Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF409 and GFF410 are separated by 14 nucleotides GFF410 and GFF411 overlap by 4 nucleotides GFF411 and GFF412 are separated by 119 nucleotides
GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564
GFF409
GFF410 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 444,579 to 445,067
GFF410
GFF411 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 445,064 to 445,786
GFF411
GFF412 - Transcription-repair coupling factor, at 445,906 to 449,388
GFF412
Position (kb) 444
445
446 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.770 kb on + strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.944 kb on + strand, within GFF409 at 443.945 kb on - strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.113 kb on - strand, within GFF409 at 444.288 kb on - strand, within GFF409 at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 445.797 kb on - strand at 445.841 kb on + strand at 445.841 kb on + strand at 445.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 443,596 + GFF409 0.32 -1.8 443,596 + GFF409 0.32 +1.8 443,596 + GFF409 0.32 -1.8 443,596 + GFF409 0.32 -1.2 443,596 + GFF409 0.32 +0.4 443,597 - GFF409 0.32 -0.2 443,597 - GFF409 0.32 -3.0 443,597 - GFF409 0.32 -1.0 443,597 - GFF409 0.32 -0.2 443,597 - GFF409 0.32 -1.6 443,770 + GFF409 0.44 -2.7 443,771 - GFF409 0.44 -0.2 443,771 - GFF409 0.44 -3.1 443,771 - GFF409 0.44 -0.9 443,944 + GFF409 0.56 -1.4 443,945 - GFF409 0.56 -0.2 444,097 + GFF409 0.67 -0.6 444,097 + GFF409 0.67 +0.4 444,112 + GFF409 0.68 -1.4 444,112 + GFF409 0.68 -2.1 444,112 + GFF409 0.68 +0.4 444,113 - GFF409 0.68 -2.0 444,288 - GFF409 0.81 -0.6 444,491 + -0.2 444,491 + -0.6 444,491 + -2.4 444,491 + -0.6 444,492 - -0.2 444,492 - -1.6 444,492 - -0.2 444,492 - -3.1 444,492 - -0.2 444,513 + +1.8 444,513 + +1.4 444,513 + -2.2 444,514 - +2.4 444,514 - +1.4 444,514 - -0.4 444,514 - -0.6 444,514 - -0.3 444,514 - +1.0 444,514 - -0.3 445,797 - +1.4 445,841 + -0.3 445,841 + +0.2 445,896 + +0.1
Or see this region's nucleotide sequence