Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4960 and GFF4961 overlap by 4 nucleotides GFF4961 and GFF4962 are separated by 7 nucleotides GFF4962 and GFF4963 are separated by 76 nucleotides
GFF4960 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,789 to 61,544
GFF4960
GFF4961 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 61,541 to 62,260
GFF4961
GFF4962 - Multidrug resistance transporter, Bcr/CflA family, at 62,268 to 63,476
GFF4962
GFF4963 - Transcriptional regulator, AraC family, at 63,553 to 64,503
GFF4963
Position (kb) 62
63
64 Strain fitness (log2 ratio) -1
0
1
2
3 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.133 kb on - strand, within GFF4961 at 62.133 kb on - strand, within GFF4961 at 62.258 kb on + strand at 62.259 kb on - strand at 62.259 kb on - strand at 62.259 kb on - strand at 62.524 kb on + strand, within GFF4962 at 62.599 kb on - strand, within GFF4962 at 62.599 kb on - strand, within GFF4962 at 62.599 kb on - strand, within GFF4962 at 62.726 kb on - strand, within GFF4962 at 62.726 kb on - strand, within GFF4962 at 62.782 kb on + strand, within GFF4962 at 62.783 kb on - strand, within GFF4962 at 62.860 kb on + strand, within GFF4962 at 63.047 kb on + strand, within GFF4962 at 63.047 kb on + strand, within GFF4962 at 63.048 kb on - strand, within GFF4962 at 63.049 kb on + strand, within GFF4962 at 63.049 kb on + strand, within GFF4962 at 63.049 kb on + strand, within GFF4962 at 63.049 kb on + strand, within GFF4962 at 63.050 kb on - strand, within GFF4962 at 63.050 kb on - strand, within GFF4962 at 63.050 kb on - strand, within GFF4962 at 63.050 kb on - strand, within GFF4962 at 63.097 kb on + strand, within GFF4962 at 63.097 kb on + strand, within GFF4962 at 63.097 kb on + strand, within GFF4962 at 63.098 kb on - strand, within GFF4962 at 63.098 kb on - strand, within GFF4962 at 63.098 kb on - strand, within GFF4962 at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.391 kb on + strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.392 kb on - strand at 63.652 kb on + strand, within GFF4963 at 63.863 kb on - strand, within GFF4963 at 63.863 kb on - strand, within GFF4963 at 64.312 kb on + strand, within GFF4963 at 64.313 kb on - strand, within GFF4963 at 64.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 62,132 + GFF4961 0.82 -1.1 62,132 + GFF4961 0.82 +2.5 62,132 + GFF4961 0.82 -0.0 62,132 + GFF4961 0.82 -0.3 62,133 - GFF4961 0.82 +0.0 62,133 - GFF4961 0.82 -0.1 62,258 + -0.5 62,259 - -0.4 62,259 - -0.6 62,259 - +0.5 62,524 + GFF4962 0.21 -0.1 62,599 - GFF4962 0.27 -0.4 62,599 - GFF4962 0.27 +0.8 62,599 - GFF4962 0.27 +1.5 62,726 - GFF4962 0.38 -0.3 62,726 - GFF4962 0.38 -0.4 62,782 + GFF4962 0.43 +0.0 62,783 - GFF4962 0.43 +0.6 62,860 + GFF4962 0.49 +1.2 63,047 + GFF4962 0.64 +0.4 63,047 + GFF4962 0.64 +0.3 63,048 - GFF4962 0.65 -0.4 63,049 + GFF4962 0.65 -0.1 63,049 + GFF4962 0.65 +1.7 63,049 + GFF4962 0.65 +0.3 63,049 + GFF4962 0.65 -0.5 63,050 - GFF4962 0.65 +0.6 63,050 - GFF4962 0.65 +0.9 63,050 - GFF4962 0.65 +0.6 63,050 - GFF4962 0.65 -0.1 63,097 + GFF4962 0.69 -0.1 63,097 + GFF4962 0.69 -0.3 63,097 + GFF4962 0.69 +0.3 63,098 - GFF4962 0.69 -0.6 63,098 - GFF4962 0.69 +1.2 63,098 - GFF4962 0.69 +0.3 63,391 + -0.5 63,391 + +0.9 63,391 + +0.4 63,391 + -1.1 63,391 + -0.3 63,391 + +3.1 63,392 - -0.4 63,392 - +1.1 63,392 - -0.9 63,392 - -0.5 63,392 - +0.8 63,392 - -0.5 63,392 - -1.1 63,392 - -0.3 63,392 - -0.2 63,652 + GFF4963 0.10 -0.5 63,863 - GFF4963 0.33 +0.2 63,863 - GFF4963 0.33 -0.4 64,312 + GFF4963 0.80 +0.5 64,313 - GFF4963 0.80 -0.3 64,467 + +0.5
Or see this region's nucleotide sequence