Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3814 and GFF3815 are separated by 15 nucleotides GFF3815 and GFF3816 are separated by 86 nucleotides GFF3816 and GFF3817 are separated by 28 nucleotides GFF3817 and GFF3818 are separated by 163 nucleotides
GFF3814 - OpgC protein, at 233 to 1,375
GFF3814
GFF3815 - FIG00347597: hypothetical protein, at 1,391 to 2,164
GFF3815
GFF3816 - hypothetical protein, at 2,251 to 3,021
GFF3816
GFF3817 - RNA polymerase sigma factor, at 3,050 to 3,643
GFF3817
GFF3818 - hypothetical protein, at 3,807 to 4,346
GFF3818
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.295 kb on + strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.296 kb on - strand at 1.470 kb on + strand, within GFF3815 at 1.470 kb on + strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.471 kb on - strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.508 kb on + strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.509 kb on - strand, within GFF3815 at 1.551 kb on + strand, within GFF3815 at 1.551 kb on + strand, within GFF3815 at 1.551 kb on + strand, within GFF3815 at 1.551 kb on + strand, within GFF3815 at 1.552 kb on - strand, within GFF3815 at 1.552 kb on - strand, within GFF3815 at 1.563 kb on + strand, within GFF3815 at 1.591 kb on - strand, within GFF3815 at 1.779 kb on + strand, within GFF3815 at 1.779 kb on + strand, within GFF3815 at 1.779 kb on + strand, within GFF3815 at 1.779 kb on + strand, within GFF3815 at 1.780 kb on - strand, within GFF3815 at 1.780 kb on - strand, within GFF3815 at 1.803 kb on + strand, within GFF3815 at 1.803 kb on + strand, within GFF3815 at 1.803 kb on + strand, within GFF3815 at 1.803 kb on + strand, within GFF3815 at 2.034 kb on + strand, within GFF3815 at 2.034 kb on + strand, within GFF3815 at 2.035 kb on - strand, within GFF3815 at 2.035 kb on - strand, within GFF3815 at 2.035 kb on - strand, within GFF3815 at 2.206 kb on - strand at 2.253 kb on - strand at 2.253 kb on - strand at 2.253 kb on - strand at 2.253 kb on - strand at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.582 kb on + strand, within GFF3816 at 2.583 kb on - strand, within GFF3816 at 2.583 kb on - strand, within GFF3816 at 2.583 kb on - strand, within GFF3816 at 2.583 kb on - strand, within GFF3816 at 2.583 kb on - strand, within GFF3816 at 2.903 kb on - strand, within GFF3816 at 3.021 kb on + strand at 3.171 kb on + strand, within GFF3817 at 3.172 kb on - strand, within GFF3817 at 3.172 kb on - strand, within GFF3817 at 3.172 kb on - strand, within GFF3817 at 3.172 kb on - strand, within GFF3817 at 3.172 kb on - strand, within GFF3817 at 3.423 kb on + strand, within GFF3817 at 3.540 kb on + strand, within GFF3817 at 3.541 kb on - strand, within GFF3817 at 3.541 kb on - strand, within GFF3817 at 3.661 kb on - strand at 3.661 kb on - strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.662 kb on + strand at 3.663 kb on - strand at 3.663 kb on - strand at 3.663 kb on - strand at 3.663 kb on - strand at 3.663 kb on - strand at 3.758 kb on + strand at 3.759 kb on + strand at 3.759 kb on + strand at 3.759 kb on + strand at 3.759 kb on + strand at 3.760 kb on - strand at 3.760 kb on - strand at 3.760 kb on - strand at 3.760 kb on - strand at 3.761 kb on + strand at 3.761 kb on + strand at 3.762 kb on - strand at 3.762 kb on - strand at 3.852 kb on + strand at 3.852 kb on + strand at 3.853 kb on - strand at 3.853 kb on - strand at 3.900 kb on + strand, within GFF3818 at 3.901 kb on - strand, within GFF3818 at 3.901 kb on - strand, within GFF3818 at 3.903 kb on + strand, within GFF3818 at 3.903 kb on + strand, within GFF3818 at 3.903 kb on + strand, within GFF3818 at 3.904 kb on - strand, within GFF3818 at 3.904 kb on - strand, within GFF3818 at 3.904 kb on - strand, within GFF3818 at 3.904 kb on - strand, within GFF3818 at 3.908 kb on + strand, within GFF3818 at 3.908 kb on + strand, within GFF3818 at 3.909 kb on - strand, within GFF3818
