Strain Fitness in Variovorax sp. SCN45 around GFF5852

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
30,035	+		GFF5851	0.31	+0.3
30,692	+		GFF5851	0.82	-0.9
30,692	+		GFF5851	0.82	+0.6
30,692	+		GFF5851	0.82	-1.4
30,693	-		GFF5851	0.82	-0.1
30,701	+		GFF5851	0.83	-0.4
30,701	+		GFF5851	0.83	-2.0
30,702	-		GFF5851	0.83	-0.0
30,915	+				-0.6
30,915	+				-0.2
30,915	+				-0.0
30,916	-				+0.9
30,916	-				+0.9
30,972	+				+0.1
30,972	+				+0.0
30,973	-				+2.4
30,973	-				-0.2
30,973	-				-0.1
31,087	-		GFF5852	0.13	-0.1
31,087	-		GFF5852	0.13	-1.5
31,087	-		GFF5852	0.13	+0.1
31,087	-		GFF5852	0.13	-0.5
31,087	-		GFF5852	0.13	+0.5
31,087	-		GFF5852	0.13	-1.5
31,087	-		GFF5852	0.13	-0.6
31,096	-		GFF5852	0.14	+0.4
31,099	-		GFF5852	0.14	+0.4
31,099	-		GFF5852	0.14	-0.9
31,099	-		GFF5852	0.14	+1.1
31,099	-		GFF5852	0.14	-0.6
31,102	-		GFF5852	0.14	-0.7
31,102	-		GFF5852	0.14	-1.1
31,102	-		GFF5852	0.14	+0.7
31,102	-		GFF5852	0.14	-0.2
31,174	-		GFF5852	0.20	-0.3
31,182	+		GFF5852	0.21	-0.5
31,183	-		GFF5852	0.21	-0.8
31,189	-		GFF5852	0.21	-0.7
31,189	-		GFF5852	0.21	-0.8
31,189	-		GFF5852	0.21	-0.5
31,476	+		GFF5852	0.44	+0.2
31,476	+		GFF5852	0.44	+0.1
31,479	+		GFF5852	0.44	-0.5
31,735	+		GFF5852	0.65	-0.1
31,736	-		GFF5852	0.65	+0.4
32,866	+		GFF5853	0.33	-1.3