Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7306 and GFF7307 are separated by 11 nucleotides GFF7307 and GFF7308 are separated by 15 nucleotides GFF7308 and GFF7309 are separated by 99 nucleotides
GFF7306 - FolM Alternative dihydrofolate reductase 1, at 1,237 to 2,013
GFF7306
GFF7307 - Dihydroneopterin aldolase (EC 4.1.2.25), at 2,025 to 2,423
GFF7307
GFF7308 - Uncharacterized MFS-type transporter, at 2,439 to 3,662
GFF7308
GFF7309 - Transcriptional regulator, LysR family, at 3,762 to 4,712
GFF7309
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.561 kb on + strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.562 kb on - strand, within GFF7306 at 1.593 kb on + strand, within GFF7306 at 1.593 kb on + strand, within GFF7306 at 1.594 kb on - strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.667 kb on + strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.668 kb on - strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.717 kb on + strand, within GFF7306 at 1.718 kb on - strand, within GFF7306 at 1.718 kb on - strand, within GFF7306 at 1.718 kb on - strand, within GFF7306 at 1.718 kb on - strand, within GFF7306 at 2.422 kb on - strand at 2.422 kb on - strand at 2.422 kb on - strand at 2.422 kb on - strand at 2.422 kb on - strand at 2.659 kb on + strand, within GFF7308 at 2.659 kb on + strand, within GFF7308 at 2.660 kb on - strand, within GFF7308 at 3.067 kb on + strand, within GFF7308 at 3.068 kb on - strand, within GFF7308 at 3.399 kb on + strand, within GFF7308 at 3.400 kb on - strand, within GFF7308 at 3.400 kb on - strand, within GFF7308 at 3.478 kb on + strand, within GFF7308 at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.569 kb on - strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.571 kb on + strand at 3.572 kb on - strand at 3.572 kb on - strand at 3.572 kb on - strand at 3.572 kb on - strand at 3.572 kb on - strand at 3.572 kb on - strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.665 kb on + strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.666 kb on - strand at 3.668 kb on + strand at 3.668 kb on + strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.669 kb on - strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.724 kb on + strand at 3.725 kb on - strand at 3.725 kb on - strand at 3.725 kb on - strand at 3.725 kb on - strand at 3.727 kb on + strand at 3.727 kb on + strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.728 kb on - strand at 3.749 kb on - strand at 3.750 kb on + strand at 3.750 kb on + strand at 3.750 kb on + strand at 3.751 kb on - strand at 3.751 kb on - strand at 3.751 kb on - strand at 3.762 kb on - strand at 4.149 kb on + strand, within GFF7309 at 4.149 