Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5522 and GFF5523 are separated by 55 nucleotides GFF5523 and GFF5524 are separated by 6 nucleotides GFF5524 and GFF5525 are separated by 142 nucleotides
GFF5522 - Uracil-DNA glycosylase, family 4 (EC 3.2.2.27), at 152,225 to 153,067
GFF5522
GFF5523 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 153,123 to 153,947
GFF5523
GFF5524 - Predicted transcriptional regulator, at 153,954 to 154,688
GFF5524
GFF5525 - FIG00933173: hypothetical protein, at 154,831 to 155,685
GFF5525
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 153.109 kb on + strand at 153.520 kb on - strand, within GFF5523 at 153.631 kb on - strand, within GFF5523 at 153.682 kb on + strand, within GFF5523 at 153.682 kb on + strand, within GFF5523 at 153.683 kb on - strand, within GFF5523 at 153.706 kb on - strand, within GFF5523 at 153.861 kb on + strand, within GFF5523 at 153.862 kb on - strand, within GFF5523 at 153.862 kb on - strand, within GFF5523 at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.018 kb on + strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.019 kb on - strand at 154.183 kb on + strand, within GFF5524 at 154.183 kb on + strand, within GFF5524 at 154.183 kb on + strand, within GFF5524 at 154.184 kb on - strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.186 kb on + strand, within GFF5524 at 154.187 kb on - strand, within GFF5524 at 154.187 kb on - strand, within GFF5524 at 154.187 kb on - strand, within GFF5524 at 154.187 kb on - strand, within GFF5524 at 154.349 kb on + strand, within GFF5524 at 154.349 kb on + strand, within GFF5524 at 154.349 kb on + strand, within GFF5524 at 154.349 kb on + strand, within GFF5524 at 154.349 kb on + strand, within GFF5524 at 154.350 kb on - strand, within GFF5524 at 154.350 kb on - strand, within GFF5524 at 154.350 kb on - strand, within GFF5524 at 154.354 kb on + strand, within GFF5524 at 154.354 kb on + strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.355 kb on - strand, within GFF5524 at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.624 kb on + strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.625 kb on - strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.665 kb on + strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.666 kb on - strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on - strand at 154.700 kb on - strand at 154.700 kb on - strand at 154.700 kb on - strand at 154.747 kb on + strand at 154.748 kb on - strand at 154.748 kb on - strand at 154.750 kb on + strand at 154.792 kb on + strand at 155.041 kb on + strand, within GFF5525 at 155.042 kb on - strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.191 kb on + strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.192 kb on - strand, within GFF5525 at 155.452 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.488 kb on + strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.489 kb on - strand, within GFF5525 at 155.