Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF407 and GFF408 are separated by 53 nucleotides GFF408 and GFF409 are separated by 74 nucleotides GFF409 and GFF410 are separated by 14 nucleotides GFF410 and GFF411 overlap by 4 nucleotides
GFF407 - no description, at 441,801 to 442,283
GFF407
GFF408 - Two-component system response regulator OmpR, at 442,337 to 443,068
GFF408
GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564
GFF409
GFF410 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 444,579 to 445,067
GFF410
GFF411 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 445,064 to 445,786
GFF411
Position (kb) 443
444
445 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 442.177 kb on + strand, within GFF407 at 442.178 kb on - strand, within GFF407 at 442.178 kb on - strand, within GFF407 at 442.248 kb on + strand at 442.253 kb on + strand at 442.253 kb on + strand at 442.254 kb on - strand at 442.254 kb on - strand at 442.266 kb on + strand at 442.266 kb on + strand at 442.266 kb on + strand at 442.267 kb on - strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.270 kb on + strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.271 kb on - strand at 442.316 kb on + strand at 442.332 kb on + strand at 442.333 kb on - strand at 442.416 kb on - strand, within GFF408 at 442.652 kb on + strand, within GFF408 at 442.653 kb on - strand, within GFF408 at 442.653 kb on - strand, within GFF408 at 443.012 kb on + strand at 443.012 kb on + strand at 443.012 kb on + strand at 443.013 kb on - strand at 443.013 kb on - strand at 443.013 kb on - strand at 443.039 kb on + strand at 443.039 kb on + strand at 443.040 kb on - strand at 443.040 kb on - strand at 443.293 kb on + strand, within GFF409 at 443.293 kb on + strand, within GFF409 at 443.365 kb on + strand, within GFF409 at 443.365 kb on + strand, within GFF409 at 443.365 kb on + strand, within GFF409 at 443.365 kb on + strand, within GFF409 at 443.366 kb on - strand, within GFF409 at 443.464 kb on + strand, within GFF409 at 443.465 kb on - strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.770 kb on + strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.944 kb on + strand, within GFF409 at 443.945 kb on - strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.113 kb on - strand, within GFF409 at 444.288 kb on - strand, within GFF409 at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 442,177 + GFF407 0.78 -0.3 442,178 - GFF407 0.78 -0.8 442,178 - GFF407 0.78 +0.0 442,248 + -0.4 442,253 + +0.2 442,253 + +0.4 442,254 - +0.0 442,254 - -1.6 442,266 + -0.1 442,266 + +0.1 442,266 + -0.1 442,267 - -0.6 442,270 + +0.2 442,270 + -0.0 442,270 + -0.1 442,270 + +0.9 442,270 + -0.4 442,270 + +0.4 442,270 + -0.0 442,270 + -0.3 442,270 + -0.5 442,270 + +1.2 442,271 - -0.3 442,271 - -2.3 442,271 - -0.2 442,271 - +0.6 442,271 - -2.8 442,271 - -0.8 442,271 - +0.4 442,271 - -0.5 442,271 - -0.2 442,316 + -1.5 442,332 + +0.5 442,333 - -1.4 442,416 - GFF408 0.11 -1.0 442,652 + GFF408 0.43 -1.6 442,653 - GFF408 0.43 -2.1 442,653 - GFF408 0.43 +0.0 443,012 + -2.0 443,012 + +0.0 443,012 + +1.4 443,013 - -1.8 443,013 - -0.6 443,013 - +1.4 443,039 + +0.4 443,039 + -1.0 443,040 - -1.0 443,040 - -2.0 443,293 + GFF409 0.11 +0.0 443,293 + GFF409 0.11 -1.2 443,365 + GFF409 0.16 -1.2 443,365 + GFF409 0.16 -0.4 443,365 + GFF409 0.16 -1.4 443,365 + GFF409 0.16 -2.6 443,366 - GFF409 0.16 -1.0 443,464 + GFF409 0.23 -1.4 443,465 - GFF409 0.23 -1.8 443,596 + GFF409 0.32 -1.8 443,596 + GFF409 0.32 +1.8 443,596 + GFF409 0.32 -1.8 443,596 + GFF409 0.32 -1.2 443,596 + GFF409 0.32 +0.4 443,597 - GFF409 0.32 -0.2 443,597 - GFF409 0.32 -3.0 443,597 - GFF409 0.32 -1.0 443,597 - GFF409 0.32 -0.2 443,597 - GFF409 0.32 -1.6 443,770 + GFF409 0.44 -2.7 443,771 - GFF409 0.44 -0.2 443,771 - GFF409 0.44 -3.1 443,771 - GFF409 0.44 -0.9 443,944 + GFF409 0.56 -1.4 443,945 - GFF409 0.56 -0.2 444,097 + GFF409 0.67 -0.6 444,097 + GFF409 0.67 +0.4 444,112 + GFF409 0.68 -1.4 444,112 + GFF409 0.68 -2.1 444,112 + GFF409 0.68 +0.4 444,113 - GFF409 0.68 -2.0 444,288 - GFF409 0.81 -0.6 444,491 + -0.2 444,491 + -0.6 444,491 + -2.4 444,491 + -0.6 444,492 - -0.2 444,492 - -1.6 444,492 - -0.2 444,492 - -3.1 444,492 - -0.2 444,513 + +1.8 444,513 + +1.4 444,513 + -2.2 444,514 - +2.4 444,514 - +1.4 444,514 - -0.4 444,514 - -0.6 444,514 - -0.3 444,514 - +1.0 444,514 - -0.3
Or see this region's nucleotide sequence