Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4356 and GFF4357 overlap by 4 nucleotides GFF4357 and GFF4358 are separated by 49 nucleotides GFF4358 and GFF4359 are separated by 180 nucleotides GFF4359 and GFF4360 are separated by 7 nucleotides GFF4360 and GFF4361 are separated by 99 nucleotides
GFF4356 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 279,286 to 280,458
GFF4356
GFF4357 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 280,455 to 281,240
GFF4357
GFF4358 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 281,290 to 282,078
GFF4358
GFF4359 - no description, at 282,259 to 282,576
GFF4359
GFF4360 - no description, at 282,584 to 282,853
GFF4360
GFF4361 - Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13), at 282,953 to 284,584
GFF4361
Position (kb) 281
282
283 Strain fitness (log2 ratio) -1
0
1 at 280.370 kb on + strand at 280.660 kb on - strand, within GFF4357 at 281.755 kb on + strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 281.756 kb on - strand, within GFF4358 at 282.123 kb on + strand at 282.135 kb on + strand at 282.557 kb on + strand at 282.558 kb on - strand at 282.558 kb on - strand at 282.558 kb on - strand at 282.660 kb on + strand, within GFF4360 at 282.661 kb on - strand, within GFF4360 at 283.034 kb on + strand at 283.034 kb on + strand at 283.066 kb on - strand at 283.066 kb on - strand at 283.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 280,370 + -0.8 280,660 - GFF4357 0.26 +1.1 281,755 + GFF4358 0.59 +0.2 281,756 - GFF4358 0.59 +1.7 281,756 - GFF4358 0.59 +0.4 281,756 - GFF4358 0.59 +1.2 281,756 - GFF4358 0.59 +0.0 282,123 + -0.5 282,135 + -0.0 282,557 + +0.1 282,558 - -0.8 282,558 - -1.2 282,558 - -0.6 282,660 + GFF4360 0.28 +0.2 282,661 - GFF4360 0.29 -1.0 283,034 + -0.2 283,034 + -0.8 283,066 - +0.6 283,066 - +0.2 283,071 + -0.8
Or see this region's nucleotide sequence