Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5457 and GFF5458 are separated by 28 nucleotides GFF5458 and GFF5459 are separated by 5 nucleotides
GFF5457 - L-threonate dehydrogenase (NAD+), at 87,896 to 88,768
GFF5457
GFF5458 - D-erythronate dehydrogenase (NAD+), at 88,797 to 89,813
GFF5458
GFF5459 - 3-oxo-tetronate kinase, at 89,819 to 91,090
GFF5459
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-1
0
1 at 88.217 kb on + strand, within GFF5457 at 88.217 kb on + strand, within GFF5457 at 88.346 kb on + strand, within GFF5457 at 88.347 kb on - strand, within GFF5457 at 88.481 kb on + strand, within GFF5457 at 88.481 kb on + strand, within GFF5457 at 88.481 kb on + strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.482 kb on - strand, within GFF5457 at 88.556 kb on + strand, within GFF5457 at 88.556 kb on + strand, within GFF5457 at 88.557 kb on - strand, within GFF5457 at 88.557 kb on - strand, within GFF5457 at 88.557 kb on - strand, within GFF5457 at 88.557 kb on - strand, within GFF5457 at 88.691 kb on + strand at 88.692 kb on - strand at 89.263 kb on - strand, within GFF5458 at 89.263 kb on - strand, within GFF5458 at 89.263 kb on - strand, within GFF5458 at 89.764 kb on - strand at 89.764 kb on - strand at 89.764 kb on - strand at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.062 kb on + strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.063 kb on - strand, within GFF5459 at 90.071 kb on + strand, within GFF5459 at 90.071 kb on + strand, within GFF5459 at 90.072 kb on - strand, within GFF5459 at 90.163 kb on + strand, within GFF5459 at 90.164 kb on - strand, within GFF5459 at 90.164 kb on - strand, within GFF5459 at 90.206 kb on + strand, within GFF5459 at 90.206 kb on + strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.207 kb on - strand, within GFF5459 at 90.336 kb on - strand, within GFF5459 at 90.753 kb on - strand, within GFF5459
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 88,217 + GFF5457 0.37 +1.0 88,217 + GFF5457 0.37 -0.6 88,346 + GFF5457 0.52 -0.4 88,347 - GFF5457 0.52 +0.2 88,481 + GFF5457 0.67 -0.5 88,481 + GFF5457 0.67 +0.0 88,481 + GFF5457 0.67 -0.6 88,482 - GFF5457 0.67 -0.1 88,482 - GFF5457 0.67 -0.6 88,482 - GFF5457 0.67 -0.3 88,482 - GFF5457 0.67 -0.6 88,482 - GFF5457 0.67 -1.0 88,482 - GFF5457 0.67 -0.6 88,482 - GFF5457 0.67 -1.1 88,482 - GFF5457 0.67 -1.1 88,482 - GFF5457 0.67 -0.4 88,556 + GFF5457 0.76 -0.5 88,556 + GFF5457 0.76 -0.6 88,557 - GFF5457 0.76 +0.4 88,557 - GFF5457 0.76 -0.4 88,557 - GFF5457 0.76 -0.9 88,557 - GFF5457 0.76 -0.9 88,691 + -1.7 88,692 - -0.6 89,263 - GFF5458 0.46 -0.1 89,263 - GFF5458 0.46 +0.7 89,263 - GFF5458 0.46 +1.1 89,764 - +0.4 89,764 - -0.2 89,764 - -1.0 90,062 + GFF5459 0.19 -0.5 90,062 + GFF5459 0.19 -0.5 90,062 + GFF5459 0.19 +0.0 90,062 + GFF5459 0.19 -0.1 90,062 + GFF5459 0.19 -1.3 90,062 + GFF5459 0.19 -0.5 90,062 + GFF5459 0.19 +0.7 90,062 + GFF5459 0.19 -0.1 90,062 + GFF5459 0.19 +0.3 90,063 - GFF5459 0.19 +0.6 90,063 - GFF5459 0.19 -0.7 90,063 - GFF5459 0.19 -0.1 90,071 + GFF5459 0.20 +0.3 90,071 + GFF5459 0.20 +0.1 90,072 - GFF5459 0.20 -0.6 90,163 + GFF5459 0.27 +0.8 90,164 - GFF5459 0.27 +0.0 90,164 - GFF5459 0.27 +1.3 90,206 + GFF5459 0.30 -0.1 90,206 + GFF5459 0.30 -0.9 90,207 - GFF5459 0.31 +1.0 90,207 - GFF5459 0.31 +0.7 90,207 - GFF5459 0.31 -0.8 90,207 - GFF5459 0.31 +1.1 90,336 - GFF5459 0.41 -0.8 90,753 - GFF5459 0.73 +0.0
Or see this region's nucleotide sequence