Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF586 and GFF587 are separated by 32 nucleotides GFF587 and GFF588 are separated by 85 nucleotides GFF588 and GFF589 are separated by 70 nucleotides
GFF586 - Pirin, at 633,610 to 634,533
GFF586
GFF587 - Multimeric flavodoxin WrbA, at 634,566 to 635,117
GFF587
GFF588 - putative membrane protein, at 635,203 to 635,622
GFF588
GFF589 - Pirin, at 635,693 to 636,394
GFF589
Position (kb)
634
635
636 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.576 kb on + strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.577 kb on - strand at 633.630 kb on + strand at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.776 kb on + strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.777 kb on - strand, within GFF586 at 633.926 kb on + strand, within GFF586 at 633.926 kb on + strand, within GFF586 at 633.927 kb on - strand, within GFF586 at 633.927 kb on - strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.995 kb on + strand, within GFF586 at 633.996 kb on - strand, within GFF586 at 634.181 kb on + strand, within GFF586 at 634.181 kb on + strand, within GFF586 at 634.181 kb on + strand, within GFF586 at 634.181 kb on + strand, within GFF586 at 634.182 kb on - strand, within GFF586 at 634.182 kb on - strand, within GFF586 at 634.182 kb on - strand, within GFF586 at 634.182 kb on - strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.322 kb on + strand, within GFF586 at 634.323 kb on - strand, within GFF586 at 634.323 kb on - strand, within GFF586 at 634.323 kb on - strand, within GFF586 at 634.323 kb on - strand, within GFF586 at 634.323 kb on - strand, within GFF586 at 634.567 kb on + strand at 634.568 kb on - strand at 634.688 kb on - strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.699 kb on + strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.700 kb on - strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.795 kb on + strand, within GFF587 at 634.796 kb on - strand, within GFF587 at 634.796 kb on - strand, within GFF587 at 634.796 kb on - strand, within GFF587 at 634.796 kb on - strand, within GFF587 at 634.796 kb on - strand, within GFF587 at 634.879 kb on + strand, within GFF587 at 634.880 kb on - strand, within GFF587 at 634.880 kb on - strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.939 kb on + strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.940 kb on - strand, within GFF587 at 634.953 kb on + strand, within GFF587 at 634.953 kb on + strand, within GFF587 at 634.953 kb on + strand, within GFF587 at 634.954 kb on - strand, within GFF587 at 634.954 kb on - strand, within GFF587 at 634.954 kb on - strand, within GFF587 at 634.954 kb on - strand, within GFF587 at 635.122 kb on + strand at 635.122 kb on + strand at 635.122 kb on + strand at 635.122 kb on + strand at 635.122 kb on + strand at 635.123 kb on - strand at 635.133 kb on + strand at 635.133 kb on + strand at 635.134 kb on - strand at 635.134 kb on - strand at 635.134 kb on - strand at 635.204 kb on + strand at 635.205 kb on - strand at 635.205 kb on - strand at 635.211 kb on + strand at 635.330 kb on + strand, within GFF588 at 635.330 kb on + strand, within