Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5861 and GFF5862 overlap by 11 nucleotides GFF5862 and GFF5863 are separated by 300 nucleotides
GFF5861 - Fe2+-dicitrate sensor, membrane component, at 63,493 to 64,506
GFF5861
GFF5862 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 64,496 to 65,005
GFF5862
GFF5863 - Ferrichrome-iron receptor, at 65,306 to 67,663
GFF5863
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 63.518 kb on + strand at 63.518 kb on + strand at 64.506 kb on + strand at 64.506 kb on + strand at 64.506 kb on + strand at 64.506 kb on + strand at 64.506 kb on + strand at 64.506 kb on + strand at 64.507 kb on - strand at 64.507 kb on - strand at 64.507 kb on - strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.518 kb on + strand at 64.519 kb on - strand at 64.519 kb on - strand at 64.519 kb on - strand at 64.519 kb on - strand at 64.530 kb on + strand at 64.530 kb on + strand at 64.530 kb on + strand at 64.596 kb on + strand, within GFF5862 at 64.596 kb on + strand, within GFF5862 at 64.596 kb on + strand, within GFF5862 at 64.596 kb on + strand, within GFF5862 at 64.596 kb on + strand, within GFF5862 at 64.596 kb on + strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.597 kb on - strand, within GFF5862 at 64.755 kb on + strand, within GFF5862 at 64.755 kb on + strand, within GFF5862 at 64.755 kb on + strand, within GFF5862 at 64.755 kb on + strand, within GFF5862 at 64.755 kb on + strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.756 kb on - strand, within GFF5862 at 64.785 kb on + strand, within GFF5862 at 64.785 kb on + strand, within GFF5862 at 64.785 kb on + strand, within GFF5862 at 64.785 kb on + strand, within GFF5862 at 64.786 kb on - strand, within GFF5862 at 64.786 kb on - strand, within GFF5862 at 64.786 kb on - strand, within GFF5862 at 64.786 kb on - strand, within GFF5862 at 64.786 kb on - strand, within GFF5862 at 64.821 kb on + strand, within GFF5862 at 64.821 kb on + strand, within GFF5862 at 64.821 kb on + strand, within GFF5862 at 64.822 kb on - strand, within GFF5862 at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.962 kb on + strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 64.963 kb on - strand at 65.066 kb on + strand at 65.067 kb on - strand at 65.068 kb on + strand at 65.068 kb on + strand at 65.068 kb on + strand at 65.068 kb on + strand at 65.068 kb on + strand at 65.068 kb on + strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.069 kb on - strand at 65.136 kb on + strand at 65.137 kb on - strand at 65.154 kb on + strand at 65.154 kb on + strand at 65.155 kb on - strand at 65.191 kb on - strand at 65.237 kb on + strand at 65.238 kb on - strand at 65.238 kb on - strand at 65.238 kb on - strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.305 kb on + strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.306 kb on - strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.312 kb on + strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.313 kb on - strand at 65.345 kb on + strand at 65.453 kb on + strand at 65.453 kb on + strand at 65.453 kb on + strand at 65.453 kb on + strand at 65.454 kb on - strand at 65.454 kb on - strand at 65.454 kb on - strand at 65.454 kb on - strand at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.675 kb on + strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.676 kb on - strand, within GFF5863 at 65.867 kb on + strand, within GFF5863 at 65.867 kb on + strand, within GFF5863 at 65.868 kb on - strand, within GFF5863 at 65.868 kb on - strand, within GFF5863 at 65.868 kb on - strand, within GFF5863
