Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3842 and GFF3843 are separated by 6 nucleotides GFF3843 and GFF3844 are separated by 108 nucleotides
GFF3842 - Uncharacterized protein EC-HemY in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 40,304 to 41,587
GFF3842
GFF3843 - Uncharacterized MFS-type transporter, at 41,594 to 43,162
GFF3843
GFF3844 - Transcriptional regulator, LysR family, at 43,271 to 44,137
GFF3844
Position (kb)
41
42
43
44 Strain fitness (log2 ratio)
-1
0
1 at 41.388 kb on - strand, within GFF3842 at 41.388 kb on - strand, within GFF3842 at 41.388 kb on - strand, within GFF3842 at 41.388 kb on - strand, within GFF3842 at 41.484 kb on - strand at 41.484 kb on - strand at 41.947 kb on + strand, within GFF3843 at 41.947 kb on + strand, within GFF3843 at 41.947 kb on + strand, within GFF3843 at 41.947 kb on + strand, within GFF3843 at 41.948 kb on - strand, within GFF3843 at 41.948 kb on - strand, within GFF3843 at 41.948 kb on - strand, within GFF3843 at 42.219 kb on - strand, within GFF3843 at 42.219 kb on - strand, within GFF3843 at 42.219 kb on - strand, within GFF3843 at 42.219 kb on - strand, within GFF3843 at 42.237 kb on + strand, within GFF3843 at 42.238 kb on - strand, within GFF3843 at 42.294 kb on + strand, within GFF3843 at 42.294 kb on + strand, within GFF3843 at 42.294 kb on + strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.295 kb on - strand, within GFF3843 at 42.306 kb on + strand, within GFF3843 at 42.306 kb on + strand, within GFF3843 at 42.306 kb on + strand, within GFF3843 at 42.306 kb on + strand, within GFF3843 at 42.307 kb on - strand, within GFF3843 at 42.307 kb on - strand, within GFF3843 at 42.307 kb on - strand, within GFF3843 at 42.307 kb on - strand, within GFF3843 at 42.307 kb on - strand, within GFF3843 at 42.394 kb on - strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.519 kb on + strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.520 kb on - strand, within GFF3843 at 42.946 kb on - strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on + strand, within GFF3843 at 42.999 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.000 kb on - strand, within GFF3843 at 43.246 kb on + strand at 43.260 kb on + strand at 43.475 kb on + strand, within GFF3844 at 43.820 kb on + strand, within GFF3844 at 44.007 kb on - strand, within GFF3844 at 44.007 kb on - strand, within GFF3844 at 44.007 kb on - strand, within GFF3844 at 44.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 41,388 - GFF3842 0.84 -0.4 41,388 - GFF3842 0.84 -0.6 41,388 - GFF3842 0.84 +0.4 41,388 - GFF3842 0.84 -1.0 41,484 - +0.5 41,484 - -0.1 41,947 + GFF3843 0.22 +0.0 41,947 + GFF3843 0.22 -0.1 41,947 + GFF3843 0.22 +0.1 41,947 + GFF3843 0.22 -1.6 41,948 - GFF3843 0.23 +0.7 41,948 - GFF3843 0.23 +0.8 41,948 - GFF3843 0.23 +1.1 42,219 - GFF3843 0.40 -0.9 42,219 - GFF3843 0.40 -0.1 42,219 - GFF3843 0.40 +0.1 42,219 - GFF3843 0.40 -0.2 42,237 + GFF3843 0.41 +0.1 42,238 - GFF3843 0.41 -1.1 42,294 + GFF3843 0.45 +0.9 42,294 + GFF3843 0.45 -0.2 42,294 + GFF3843 0.45 -0.4 42,295 - GFF3843 0.45 +1.1 42,295 - GFF3843 0.45 -0.4 42,295 - GFF3843 0.45 -0.5 42,295 - GFF3843 0.45 +0.4 42,295 - GFF3843 0.45 +0.1 42,295 - GFF3843 0.45 +0.4 42,295 - GFF3843 0.45 -0.7 42,306 + GFF3843 0.45 +0.1 42,306 + GFF3843 0.45 -0.8 42,306 + GFF3843 0.45 -0.3 42,306 + GFF3843 0.45 -0.1 42,307 - GFF3843 0.45 -0.6 42,307 - GFF3843 0.45 -0.6 42,307 - GFF3843 0.45 -0.1 42,307 - GFF3843 0.45 +0.1 42,307 - GFF3843 0.45 +0.5 42,394 - GFF3843 0.51 +0.4 42,519 + GFF3843 0.59 -0.9 42,519 + GFF3843 0.59 +0.6 42,519 + GFF3843 0.59 -0.2 42,519 + GFF3843 0.59 +1.2 42,519 + GFF3843 0.59 -1.1 42,519 + GFF3843 0.59 +0.3 42,520 - GFF3843 0.59 +0.0 42,520 - GFF3843 0.59 -0.7 42,520 - GFF3843 0.59 +0.5 42,520 - GFF3843 0.59 -0.0 42,520 - GFF3843 0.59 -0.6 42,520 - GFF3843 0.59 +1.1 42,520 - GFF3843 0.59 -0.3 42,520 - GFF3843 0.59 +0.6 42,520 - GFF3843 0.59 -0.1 42,946 - GFF3843 0.86 -0.1 42,999 + GFF3843 0.90 -1.4 42,999 + GFF3843 0.90 +0.1 42,999 + GFF3843 0.90 +1.1 42,999 + GFF3843 0.90 +0.1 42,999 + GFF3843 0.90 +0.7 42,999 + GFF3843 0.90 +0.2 42,999 + GFF3843 0.90 -0.1 42,999 + GFF3843 0.90 +0.1 42,999 + GFF3843 0.90 -0.5 42,999 - GFF3843 0.90 +0.7 43,000 - GFF3843 0.90 -0.4 43,000 - GFF3843 0.90 +0.7 43,000 - GFF3843 0.90 -0.6 43,000 - GFF3843 0.90 -0.2 43,000 - GFF3843 0.90 -0.9 43,000 - GFF3843 0.90 -0.7 43,000 - GFF3843 0.90 -1.1 43,000 - GFF3843 0.90 -0.5 43,246 + -0.5 43,260 + -0.2 43,475 + GFF3844 0.24 -0.8 43,820 + GFF3844 0.63 -0.4 44,007 - GFF3844 0.85 -0.2 44,007 - GFF3844 0.85 -0.4 44,007 - GFF3844 0.85 +0.7 44,124 - -0.6
Or see this region's nucleotide sequence