Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4619 and GFF4620 are separated by 26 nucleotides GFF4620 and GFF4621 are separated by 3 nucleotides GFF4621 and GFF4622 are separated by 17 nucleotides
GFF4619 - Altronate dehydratase (EC 4.2.1.7), at 260,520 to 262,058
GFF4619
GFF4620 - Gluconolactonase (EC 3.1.1.17), at 262,085 to 263,017
GFF4620
GFF4621 - Hydroxycarboxylate dehydrogenase (NADP+) HcxB, at 263,021 to 264,076
GFF4621
GFF4622 - Permease of the drug/metabolite transporter (DMT) superfamily, at 264,094 to 264,915
GFF4622
Position (kb) 263
264
265 Strain fitness (log2 ratio) -2
-1
0
1
2 at 262.056 kb on + strand at 262.056 kb on + strand at 262.056 kb on + strand at 262.056 kb on + strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.057 kb on - strand at 262.342 kb on + strand, within GFF4620 at 262.343 kb on - strand, within GFF4620 at 262.343 kb on - strand, within GFF4620 at 262.520 kb on + strand, within GFF4620 at 262.539 kb on - strand, within GFF4620 at 262.583 kb on + strand, within GFF4620 at 262.611 kb on + strand, within GFF4620 at 262.611 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.679 kb on + strand, within GFF4620 at 262.680 kb on - strand, within GFF4620 at 262.680 kb on - strand, within GFF4620 at 262.871 kb on + strand, within GFF4620 at 262.872 kb on - strand, within GFF4620 at 262.872 kb on - strand, within GFF4620 at 262.985 kb on + strand at 262.986 kb on - strand at 262.986 kb on - strand at 262.986 kb on - strand at 263.180 kb on + strand, within GFF4621 at 263.181 kb on - strand, within GFF4621 at 263.183 kb on - strand, within GFF4621 at 263.320 kb on - strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.555 kb on + strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.556 kb on - strand, within GFF4621 at 263.627 kb on + strand, within GFF4621 at 263.789 kb on - strand, within GFF4621 at 263.849 kb on - strand, within GFF4621 at 264.075 kb on - strand at 264.095 kb on + strand at 264.096 kb on - strand at 264.377 kb on - strand, within GFF4622 at 264.377 kb on - strand, within GFF4622 at 264.377 kb on - strand, within GFF4622 at 264.377 kb on - strand, within GFF4622 at 264.470 kb on + strand, within GFF4622 at 264.470 kb on + strand, within GFF4622 at 264.551 kb on + strand, within GFF4622 at 264.552 kb on - strand, within GFF4622 at 264.699 kb on - strand, within GFF4622 at 264.722 kb on + strand, within GFF4622 at 264.723 kb on - strand, within GFF4622 at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 265.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 262,056 + +0.8 262,056 + +0.2 262,056 + -0.4 262,056 + +0.0 262,057 - -0.9 262,057 - -1.6 262,057 - -1.4 262,057 - -0.9 262,057 - +1.8 262,057 - -0.1 262,057 - -0.5 262,057 - -0.4 262,057 - -0.1 262,057 - -0.1 262,342 + GFF4620 0.28 +0.4 262,343 - GFF4620 0.28 +0.5 262,343 - GFF4620 0.28 +0.0 262,520 + GFF4620 0.47 +1.1 262,539 - GFF4620 0.49 +1.2 262,583 + GFF4620 0.53 -0.3 262,611 + GFF4620 0.56 +0.8 262,611 + GFF4620 0.56 +0.0 262,679 + GFF4620 0.64 +0.2 262,679 + GFF4620 0.64 -1.3 262,679 + GFF4620 0.64 +0.1 262,679 + GFF4620 0.64 -0.6 262,679 + GFF4620 0.64 -1.3 262,680 - GFF4620 0.64 +0.0 262,680 - GFF4620 0.64 +0.3 262,871 + GFF4620 0.84 +1.1 262,872 - GFF4620 0.84 +0.2 262,872 - GFF4620 0.84 -0.2 262,985 + -0.6 262,986 - -0.9 262,986 - -0.3 262,986 - -0.7 263,180 + GFF4621 0.15 -0.3 263,181 - GFF4621 0.15 +0.1 263,183 - GFF4621 0.15 -0.2 263,320 - GFF4621 0.28 +1.2 263,555 + GFF4621 0.51 +0.0 263,555 + GFF4621 0.51 -0.1 263,555 + GFF4621 0.51 -0.1 263,556 - GFF4621 0.51 +0.8 263,556 - GFF4621 0.51 -1.1 263,556 - GFF4621 0.51 -0.6 263,627 + GFF4621 0.57 -2.3 263,789 - GFF4621 0.73 +1.0 263,849 - GFF4621 0.78 +1.5 264,075 - -0.3 264,095 + -1.1 264,096 - -0.9 264,377 - GFF4622 0.34 -1.5 264,377 - GFF4622 0.34 +0.2 264,377 - GFF4622 0.34 -0.4 264,377 - GFF4622 0.34 -0.2 264,470 + GFF4622 0.46 +0.9 264,470 + GFF4622 0.46 -0.8 264,551 + GFF4622 0.56 +0.8 264,552 - GFF4622 0.56 +0.2 264,699 - GFF4622 0.74 -0.1 264,722 + GFF4622 0.76 +0.2 264,723 - GFF4622 0.77 -0.8 264,924 + +0.8 264,924 + +0.6 264,924 + +0.1 264,924 + +0.4 264,924 + -0.5 264,924 + -0.0 264,925 - +0.0 264,925 - +1.4 264,925 - -0.3 264,925 - +0.8 264,925 - +2.2 264,925 - +0.1 264,925 - -0.5 264,925 - +0.7 264,925 - +0.8 265,067 + -0.2
Or see this region's nucleotide sequence