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 153,109 + -2.0 153,520 - GFF5523 0.48 -0.8 153,631 - GFF5523 0.62 +0.0 153,682 + GFF5523 0.68 +0.4 153,682 + GFF5523 0.68 +0.0 153,683 - GFF5523 0.68 +0.4 153,706 - GFF5523 0.71 +1.0 153,861 + GFF5523 0.89 -0.3 153,862 - GFF5523 0.90 -1.0 153,862 - GFF5523 0.90 -1.8 154,018 + -0.9 154,018 + +0.6 154,018 + +1.4 154,018 + +1.3 154,018 + +0.9 154,018 + +0.2 154,018 + +0.5 154,018 + +0.0 154,018 + +0.6 154,018 + -0.3 154,018 + -0.5 154,018 + +0.1 154,018 + -0.6 154,018 + -0.9 154,018 + +0.1 154,018 + +2.6 154,018 + +0.7 154,019 - +0.3 154,019 - +0.6 154,019 - +0.2 154,019 - +0.7 154,019 - +0.3 154,019 - -0.4 154,019 - +0.0 154,019 - -0.6 154,019 - +0.3 154,019 - +0.9 154,019 - +0.8 154,019 - -0.2 154,183 + GFF5524 0.31 -0.4 154,183 + GFF5524 0.31 -0.6 154,183 + GFF5524 0.31 +0.0 154,184 - GFF5524 0.31 -0.6 154,186 + GFF5524 0.32 +1.0 154,186 + GFF5524 0.32 +0.1 154,186 + GFF5524 0.32 -0.4 154,186 + GFF5524 0.32 -1.0 154,186 + GFF5524 0.32 -0.8 154,186 + GFF5524 0.32 -0.8 154,186 + GFF5524 0.32 -0.5 154,186 + GFF5524 0.32 -0.2 154,187 - GFF5524 0.32 +0.4 154,187 - GFF5524 0.32 -2.2 154,187 - GFF5524 0.32 -1.0 154,187 - GFF5524 0.32 +0.0 154,349 + GFF5524 0.54 -3.0 154,349 + GFF5524 0.54 +1.0 154,349 + GFF5524 0.54 -0.1 154,349 + GFF5524 0.54 -0.4 154,349 + GFF5524 0.54 +0.1 154,350 - GFF5524 0.54 +0.0 154,350 - GFF5524 0.54 +0.5 154,350 - GFF5524 0.54 +0.0 154,354 + GFF5524 0.54 +0.1 154,354 + GFF5524 0.54 -0.3 154,355 - GFF5524 0.55 -0.1 154,355 - GFF5524 0.55 -1.5 154,355 - GFF5524 0.55 +0.8 154,355 - GFF5524 0.55 -1.7 154,355 - GFF5524 0.55 -1.1 154,355 - GFF5524 0.55 -0.1 154,624 + -1.1 154,624 + -1.3 154,624 + -0.1 154,624 + -1.7 154,624 + -0.5 154,624 + +0.0 154,624 + -0.2 154,624 + -0.1 154,624 + +0.2 154,624 + -0.6 154,625 - +0.4 154,625 - +0.5 154,625 - +0.2 154,625 - +0.7 154,625 - -0.2 154,625 - -0.6 154,625 - -0.3 154,625 - +0.5 154,665 + +0.4 154,665 + +0.1 154,665 + -0.3 154,665 + -0.4 154,665 + -2.3 154,665 + -0.3 154,665 + +1.0 154,665 + -1.0 154,665 + +0.1 154,665 + -0.3 154,665 + +0.6 154,665 + +0.3 154,665 + +0.2 154,665 + -0.7 154,665 + +1.0 154,665 + +0.6 154,665 + -0.2 154,665 + -0.3 154,666 - -2.1 154,666 - +0.7 154,666 - -2.1 154,666 - -0.0 154,666 - -0.9 154,666 - -1.9 154,666 - -0.6 154,666 - +1.8 154,666 - +0.2 154,699 + -0.5 154,699 + -0.4 154,699 + -1.5 154,699 - -0.6 154,700 - -1.2 154,700 - +0.2 154,700 - +0.7 154,747 + +2.6 154,748 - +0.2 154,748 - -0.1 154,750 + -1.5 154,792 + +0.4 155,041 + GFF5525 0.25 -1.5 155,042 - GFF5525 0.25 -0.2 155,191 + GFF5525 0.42 -0.2 155,191 + GFF5525 0.42 -0.9 155,191 + GFF5525 0.42 -0.2 155,191 + GFF5525 0.42 -0.0 155,191 + GFF5525 0.42 -1.5 155,191 + GFF5525 0.42 +0.0 155,191 + GFF5525 0.42 +0.6 155,191 + GFF5525 0.42 -0.2 155,191 + GFF5525 0.42 -0.9 155,191 + GFF5525 0.42 -0.4 155,191 + GFF5525 0.42 +0.5 155,191 + GFF5525 0.42 -0.5 155,191 + GFF5525 0.42 -0.3 155,191 + GFF5525 0.42 -0.3 155,191 + GFF5525 0.42 -0.4 155,191 + GFF5525 0.42 -0.3 155,191 + GFF5525 0.42 -0.8 155,192 - GFF5525 0.42 +0.2 155,192 - GFF5525 0.42 -2.6 155,192 - GFF5525 0.42 +0.6 155,192 - GFF5525 0.42 -0.1 155,192 - GFF5525 0.42 +0.6 155,192 - GFF5525 0.42 +1.6 155,192 - GFF5525 0.42 -0.1 155,192 - GFF5525 0.42 +0.4 155,192 - GFF5525 0.42 -1.1 155,192 - GFF5525 0.42 -0.8 155,192 - GFF5525 0.42 -1.1 155,192 - GFF5525 0.42 -0.3 155,192 - GFF5525 0.42 +0.2 155,192 - GFF5525 0.42 +0.7 155,452 + GFF5525 0.73 -0.4 155,488 + GFF5525 0.77 -0.8 155,488 + GFF5525 0.77 -0.1 155,488 + GFF5525 0.77 -0.4 155,488 + GFF5525 0.77 -0.0 155,488 + GFF5525 0.77 -0.3 155,488 + GFF5525 0.77 -1.0 155,489 - GFF5525 0.77 +0.9 155,489 - GFF5525 0.77 -0.0 155,489 - GFF5525 0.77 -1.0 155,489 - GFF5525 0.77 -0.5 155,489 - GFF5525 0.77 +0.4 155,489 - GFF5525 0.77 -0.4 155,489 - GFF5525 0.77 +2.0 155,489 - GFF5525 0.77 -0.6 155,676 - -0.6
Or see this region's nucleotide sequence