190 kb on + strand, within GFF803 at 108.190 kb on + strand, within GFF803 at 108.190 kb on + strand, within GFF803 at 108.191 kb on - strand, within GFF803 at 108.191 kb on - strand, within GFF803 at 108.191 kb on - strand, within GFF803 at 108.319 kb on + strand, within GFF803 at 108.319 kb on + strand, within GFF803 at 108.319 kb on + strand, within GFF803 at 108.562 kb on + strand, within GFF803 at 108.562 kb on + strand, within GFF803 at 108.562 kb on + strand, within GFF803 at 108.562 kb on + strand, within GFF803 at 108.563 kb on - strand, within GFF803 at 108.628 kb on + strand at 108.628 kb on + strand at 108.628 kb on + strand at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.080 kb on + strand, within GFF804 at 109.182 kb on + strand, within GFF804 at 109.246 kb on - strand, within GFF804 at 109.296 kb on + strand, within GFF804 at 109.296 kb on + strand, within GFF804 at 109.296 kb on + strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.297 kb on - strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.314 kb on + strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.315 kb on - strand, within GFF804 at 109.618 kb on + strand, within GFF805 at 109.618 kb on + strand, within GFF805 at 109.619 kb on - strand, within GFF805 at 109.619 kb on - strand, within GFF805 at 109.663 kb on + strand, within GFF805 at 109.663 kb on + strand, within GFF805 at 109.663 kb on + strand, within GFF805 at 109.663 kb on + strand, within GFF805 at 109.664 kb on - strand, within GFF805
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 107,164 - GFF801 0.34 +0.1 107,164 - GFF801 0.34 -0.6 107,211 + GFF801 0.43 -0.0 107,212 - GFF801 0.43 -0.9 107,212 - GFF801 0.43 -0.5 107,235 + GFF801 0.48 +1.6 107,235 + GFF801 0.48 -0.5 107,235 + GFF801 0.48 -0.7 107,235 + GFF801 0.48 -0.3 107,235 + GFF801 0.48 -0.1 107,235 + GFF801 0.48 -1.1 107,235 + GFF801 0.48 -0.1 107,235 + GFF801 0.48 -0.0 107,235 + GFF801 0.48 +0.3 107,235 + GFF801 0.48 -1.9 107,235 + GFF801 0.48 -0.3 107,235 + GFF801 0.48 -0.4 107,236 - GFF801 0.48 -0.8 107,236 - GFF801 0.48 -0.5 107,236 - GFF801 0.48 -0.1 107,236 - GFF801 0.48 +1.0 107,236 - GFF801 0.48 -1.1 107,236 - GFF801 0.48 -1.0 107,236 - GFF801 0.48 -1.4 107,236 - GFF801 0.48 +0.2 107,236 - GFF801 0.48 -1.9 107,590 + +0.3 107,590 + -0.4 107,590 + -0.9 107,591 - +0.4 107,591 - -0.7 107,591 - +0.3 107,630 + -0.3 107,630 + -0.2 107,631 - +0.2 107,631 - -0.7 107,631 - -1.4 107,631 - +0.4 107,714 + GFF802 0.17 +0.4 107,714 + GFF802 0.17 -0.1 107,714 + GFF802 0.17 -0.7 107,715 - GFF802 0.18 -0.9 107,715 - GFF802 0.18 +0.1 107,715 - GFF802 0.18 -0.2 107,723 + GFF802 0.19 +0.2 107,723 + GFF802 0.19 +0.9 107,723 + GFF802 0.19 -0.2 107,723 + GFF802 0.19 -0.3 107,723 + GFF802 0.19 -0.8 107,723 + GFF802 0.19 +0.3 107,724 - GFF802 0.19 -1.3 107,724 - GFF802 0.19 -0.9 107,724 - GFF802 0.19 +0.1 107,724 - GFF802 0.19 +0.1 107,801 + GFF802 0.35 +1.0 107,801 + GFF802 0.35 +0.0 107,801 + GFF802 0.35 -0.8 107,801 + GFF802 0.35 -0.0 107,801 + GFF802 0.35 -1.1 107,802 - GFF802 0.35 -1.5 107,802 - GFF802 0.35 +0.2 107,936 + GFF802 0.63 -1.0 107,936 + GFF802 0.63 +0.3 107,936 + GFF802 0.63 +0.4 107,936 + GFF802 0.63 -0.4 107,936 + GFF802 0.63 -0.8 107,936 + GFF802 0.63 -0.1 107,936 + GFF802 0.63 +0.9 107,936 + GFF802 0.63 -0.7 107,936 + GFF802 0.63 -0.2 107,936 + GFF802 0.63 -1.3 107,936 + GFF802 0.63 -0.8 107,936 + GFF802 0.63 -1.0 107,936 + GFF802 0.63 +0.3 107,936 + GFF802 0.63 -0.3 107,936 + GFF802 0.63 -0.5 107,937 - GFF802 0.63 -1.7 107,937 - GFF802 0.63 -1.9 107,937 - GFF802 0.63 -0.5 107,937 - GFF802 0.63 -0.8 107,937 - GFF802 0.63 -0.2 107,937 - GFF802 0.63 -1.1 107,937 - GFF802 0.63 +0.6 107,937 - GFF802 0.63 -1.6 107,937 - GFF802 0.63 +0.2 107,937 - GFF802 0.63 +0.1 107,937 - GFF802 0.63 -1.8 107,937 - GFF802 0.63 +0.6 107,937 - GFF802 0.63 -0.6 107,937 - GFF802 0.63 +1.6 107,937 - GFF802 0.63 -0.4 107,937 - GFF802 0.63 -0.2 107,937 - GFF802 0.63 -0.7 107,969 + GFF802 0.70 +0.9 107,969 + GFF802 0.70 -1.2 107,969 + GFF802 0.70 -0.2 107,969 + GFF802 0.70 +1.6 107,970 - GFF802 0.70 +0.2 107,970 - GFF802 0.70 -0.5 107,970 - GFF802 0.70 +0.1 107,977 + GFF802 0.71 -0.2 107,977 + GFF802 0.71 +2.0 107,977 + GFF802 0.71 -0.4 107,978 - GFF802 0.71 +0.0 107,978 - GFF802 0.71 -0.8 107,978 - GFF802 0.71 -0.4 107,978 - GFF802 0.71 +0.3 108,190 + GFF803 0.11 +0.6 108,190 + GFF803 0.11 -0.6 108,190 + GFF803 0.11 +0.1 108,190 + GFF803 0.11 +0.6 108,191 - GFF803 0.11 -1.0 108,191 - GFF803 0.11 -1.3 108,191 - GFF803 0.11 -1.2 108,319 + GFF803 0.34 +0.3 108,319 + GFF803 0.34 -0.1 108,319 + GFF803 0.34 -1.1 108,562 + GFF803 0.78 -0.1 108,562 + GFF803 0.78 -1.8 108,562 + GFF803 0.78 +0.2 108,562 + GFF803 0.78 +0.5 108,563 - GFF803 0.78 -1.1 108,628 + -0.1 108,628 + -0.1 108,628 + +1.0 109,080 + GFF804 0.54 +0.8 109,080 + GFF804 0.54 -2.4 109,080 + GFF804 0.54 -1.2 109,080 + GFF804 0.54 +0.1 109,080 + GFF804 0.54 -0.0 109,080 + GFF804 0.54 -0.0 109,080 + GFF804 0.54 -0.8 109,080 + GFF804 0.54 +1.1 109,080 + GFF804 0.54 -1.2 109,080 + GFF804 0.54 -1.1 109,080 + GFF804 0.54 -0.0 109,080 + GFF804 0.54 -0.2 109,080 + GFF804 0.54 -0.9 109,080 + GFF804 0.54 -0.5 109,182 + GFF804 0.69 -0.4 109,246 - GFF804 0.78 +0.8 109,296 + GFF804 0.85 -0.4 109,296 + GFF804 0.85 -0.2 109,296 + GFF804 0.85 +2.0 109,297 - GFF804 0.85 +1.1 109,297 - GFF804 0.85 -0.2 109,297 - GFF804 0.85 -2.0 109,297 - GFF804 0.85 -0.1 109,297 - GFF804 0.85 -0.0 109,297 - GFF804 0.85 +0.8 109,297 - GFF804 0.85 -0.0 109,297 - GFF804 0.85 -0.7 109,297 - GFF804 0.85 -0.0 109,314 + GFF804 0.87 -0.1 109,314 + GFF804 0.87 -0.3 109,314 + GFF804 0.87 +0.1 109,314 + GFF804 0.87 +1.1 109,314 + GFF804 0.87 +0.3 109,314 + GFF804 0.87 -0.7 109,315 - GFF804 0.87 -0.3 109,315 - GFF804 0.87 -0.9 109,315 - GFF804 0.87 -0.2 109,315 - GFF804 0.87 -0.3 109,315 - GFF804 0.87 +0.1 109,315 - GFF804 0.87 +0.4 109,315 - GFF804 0.87 -0.0 109,618 + GFF805 0.11 -0.3 109,618 + GFF805 0.11 +0.2 109,619 - GFF805 0.11 -0.2 109,619 - GFF805 0.11 -0.2 109,663 + GFF805 0.16 -0.6 109,663 + GFF805 0.16 +0.4 109,663 + GFF805 0.16 -0.1 109,663 + GFF805 0.16 -0.2 109,664 - GFF805 0.16 +0.1
Or see this region's nucleotide sequence