GFF588 at 635.330 kb on + strand, within GFF588 at 635.330 kb on + strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.331 kb on - strand, within GFF588 at 635.407 kb on + strand, within GFF588 at 635.407 kb on + strand, within GFF588 at 635.407 kb on + strand, within GFF588 at 635.408 kb on - strand, within GFF588 at 635.492 kb on + strand, within GFF588 at 635.492 kb on + strand, within GFF588 at 635.493 kb on - strand, within GFF588 at 635.493 kb on - strand, within GFF588 at 635.493 kb on - strand, within GFF588 at 635.549 kb on + strand, within GFF588 at 635.549 kb on + strand, within GFF588 at 635.549 kb on + strand, within GFF588 at 635.549 kb on + strand, within GFF588 at 635.549 kb on + strand, within GFF588 at 635.550 kb on - strand, within GFF588 at 635.550 kb on - strand, within GFF588 at 635.550 kb on - strand, within GFF588 at 635.550 kb on - strand, within GFF588 at 635.656 kb on - strand at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.835 kb on + strand, within GFF589 at 635.836 kb on - strand, within GFF589 at 635.836 kb on - strand, within GFF589 at 635.836 kb on - strand, within GFF589 at 635.836 kb on - strand, within GFF589 at 635.836 kb on - strand, within GFF589 at 635.898 kb on + strand, within GFF589 at 635.899 kb on - strand, within GFF589 at 635.899 kb on - strand, within GFF589 at 635.899 kb on - strand, within GFF589 at 636.007 kb on - strand, within GFF589
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 633,576 + -0.3 633,576 + +1.0 633,576 + +0.4 633,576 + -0.4 633,576 + -2.0 633,576 + -0.8 633,576 + -0.6 633,576 + -1.4 633,576 + +0.9 633,576 + +1.1 633,576 + -1.0 633,576 + +0.2 633,576 + -1.2 633,577 - +0.4 633,577 - +0.0 633,577 - +0.2 633,577 - -1.3 633,577 - +1.4 633,577 - +0.5 633,630 + +1.0 633,776 + GFF586 0.18 -2.3 633,776 + GFF586 0.18 -0.3 633,776 + GFF586 0.18 -1.2 633,776 + GFF586 0.18 -2.1 633,776 + GFF586 0.18 -0.3 633,777 - GFF586 0.18 -0.0 633,777 - GFF586 0.18 -1.7 633,777 - GFF586 0.18 +1.4 633,777 - GFF586 0.18 +0.6 633,777 - GFF586 0.18 +0.4 633,926 + GFF586 0.34 -0.8 633,926 + GFF586 0.34 -1.4 633,927 - GFF586 0.34 +1.0 633,927 - GFF586 0.34 -0.1 633,995 + GFF586 0.42 +0.3 633,995 + GFF586 0.42 +0.5 633,995 + GFF586 0.42 -0.8 633,996 - GFF586 0.42 -0.7 634,181 + GFF586 0.62 -0.4 634,181 + GFF586 0.62 -0.3 634,181 + GFF586 0.62 -0.8 634,181 + GFF586 0.62 -0.6 634,182 - GFF586 0.62 +0.3 634,182 - GFF586 0.62 -0.1 634,182 - GFF586 0.62 -0.0 634,182 - GFF586 0.62 +0.4 634,322 + GFF586 0.77 -0.4 634,322 + GFF586 0.77 +1.4 634,322 + GFF586 0.77 -0.8 634,322 + GFF586 0.77 +0.8 634,322 + GFF586 0.77 +0.4 634,322 + GFF586 0.77 -1.6 634,322 + GFF586 0.77 -0.4 634,322 + GFF586 0.77 -0.4 634,323 - GFF586 0.77 -0.8 634,323 - GFF586 0.77 -0.6 634,323 - GFF586 0.77 -0.0 634,323 - GFF586 0.77 -0.6 634,323 - GFF586 0.77 -1.3 634,567 + -0.4 634,568 - -1.1 634,688 - GFF587 0.22 -0.1 634,699 + GFF587 0.24 -0.7 634,699 + GFF587 0.24 -2.4 634,699 + GFF587 0.24 -0.2 634,699 + GFF587 0.24 -1.1 634,699 + GFF587 0.24 -0.9 634,699 + GFF587 0.24 +1.6 634,699 + GFF587 0.24 +0.3 634,699 + GFF587 0.24 -0.9 634,700 - GFF587 0.24 -0.0 634,700 - GFF587 0.24 -0.7 634,700 - GFF587 0.24 +2.8 634,700 - GFF587 0.24 -2.3 634,700 - GFF587 0.24 -1.0 634,700 - GFF587 0.24 -1.7 634,700 - GFF587 0.24 -1.2 634,700 - GFF587 0.24 +0.4 634,700 - GFF587 0.24 -0.5 634,700 - GFF587 0.24 +0.4 634,700 - GFF587 0.24 -0.3 634,795 + GFF587 0.41 +1.2 634,795 + GFF587 0.41 -1.0 634,795 + GFF587 0.41 +0.4 634,795 + GFF587 0.41 +0.4 634,795 + GFF587 0.41 -0.6 634,795 + GFF587 0.41 -0.6 634,795 + GFF587 0.41 +2.0 634,796 - GFF587 0.42 -0.4 634,796 - GFF587 0.42 +0.6 634,796 - GFF587 0.42 +0.6 634,796 - GFF587 0.42 -0.6 634,796 - GFF587 0.42 -1.2 634,879 + GFF587 0.57 -0.6 634,880 - GFF587 0.57 +0.2 634,880 - GFF587 0.57 -0.9 634,939 + GFF587 0.68 -1.1 634,939 + GFF587 0.68 -0.3 634,939 + GFF587 0.68 -1.1 634,939 + GFF587 0.68 -0.4 634,939 + GFF587 0.68 -1.8 634,939 + GFF587 0.68 -0.6 634,939 + GFF587 0.68 -2.3 634,939 + GFF587 0.68 -0.7 634,939 + GFF587 0.68 +0.5 634,939 + GFF587 0.68 +0.4 634,939 + GFF587 0.68 +1.8 634,940 - GFF587 0.68 +1.4 634,940 - GFF587 0.68 -1.2 634,940 - GFF587 0.68 +0.0 634,940 - GFF587 0.68 -1.4 634,940 - GFF587 0.68 -0.7 634,940 - GFF587 0.68 -0.5 634,940 - GFF587 0.68 -1.6 634,940 - GFF587 0.68 +0.8 634,940 - GFF587 0.68 -1.6 634,940 - GFF587 0.68 -0.3 634,940 - GFF587 0.68 +1.7 634,940 - GFF587 0.68 -0.7 634,940 - GFF587 0.68 -0.4 634,953 + GFF587 0.70 -1.8 634,953 + GFF587 0.70 -0.8 634,953 + GFF587 0.70 -0.1 634,954 - GFF587 0.70 -1.6 634,954 - GFF587 0.70 +0.0 634,954 - GFF587 0.70 -0.1 634,954 - GFF587 0.70 -0.1 635,122 + -0.0 635,122 + -0.3 635,122 + -2.5 635,122 + +1.4 635,122 + +0.1 635,123 - -0.3 635,133 + -0.5 635,133 + -1.0 635,134 - -1.1 635,134 - -0.0 635,134 - -0.1 635,204 + -0.8 635,205 - -0.1 635,205 - +0.2 635,211 + +0.2 635,330 + GFF588 0.30 +0.3 635,330 + GFF588 0.30 -1.0 635,330 + GFF588 0.30 -1.2 635,330 + GFF588 0.30 -0.7 635,331 - GFF588 0.30 +1.8 635,331 - GFF588 0.30 +0.4 635,331 - GFF588 0.30 +0.5 635,331 - GFF588 0.30 +0.4 635,331 - GFF588 0.30 +0.8 635,331 - GFF588 0.30 -0.6 635,331 - GFF588 0.30 -0.2 635,331 - GFF588 0.30 -0.1 635,331 - GFF588 0.30 +0.6 635,331 - GFF588 0.30 +1.4 635,407 + GFF588 0.49 -1.4 635,407 + GFF588 0.49 -2.2 635,407 + GFF588 0.49 -0.1 635,408 - GFF588 0.49 +1.8 635,492 + GFF588 0.69 -0.9 635,492 + GFF588 0.69 +0.5 635,493 - GFF588 0.69 -0.4 635,493 - GFF588 0.69 -2.2 635,493 - GFF588 0.69 -0.4 635,549 + GFF588 0.82 -0.7 635,549 + GFF588 0.82 -1.3 635,549 + GFF588 0.82 -0.6 635,549 + GFF588 0.82 +1.0 635,549 + GFF588 0.82 -2.0 635,550 - GFF588 0.83 -0.8 635,550 - GFF588 0.83 +0.1 635,550 - GFF588 0.83 +1.7 635,550 - GFF588 0.83 -0.5 635,656 - +0.5 635,835 + GFF589 0.20 +1.4 635,835 + GFF589 0.20 -0.0 635,835 + GFF589 0.20 -0.3 635,835 + GFF589 0.20 -1.2 635,835 + GFF589 0.20 +0.0 635,835 + GFF589 0.20 -1.2 635,835 + GFF589 0.20 -1.2 635,835 + GFF589 0.20 -0.9 635,835 + GFF589 0.20 -0.9 635,835 + GFF589 0.20 -0.3 635,836 - GFF589 0.20 -0.4 635,836 - GFF589 0.20 -0.6 635,836 - GFF589 0.20 +0.4 635,836 - GFF589 0.20 +0.8 635,836 - GFF589 0.20 -0.8 635,898 + GFF589 0.29 +0.1 635,899 - GFF589 0.29 -0.3 635,899 - GFF589 0.29 +1.1 635,899 - GFF589 0.29 -0.6 636,007 - GFF589 0.45 -0.3
Or see this region's nucleotide sequence