kb on + strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.150 kb on - strand, within GFF7309 at 4.161 kb on + strand, within GFF7309 at 4.162 kb on - strand, within GFF7309 at 4.407 kb on + strand, within GFF7309 at 4.407 kb on + strand, within GFF7309 at 4.408 kb on - strand, within GFF7309 at 4.408 kb on - strand, within GFF7309 at 4.408 kb on - strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.452 kb on + strand, within GFF7309 at 4.453 kb on - strand, within GFF7309 at 4.453 kb on - strand, within GFF7309 at 4.453 kb on - strand, within GFF7309 at 4.453 kb on - strand, within GFF7309 at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.626 kb on + strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.627 kb on - strand at 4.635 kb on + strand at 4.635 kb on + strand at 4.635 kb on + strand at 4.635 kb on + strand at 4.635 kb on + strand at 4.636 kb on - strand at 4.636 kb on - strand at 4.636 kb on - strand at 4.636 kb on - strand at 4.636 kb on - strand at 4.641 kb on + strand at 4.641 kb on + strand at 4.642 kb on - strand at 4.653 kb on + strand at 4.654 kb on - strand at 4.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 1,561 + GFF7306 0.42 +0.8 1,561 + GFF7306 0.42 +0.3 1,561 + GFF7306 0.42 -1.2 1,561 + GFF7306 0.42 +1.0 1,561 + GFF7306 0.42 -0.3 1,561 + GFF7306 0.42 +0.6 1,561 + GFF7306 0.42 -1.1 1,561 + GFF7306 0.42 +1.6 1,562 - GFF7306 0.42 +1.8 1,562 - GFF7306 0.42 +0.3 1,562 - GFF7306 0.42 +0.5 1,562 - GFF7306 0.42 -0.3 1,562 - GFF7306 0.42 +0.4 1,562 - GFF7306 0.42 +1.5 1,562 - GFF7306 0.42 +0.8 1,562 - GFF7306 0.42 +0.2 1,562 - GFF7306 0.42 +0.4 1,562 - GFF7306 0.42 +0.8 1,562 - GFF7306 0.42 +0.3 1,562 - GFF7306 0.42 +0.2 1,593 + GFF7306 0.46 +1.9 1,593 + GFF7306 0.46 +0.5 1,594 - GFF7306 0.46 +0.3 1,667 + GFF7306 0.55 +0.7 1,667 + GFF7306 0.55 +1.2 1,667 + GFF7306 0.55 +0.6 1,667 + GFF7306 0.55 -0.1 1,667 + GFF7306 0.55 +0.5 1,667 + GFF7306 0.55 +0.2 1,668 - GFF7306 0.55 +1.2 1,668 - GFF7306 0.55 +0.5 1,668 - GFF7306 0.55 +0.7 1,668 - GFF7306 0.55 -0.7 1,668 - GFF7306 0.55 -0.7 1,668 - GFF7306 0.55 +0.1 1,668 - GFF7306 0.55 +1.5 1,668 - GFF7306 0.55 +1.3 1,717 + GFF7306 0.62 -1.5 1,717 + GFF7306 0.62 +0.6 1,717 + GFF7306 0.62 +0.5 1,717 + GFF7306 0.62 +0.6 1,717 + GFF7306 0.62 +1.0 1,717 + GFF7306 0.62 +0.7 1,718 - GFF7306 0.62 +1.4 1,718 - GFF7306 0.62 +0.5 1,718 - GFF7306 0.62 +1.1 1,718 - GFF7306 0.62 +0.7 2,422 - -0.0 2,422 - -0.1 2,422 - -0.4 2,422 - -0.2 2,422 - -0.5 2,659 + GFF7308 0.18 +0.2 2,659 + GFF7308 0.18 +0.5 2,660 - GFF7308 0.18 -0.5 3,067 + GFF7308 0.51 +0.1 3,068 - GFF7308 0.51 -0.6 3,399 + GFF7308 0.78 -0.4 3,400 - GFF7308 0.79 -0.2 3,400 - GFF7308 0.79 +0.1 3,478 + GFF7308 0.85 -0.2 3,568 + +0.1 