Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6442 and GFF6443 are separated by 3 nucleotides GFF6443 and GFF6444 are separated by 51 nucleotides GFF6444 and GFF6445 are separated by 83 nucleotides GFF6445 and GFF6446 overlap by 14 nucleotides
GFF6442 - Aldehyde dehydrogenase (EC 1.2.1.3), at 17,672 to 19,192
GFF6442
GFF6443 - no description, at 19,196 to 19,369
GFF6443
GFF6444 - no description, at 19,421 to 21,316
GFF6444
GFF6445 - Nitroreductase, at 21,400 to 22,308
GFF6445
GFF6446 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 22,295 to 23,368
GFF6446
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.441 kb on + strand, within GFF6442 at 18.653 kb on + strand, within GFF6442 at 18.747 kb on + strand, within GFF6442 at 18.747 kb on + strand, within GFF6442 at 18.748 kb on - strand, within GFF6442 at 18.813 kb on + strand, within GFF6442 at 18.813 kb on + strand, within GFF6442 at 19.089 kb on + strand at 19.089 kb on + strand at 19.090 kb on - strand at 19.090 kb on - strand at 19.090 kb on - strand at 19.090 kb on - strand at 19.090 kb on - strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.128 kb on + strand at 19.129 kb on - strand at 19.129 kb on - strand at 19.129 kb on - strand at 19.137 kb on + strand at 19.138 kb on - strand at 19.262 kb on + strand, within GFF6443 at 19.263 kb on - strand, within GFF6443 at 19.263 kb on - strand, within GFF6443 at 19.291 kb on + strand, within GFF6443 at 19.291 kb on + strand, within GFF6443 at 19.361 kb on - strand at 19.367 kb on + strand at 19.367 kb on + strand at 19.368 kb on - strand at 19.368 kb on - strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.559 kb on + strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.560 kb on - strand at 19.671 kb on + strand, within GFF6444 at 19.671 kb on + strand, within GFF6444 at 19.671 kb on + strand, within GFF6444 at 19.672 kb on - strand, within GFF6444 at 19.729 kb on - strand, within GFF6444 at 19.871 kb on + strand, within GFF6444 at 19.871 kb on + strand, within GFF6444 at 19.872 kb on - strand, within GFF6444 at 19.872 kb on - strand, within GFF6444 at 19.872 kb on - strand, within GFF6444 at 19.872 kb on - strand, within GFF6444 at 20.357 kb on - strand, within GFF6444 at 20.582 kb on + strand, within GFF6444 at 20.582 kb on + strand, within GFF6444 at 20.583 kb on - strand, within GFF6444 at 20.815 kb on - strand, within GFF6444 at 20.846 kb on + strand, within GFF6444 at 20.846 kb on + strand, within GFF6444 at 20.994 kb on - strand, within GFF6444 at 21.344 kb on - strand at 21.357 kb on + strand at 21.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 18,441 + GFF6442 0.51 -1.1 18,653 + GFF6442 0.64 +1.6 18,747 + GFF6442 0.71 +0.2 18,747 + GFF6442 0.71 -1.0 18,748 - GFF6442 0.71 +0.9 18,813 + GFF6442 0.75 +0.9 18,813 + GFF6442 0.75 +0.0 19,089 + +0.0 19,089 + -0.1 19,090 - +1.0 19,090 - -0.1 19,090 - -0.7 19,090 - -0.5 19,090 - -2.5 19,128 + +0.6 19,128 + -0.4 19,128 + +0.0 19,128 + -0.8 19,128 + -0.6 19,128 + -0.2 19,128 + +0.3 19,128 + +0.9 19,129 - +0.2 19,129 - -0.1 19,129 - +1.2 19,137 + +0.3 19,138 - +1.8 19,262 + GFF6443 0.38 -0.7 19,263 - GFF6443 0.39 -1.1 19,263 - GFF6443 0.39 -0.6 19,291 + GFF6443 0.55 -0.4 19,291 + GFF6443 0.55 -0.3 19,361 - +0.3 19,367 + +1.0 19,367 + -0.4 19,368 - -0.4 19,368 - -1.4 19,559 + +0.6 19,559 + -1.2 19,559 + -1.4 19,559 + -0.2 19,559 + +0.2 19,559 + -0.4 19,559 + -0.8 19,560 - -1.0 19,560 - +0.3 19,560 - +0.1 19,560 - +0.4 19,560 - -0.5 19,560 - -2.3 19,560 - +0.5 19,560 - -0.2 19,560 - -0.0 19,560 - -0.4 19,671 + GFF6444 0.13 -0.5 19,671 + GFF6444 0.13 -0.3 19,671 + GFF6444 0.13 +0.0 19,672 - GFF6444 0.13 +1.2 19,729 - GFF6444 0.16 -0.7 19,871 + GFF6444 0.24 +1.6 19,871 + GFF6444 0.24 -0.5 19,872 - GFF6444 0.24 -0.6 19,872 - GFF6444 0.24 -1.7 19,872 - GFF6444 0.24 -0.3 19,872 - GFF6444 0.24 -0.0 20,357 - GFF6444 0.49 -1.0 20,582 + GFF6444 0.61 +0.8 20,582 + GFF6444 0.61 -0.5 20,583 - GFF6444 0.61 +0.8 20,815 - GFF6444 0.74 -1.2 20,846 + GFF6444 0.75 -0.3 20,846 + GFF6444 0.75 -0.5 20,994 - GFF6444 0.83 -0.3 21,344 - -0.6 21,357 + -0.8 21,357 + -1.7
Or see this region's nucleotide sequence