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 63,518 + +0.2 63,518 + +0.7 64,506 + -0.1 64,506 + -0.5 64,506 + -0.1 64,506 + +0.0 64,506 + +0.7 64,506 + +0.0 64,507 - -1.0 64,507 - +0.4 64,507 - +0.6 64,518 + -0.5 64,518 + +0.4 64,518 + +0.1 64,518 + +0.1 64,518 + -1.2 64,518 + -0.7 64,518 + -0.3 64,519 - -0.7 64,519 - -0.7 64,519 - +0.1 64,519 - -0.5 64,530 + -0.7 64,530 + -0.4 64,530 + +0.3 64,596 + GFF5862 0.20 +0.7 64,596 + GFF5862 0.20 +0.0 64,596 + GFF5862 0.20 +0.2 64,596 + GFF5862 0.20 +1.0 64,596 + GFF5862 0.20 +1.4 64,596 + GFF5862 0.20 +0.0 64,597 - GFF5862 0.20 +0.9 64,597 - GFF5862 0.20 -0.6 64,597 - GFF5862 0.20 +0.1 64,597 - GFF5862 0.20 -0.6 64,597 - GFF5862 0.20 +0.0 64,597 - GFF5862 0.20 +0.3 64,597 - GFF5862 0.20 -0.1 64,597 - GFF5862 0.20 -0.2 64,597 - GFF5862 0.20 -0.8 64,597 - GFF5862 0.20 -1.9 64,755 + GFF5862 0.51 +0.2 64,755 + GFF5862 0.51 +1.2 64,755 + GFF5862 0.51 -0.4 64,755 + GFF5862 0.51 -1.3 64,755 + GFF5862 0.51 -0.2 64,756 - GFF5862 0.51 +1.2 64,756 - GFF5862 0.51 +0.6 64,756 - GFF5862 0.51 +0.7 64,756 - GFF5862 0.51 -0.5 64,756 - GFF5862 0.51 -0.9 64,756 - GFF5862 0.51 -1.0 64,756 - GFF5862 0.51 +1.0 64,756 - GFF5862 0.51 +0.7 64,756 - GFF5862 0.51 +0.5 64,756 - GFF5862 0.51 +1.3 64,785 + GFF5862 0.57 +1.6 64,785 + GFF5862 0.57 +0.3 64,785 + GFF5862 0.57 -0.2 64,785 + GFF5862 0.57 -1.2 64,786 - GFF5862 0.57 +0.9 64,786 - GFF5862 0.57 -0.5 64,786 - GFF5862 0.57 -0.4 64,786 - GFF5862 0.57 +0.4 64,786 - GFF5862 0.57 -3.0 64,821 + GFF5862 0.64 -0.2 64,821 + GFF5862 0.64 -0.1 64,821 + GFF5862 0.64 +0.3 64,822 - GFF5862 0.64 -1.3 64,962 + -0.3 64,962 + -1.5 64,962 + -0.1 64,962 + -0.6 64,962 + -0.3 64,962 + +0.3 64,962 + +0.6 64,962 + +0.3 64,962 + -0.7 64,963 - +0.2 64,963 - -1.4 64,963 - -0.7 64,963 - -0.7 64,963 - -0.7 64,963 - -1.5 64,963 - -0.8 64,963 - +0.0 64,963 - +0.2 64,963 - +0.1 65,066 + -1.4 65,067 - -0.6 65,068 + +0.3 65,068 + -1.1 65,068 + +0.5 65,068 + -0.3 65,068 + -1.3 65,068 + +0.5 65,069 - +1.3 65,069 - -0.4 65,069 - +0.2 65,069 - +0.2 65,069 - -0.9 65,069 - -0.2 65,069 - -0.3 65,069 - -0.6 65,069 - -0.8 65,136 + -0.9 65,137 - +0.3 65,154 + +0.1 65,154 + +0.9 65,155 - +0.2 65,191 - -0.7 65,237 + +0.9 65,238 - -1.5 65,238 - -0.3 65,238 - +0.4 65,305 + +0.1 65,305 + -3.4 65,305 + -1.5 65,305 + -1.1 65,305 + +0.3 65,305 + -0.6 65,305 + -1.2 65,305 + -0.1 65,305 + -0.9 65,305 + +0.7 65,306 - +0.1 65,306 - -0.5 65,306 - +0.3 65,306 - -0.6 65,306 - +0.5 65,306 - +0.4 65,306 - -0.9 65,306 - +1.9 65,306 - +0.4 65,306 - -1.4 65,312 + -0.4 65,312 + +1.1 65,312 + -0.7 65,312 + +0.1 65,312 + -0.2 65,312 + +0.5 65,312 + +1.3 65,312 + -0.0 65,312 + -0.7 65,312 + +0.3 65,312 + -0.1 65,312 + +1.1 65,312 + -0.0 65,312 + -0.7 65,312 + +0.6 65,312 + +0.1 65,312 + +0.5 65,312 + -0.6 65,312 + -1.3 65,312 + -0.9 65,312 + -0.5 65,312 + +0.7 65,312 + -0.4 65,312 + -0.2 65,312 + -0.1 65,312 + -0.7 65,313 - +0.8 65,313 - -0.6 65,313 - -0.5 65,313 - -0.2 65,313 - -0.6 65,313 - +1.2 65,313 - -1.3 65,313 - +1.6 65,313 - +0.1 65,313 - -1.4 65,313 - -0.5 65,313 - -1.6 65,313 - +0.7 65,313 - +0.6 65,345 + -0.0 65,453 + -1.5 65,453 + +0.3 65,453 + +0.3 65,453 + -1.3 65,454 - +0.1 65,454 - +0.1 65,454 - +0.2 65,454 - -0.1 65,675 + GFF5863 0.16 -0.2 65,675 + GFF5863 0.16 -0.3 65,675 + GFF5863 0.16 +0.3 65,675 + GFF5863 0.16 -0.8 65,675 + GFF5863 0.16 +0.4 65,675 + GFF5863 0.16 -0.9 65,675 + GFF5863 0.16 +0.6 65,675 + GFF5863 0.16 -0.5 65,675 + GFF5863 0.16 -0.7 65,675 + GFF5863 0.16 -0.5 65,675 + GFF5863 0.16 +0.9 65,675 + GFF5863 0.16 -0.1 65,675 + GFF5863 0.16 +0.3 65,675 + GFF5863 0.16 -0.5 65,675 + GFF5863 0.16 +0.7 65,675 + GFF5863 0.16 -1.0 65,676 - GFF5863 0.16 -0.6 65,676 - GFF5863 0.16 +0.1 65,676 - GFF5863 0.16 +0.2 65,676 - GFF5863 0.16 -0.7 65,676 - GFF5863 0.16 -0.3 65,676 - GFF5863 0.16 -0.6 65,676 - GFF5863 0.16 -0.4 65,676 - GFF5863 0.16 -1.2 65,676 - GFF5863 0.16 +0.9 65,676 - GFF5863 0.16 -0.2 65,676 - GFF5863 0.16 -0.3 65,676 - GFF5863 0.16 +1.0 65,676 - GFF5863 0.16 -0.8 65,676 - GFF5863 0.16 -2.0 65,676 - GFF5863 0.16 -0.3 65,676 - GFF5863 0.16 -0.5 65,676 - GFF5863 0.16 +0.8 65,676 - GFF5863 0.16 -0.9 65,676 - GFF5863 0.16 +0.0 65,867 + GFF5863 0.24 -0.9 65,867 + GFF5863 0.24 -0.6 65,868 - GFF5863 0.24 -0.5 65,868 - GFF5863 0.24 +0.1 65,868 - GFF5863 0.24 +0.3
Or see this region's nucleotide sequence