3,568 + -1.3 3,568 + +0.6 3,568 + +0.6 3,568 + -0.8 3,568 + +0.4 3,568 + +0.5 3,568 + +0.0 3,568 + -0.3 3,569 - +0.3 3,569 - +3.1 3,569 - -0.8 3,569 - -0.5 3,569 - -1.2 3,569 - +0.4 3,569 - -0.1 3,569 - -1.0 3,569 - -0.9 3,569 - -1.0 3,569 - -0.5 3,569 - -0.8 3,571 + +0.5 3,571 + -0.3 3,571 + -1.2 3,571 + -0.8 3,571 + +0.0 3,571 + -0.7 3,571 + -0.1 3,571 + +0.2 3,571 + +0.2 3,572 - -0.5 3,572 - +0.6 3,572 - -0.2 3,572 - +1.0 3,572 - +1.4 3,572 - +0.0 3,665 + -1.0 3,665 + -0.3 3,665 + +1.0 3,665 + -0.3 3,665 + -1.0 3,665 + -1.6 3,665 + +0.0 3,665 + +0.7 3,665 + +0.2 3,666 - +0.0 3,666 - -0.3 3,666 - +0.1 3,666 - -0.5 3,666 - +1.1 3,666 - -1.2 3,666 - +0.3 3,666 - -0.7 3,666 - -0.0 3,668 + +1.4 3,668 + -0.9 3,669 - -0.0 3,669 - +0.1 3,669 - +0.4 3,669 - +0.1 3,669 - -1.0 3,669 - +0.0 3,669 - -1.0 3,724 + +0.0 3,724 + +0.0 3,724 + -0.3 3,724 + -0.2 3,724 + -0.6 3,724 + -0.6 3,724 + -0.2 3,724 + -0.3 3,724 + -1.0 3,725 - -0.1 3,725 - +0.5 3,725 - -1.0 3,725 - -0.5 3,727 + +0.3 3,727 + +0.5 3,728 - -1.4 3,728 - +0.8 3,728 - -1.2 3,728 - -0.5 3,728 - +1.0 3,728 - -0.5 3,728 - +0.7 3,728 - -0.8 3,728 - -0.3 3,749 - -0.3 3,750 + -0.9 3,750 + -0.7 3,750 + -0.0 3,751 - -1.7 3,751 - -0.2 3,751 - -0.7 3,762 - -0.4 4,149 + GFF7309 0.41 -1.8 4,149 + GFF7309 0.41 -0.7 4,150 - GFF7309 0.41 +0.3 4,150 - GFF7309 0.41 +0.5 4,150 - GFF7309 0.41 -1.5 4,150 - GFF7309 0.41 +0.9 4,150 - GFF7309 0.41 +0.8 4,150 - GFF7309 0.41 +0.8 4,161 + GFF7309 0.42 -0.1 4,162 - GFF7309 0.42 -0.3 4,407 + GFF7309 0.68 -0.4 4,407 + GFF7309 0.68 -0.1 4,408 - GFF7309 0.68 -1.7 4,408 - GFF7309 0.68 +0.7 4,408 - GFF7309 0.68 -0.5 4,452 + GFF7309 0.73 -0.1 4,452 + GFF7309 0.73 +1.2 4,452 + GFF7309 0.73 +0.7 4,452 + GFF7309 0.73 -1.1 4,452 + GFF7309 0.73 -0.0 4,452 + GFF7309 0.73 +0.1 4,453 - GFF7309 0.73 -1.0 4,453 - GFF7309 0.73 -1.2 4,453 - GFF7309 0.73 +0.4 4,453 - GFF7309 0.73 +0.0 4,626 + -2.0 4,626 + -1.2 4,626 + -0.0 4,626 + -0.2 4,626 + -0.4 4,626 + +0.1 4,626 + +0.5 4,626 + +0.6 4,626 + -0.7 4,626 + -0.1 4,626 + -0.2 4,626 + +1.1 4,626 + -1.5 4,626 + +0.7 4,626 + -0.5 4,626 + -0.4 4,626 + -1.0 4,626 + -0.5 4,626 + -0.5 4,626 + -1.2 4,626 + +0.8 4,626 + +1.2 4,626 + -0.9 4,626 + -0.0 4,627 - +0.2 4,627 - -0.5 4,627 - -0.3 4,627 - -0.2 4,627 - +0.1 4,627 - +0.9 4,627 - +0.7 4,627 - -0.5 4,627 - +0.2 4,627 - -0.8 4,627 - -0.4 4,627 - -0.0 4,627 - +0.0 4,627 - -0.0 4,627 - +0.5 4,627 - +1.0 4,627 - -0.5 4,627 - -0.7 4,627 - -0.1 4,627 - -0.3 4,627 - -0.7 4,635 + -0.0 4,635 + -1.7 4,635 + -0.4 4,635 + +0.0 4,635 + +0.1 4,636 - +2.4 4,636 - -0.4 4,636 - -0.5 4,636 - +0.7 4,636 - -0.6 4,641 + +1.1 4,641 + -0.1 4,642 - -0.8 4,653 + -0.2 4,654 - +0.3 4,654 - +0.6
Or see this region's nucleotide sequence