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 1,295 + +0.8 1,295 + -0.1 1,295 + +1.5 1,295 + -0.5 1,295 + -0.7 1,295 + +0.1 1,295 + +0.5 1,295 + -0.2 1,295 + -0.9 1,295 + +0.7 1,296 - -0.7 1,296 - +0.3 1,296 - +0.9 1,296 - +0.7 1,296 - -1.7 1,296 - -0.3 1,296 - -0.2 1,296 - +2.0 1,296 - +0.9 1,470 + GFF3815 0.10 -0.7 1,470 + GFF3815 0.10 +0.0 1,471 - GFF3815 0.10 -0.3 1,471 - GFF3815 0.10 +0.7 1,471 - GFF3815 0.10 +0.0 1,471 - GFF3815 0.10 +0.7 1,471 - GFF3815 0.10 +1.7 1,471 - GFF3815 0.10 -0.7 1,508 + GFF3815 0.15 -0.1 1,508 + GFF3815 0.15 +0.4 1,508 + GFF3815 0.15 +1.1 1,508 + GFF3815 0.15 -0.7 1,508 + GFF3815 0.15 +0.4 1,508 + GFF3815 0.15 -0.9 1,509 - GFF3815 0.15 +1.0 1,509 - GFF3815 0.15 -0.5 1,509 - GFF3815 0.15 -0.5 1,509 - GFF3815 0.15 -1.1 1,509 - GFF3815 0.15 +0.2 1,509 - GFF3815 0.15 -0.1 1,551 + GFF3815 0.21 -1.6 1,551 + GFF3815 0.21 +0.3 1,551 + GFF3815 0.21 +1.0 1,551 + GFF3815 0.21 -0.2 1,552 - GFF3815 0.21 -0.9 1,552 - GFF3815 0.21 +0.7 1,563 + GFF3815 0.22 -0.4 1,591 - GFF3815 0.26 -1.6 1,779 + GFF3815 0.50 -0.4 1,779 + GFF3815 0.50 -1.3 1,779 + GFF3815 0.50 -0.1 1,779 + GFF3815 0.50 -0.9 1,780 - GFF3815 0.50 +1.0 1,780 - GFF3815 0.50 +0.2 1,803 + GFF3815 0.53 -0.3 1,803 + GFF3815 0.53 -0.3 1,803 + GFF3815 0.53 -0.3 1,803 + GFF3815 0.53 -0.2 2,034 + GFF3815 0.83 -0.4 2,034 + GFF3815 0.83 +0.2 2,035 - GFF3815 0.83 +0.1 2,035 - GFF3815 0.83 +0.3 2,035 - GFF3815 0.83 -0.6 2,206 - -0.4 2,253 - +0.5 2,253 - -0.7 2,253 - +0.1 2,253 - -0.3 2,582 + GFF3816 0.43 +0.3 2,582 + GFF3816 0.43 -0.4 2,582 + GFF3816 0.43 -1.3 2,582 + GFF3816 0.43 -0.1 2,582 + GFF3816 0.43 +1.3 2,582 + GFF3816 0.43 -0.3 2,582 + GFF3816 0.43 +0.6 2,582 + GFF3816 0.43 +0.1 2,582 + GFF3816 0.43 -0.9 2,582 + GFF3816 0.43 -1.0 2,582 + GFF3816 0.43 -0.6 2,582 + GFF3816 0.43 +1.7 2,582 + GFF3816 0.43 +0.6 2,583 - GFF3816 0.43 -0.4 2,583 - GFF3816 0.43 -0.7 2,583 - GFF3816 0.43 -0.8 2,583 - GFF3816 0.43 -1.3 2,583 - GFF3816 0.43 +0.0 2,903 - GFF3816 0.85 -0.6 3,021 + +1.4 3,171 + GFF3817 0.20 +1.1 3,172 - GFF3817 0.21 -1.6 3,172 - GFF3817 0.21 +0.7 3,172 - GFF3817 0.21 +0.1 3,172 - GFF3817 0.21 -1.7 3,172 - GFF3817 0.21 +0.0 3,423 + GFF3817 0.63 -1.3 3,540 + GFF3817 0.82 +1.0 3,541 - GFF3817 0.83 +1.2 3,541 - GFF3817 0.83 +0.2 3,661 - +0.3 3,661 - +0.1 3,662 + -0.4 3,662 + +0.4 3,662 + -1.9 3,662 + -0.1 3,662 + -1.0 3,662 + +0.1 3,662 + -0.3 3,662 + +1.3 3,662 + +3.1 3,663 - +0.1 3,663 - -0.3 3,663 - +1.2 3,663 - +1.2 3,663 - -0.4 3,758 + -0.5 3,759 + +0.7 3,759 + -1.1 3,759 + -0.3 3,759 + -0.1 3,760 - -0.4 3,760 - -0.3 3,760 - -1.3 3,760 - -0.2 3,761 + +1.0 3,761 + +0.3 3,762 - -0.4 3,762 - -0.1 3,852 + -0.8 3,852 + +1.3 3,853 - +0.3 3,853 - +0.6 3,900 + GFF3818 0.17 +1.4 3,901 - GFF3818 0.17 +1.0 3,901 - GFF3818 0.17 +0.4 3,903 + GFF3818 0.18 -0.5 3,903 + GFF3818 0.18 -0.2 3,903 + GFF3818 0.18 -0.6 3,904 - GFF3818 0.18 -0.5 3,904 - GFF3818 0.18 +0.6 3,904 - GFF3818 0.18 -2.0 3,904 - GFF3818 0.18 -1.1 3,908 + GFF3818 0.19 +0.4 3,908 + GFF3818 0.19 -0.7 3,909 - GFF3818 0.19 +0.1
Or see this region's nucleotide sequence