Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6232 and GFF6233 are separated by 2 nucleotides GFF6233 and GFF6234 are separated by 1 nucleotides GFF6234 and GFF6235 overlap by 4 nucleotides
GFF6232 - hypothetical protein, at 16,283 to 21,721
GFF6232
GFF6233 - hypothetical protein, at 21,724 to 23,433
GFF6233
GFF6234 - hypothetical protein, at 23,435 to 23,584
GFF6234
GFF6235 - hypothetical protein, at 23,581 to 25,701
GFF6235
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -2
-1
0
1
2 at 21.750 kb on - strand at 21.899 kb on + strand, within GFF6233 at 21.900 kb on - strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.454 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.586 kb on + strand, within GFF6233 at 22.587 kb on - strand, within GFF6233 at 22.698 kb on - strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.722 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.133 kb on - strand, within GFF6233 at 23.407 kb on - strand at 23.483 kb on - strand, within GFF6234 at 23.601 kb on + strand at 23.601 kb on + strand at 23.602 kb on - strand at 23.704 kb on + strand at 23.704 kb on + strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.705 kb on - strand at 23.984 kb on + strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 23.985 kb on - strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.089 kb on + strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.090 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.097 kb on - strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.155 kb on + strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.156 kb on - strand, within GFF6235 at 24.282 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235 at 24.318 kb on - strand, within GFF6235
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 21,750 - +0.5 21,899 + GFF6233 0.10 +0.5 21,900 - GFF6233 0.10 -0.2 22,121 + GFF6233 0.23 -0.0 22,121 + GFF6233 0.23 +0.7 22,122 - GFF6233 0.23 +0.1 22,122 - GFF6233 0.23 -0.7 22,454 + GFF6233 0.43 +0.3 22,526 + GFF6233 0.47 -0.4 22,526 + GFF6233 0.47 -0.0 22,586 + GFF6233 0.50 +0.7 22,587 - GFF6233 0.50 +0.3 22,698 - GFF6233 0.57 +0.7 22,721 + GFF6233 0.58 +1.1 22,721 + GFF6233 0.58 +0.9 22,722 - GFF6233 0.58 -0.2 23,133 - GFF6233 0.82 +1.3 23,133 - GFF6233 0.82 +0.1 23,407 - +2.3 23,483 - GFF6234 0.32 -0.1 23,601 + +0.3 23,601 + +1.3 23,602 - +0.0 23,704 + +0.5 23,704 + -2.1 23,705 - -1.2 23,705 - +0.2 23,705 - -0.4 23,705 - -0.6 23,705 - -0.5 23,984 + GFF6235 0.19 +0.6 23,985 - GFF6235 0.19 +0.6 23,985 - GFF6235 0.19 +0.2 24,089 + GFF6235 0.24 +0.2 24,089 + GFF6235 0.24 +0.5 24,089 + GFF6235 0.24 -0.2 24,089 + GFF6235 0.24 -0.1 24,089 + GFF6235 0.24 +0.8 24,089 + GFF6235 0.24 -0.7 24,089 + GFF6235 0.24 +0.4 24,089 + GFF6235 0.24 -0.1 24,090 - GFF6235 0.24 -0.3 24,090 - GFF6235 0.24 +0.4 24,090 - GFF6235 0.24 +0.9 24,090 - GFF6235 0.24 -0.3 24,090 - GFF6235 0.24 +0.1 24,097 - GFF6235 0.24 +0.9 24,097 - GFF6235 0.24 +0.3 24,155 + GFF6235 0.27 -0.1 24,155 + GFF6235 0.27 +0.3 24,156 - GFF6235 0.27 -0.6 24,156 - GFF6235 0.27 -1.2 24,156 - GFF6235 0.27 +0.3 24,282 - GFF6235 0.33 -1.3 24,318 - GFF6235 0.35 +1.3 24,318 - GFF6235 0.35 -0.8 24,318 - GFF6235 0.35 -0.5
Or see this region's nucleotide sequence