Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6270 and GFF6272 are separated by 51 nucleotides GFF6272 and GFF6271 overlap by 2 nucleotides GFF6271 and GFF6273 are separated by 50 nucleotides
GFF6270 - Uncharacterized MFS-type transporter, at 62,195 to 63,646
GFF6270
GFF6272 - no description, at 63,698 to 63,832
GFF6272
GFF6271 - Phosphatidylglycerophosphatase A (EC 3.1.3.27), at 63,831 to 64,358
GFF6271
GFF6273 - Nicotinamide-nucleotide amidase (EC 3.5.1.42), at 64,409 to 64,918
GFF6273
Position (kb) 63
64 Strain fitness (log2 ratio) -1
0
1
2 at 62.736 kb on + strand, within GFF6270 at 62.737 kb on - strand, within GFF6270 at 62.820 kb on + strand, within GFF6270 at 62.820 kb on + strand, within GFF6270 at 62.821 kb on - strand, within GFF6270 at 62.821 kb on - strand, within GFF6270 at 62.821 kb on - strand, within GFF6270 at 63.240 kb on + strand, within GFF6270 at 63.241 kb on - strand, within GFF6270 at 63.241 kb on - strand, within GFF6270 at 63.241 kb on - strand, within GFF6270 at 63.459 kb on + strand, within GFF6270 at 63.459 kb on + strand, within GFF6270 at 63.460 kb on - strand, within GFF6270 at 63.460 kb on - strand, within GFF6270 at 63.685 kb on + strand at 63.685 kb on + strand at 63.685 kb on + strand at 63.686 kb on - strand at 63.686 kb on - strand at 63.686 kb on - strand at 63.686 kb on - strand at 63.686 kb on - strand at 63.686 kb on - strand at 63.703 kb on + strand at 63.703 kb on + strand at 63.704 kb on - strand at 63.712 kb on + strand, within GFF6272 at 63.712 kb on + strand, within GFF6272 at 63.712 kb on + strand, within GFF6272 at 63.713 kb on - strand, within GFF6272 at 63.713 kb on - strand, within GFF6272 at 63.713 kb on - strand, within GFF6272 at 63.713 kb on - strand, within GFF6272 at 63.713 kb on - strand, within GFF6272 at 63.724 kb on - strand, within GFF6272 at 63.724 kb on - strand, within GFF6272 at 63.727 kb on + strand, within GFF6272 at 63.727 kb on + strand, within GFF6272 at 63.727 kb on + strand, within GFF6272 at 63.728 kb on - strand, within GFF6272 at 64.191 kb on + strand, within GFF6271 at 64.192 kb on - strand, within GFF6271 at 64.356 kb on + strand at 64.356 kb on + strand at 64.356 kb on + strand at 64.357 kb on - strand at 64.357 kb on - strand at 64.357 kb on - strand at 64.357 kb on - strand at 64.357 kb on - strand at 64.357 kb on - strand at 64.589 kb on + strand, within GFF6273 at 64.589 kb on + strand, within GFF6273 at 64.589 kb on + strand, within GFF6273 at 64.589 kb on + strand, within GFF6273 at 64.589 kb on + strand, within GFF6273 at 64.589 kb on + strand, within GFF6273 at 64.590 kb on - strand, within GFF6273 at 64.590 kb on - strand, within GFF6273 at 64.590 kb on - strand, within GFF6273 at 64.590 kb on - strand, within GFF6273 at 64.590 kb on - strand, within GFF6273 at 64.590 kb on - strand, within GFF6273
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 62,736 + GFF6270 0.37 +0.3 62,737 - GFF6270 0.37 +0.1 62,820 + GFF6270 0.43 -1.4 62,820 + GFF6270 0.43 -0.9 62,821 - GFF6270 0.43 +0.7 62,821 - GFF6270 0.43 +0.9 62,821 - GFF6270 0.43 -0.2 63,240 + GFF6270 0.72 -0.2 63,241 - GFF6270 0.72 -1.1 63,241 - GFF6270 0.72 -0.0 63,241 - GFF6270 0.72 -1.0 63,459 + GFF6270 0.87 -0.6 63,459 + GFF6270 0.87 +0.1 63,460 - GFF6270 0.87 -0.4 63,460 - GFF6270 0.87 -0.2 63,685 + +0.3 63,685 + +0.7 63,685 + +0.9 63,686 - +0.6 63,686 - +1.1 63,686 - +0.5 63,686 - +0.5 63,686 - +0.2 63,686 - +0.2 63,703 + -0.7 63,703 + +0.3 63,704 - +0.1 63,712 + GFF6272 0.10 +0.3 63,712 + GFF6272 0.10 +0.6 63,712 + GFF6272 0.10 +0.2 63,713 - GFF6272 0.11 +1.6 63,713 - GFF6272 0.11 -0.3 63,713 - GFF6272 0.11 +0.6 63,713 - GFF6272 0.11 +0.1 63,713 - GFF6272 0.11 +1.5 63,724 - GFF6272 0.19 +0.0 63,724 - GFF6272 0.19 -0.4 63,727 + GFF6272 0.21 -0.7 63,727 + GFF6272 0.21 -0.2 63,727 + GFF6272 0.21 +0.1 63,728 - GFF6272 0.22 +0.5 64,191 + GFF6271 0.68 -0.4 64,192 - GFF6271 0.68 -0.4 64,356 + +0.1 64,356 + +1.3 64,356 + -1.2 64,357 - +0.3 64,357 - -1.2 64,357 - -1.5 64,357 - +1.9 64,357 - +0.6 64,357 - -0.5 64,589 + GFF6273 0.35 -0.5 64,589 + GFF6273 0.35 -1.3 64,589 + GFF6273 0.35 -0.2 64,589 + GFF6273 0.35 -0.5 64,589 + GFF6273 0.35 -0.2 64,589 + GFF6273 0.35 -0.5 64,590 - GFF6273 0.35 -1.2 64,590 - GFF6273 0.35 +1.2 64,590 - GFF6273 0.35 -0.9 64,590 - GFF6273 0.35 -0.5 64,590 - GFF6273 0.35 +0.1 64,590 - GFF6273 0.35 -0.8
Or see this region's nucleotide sequence