Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3772 and GFF3773 are separated by 10 nucleotides GFF3773 and GFF3774 overlap by 4 nucleotides GFF3774 and GFF3775 overlap by 4 nucleotides GFF3775 and GFF3776 are separated by 167 nucleotides
GFF3772 - Arginase (EC 3.5.3.1), at 263,492 to 264,412
GFF3772
GFF3773 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 264,423 to 265,403
GFF3773
GFF3774 - hypothetical protein, at 265,400 to 266,797
GFF3774
GFF3775 - Two-component transcriptional response regulator, OmpR family, at 266,794 to 267,468
GFF3775
GFF3776 - no description, at 267,636 to 268,025
GFF3776
Position (kb) 265
266
267 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 264.658 kb on + strand, within GFF3773 at 264.658 kb on + strand, within GFF3773 at 264.658 kb on + strand, within GFF3773 at 264.659 kb on - strand, within GFF3773 at 264.836 kb on - strand, within GFF3773 at 264.985 kb on + strand, within GFF3773 at 264.985 kb on + strand, within GFF3773 at 264.985 kb on + strand, within GFF3773 at 264.985 kb on + strand, within GFF3773 at 264.986 kb on - strand, within GFF3773 at 264.986 kb on - strand, within GFF3773 at 264.986 kb on - strand, within GFF3773 at 264.993 kb on + strand, within GFF3773 at 264.993 kb on + strand, within GFF3773 at 264.993 kb on + strand, within GFF3773 at 264.994 kb on - strand, within GFF3773 at 264.994 kb on - strand, within GFF3773 at 264.994 kb on - strand, within GFF3773 at 264.994 kb on - strand, within GFF3773 at 264.994 kb on - strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.258 kb on + strand, within GFF3773 at 265.259 kb on - strand, within GFF3773 at 265.259 kb on - strand, within GFF3773 at 265.259 kb on - strand, within GFF3773 at 265.259 kb on - strand, within GFF3773 at 265.331 kb on - strand at 265.457 kb on - strand at 265.578 kb on + strand, within GFF3774 at 265.579 kb on - strand, within GFF3774 at 265.797 kb on + strand, within GFF3774 at 266.354 kb on + strand, within GFF3774 at 266.355 kb on - strand, within GFF3774 at 266.355 kb on - strand, within GFF3774 at 266.711 kb on + strand at 267.121 kb on + strand, within GFF3775 at 267.121 kb on + strand, within GFF3775 at 267.122 kb on - strand, within GFF3775 at 267.122 kb on - strand, within GFF3775 at 267.176 kb on + strand, within GFF3775 at 267.177 kb on - strand, within GFF3775 at 267.252 kb on + strand, within GFF3775 at 267.253 kb on - strand, within GFF3775 at 267.531 kb on + strand at 267.554 kb on - strand at 267.631 kb on + strand at 267.631 kb on + strand at 267.631 kb on + strand at 267.632 kb on - strand at 267.632 kb on - strand at 267.632 kb on - strand at 267.780 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.787 kb on - strand, within GFF3776 at 267.787 kb on - strand, within GFF3776 at 267.787 kb on - strand, within GFF3776
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 264,658 + GFF3773 0.24 -0.2 264,658 + GFF3773 0.24 -1.4 264,658 + GFF3773 0.24 -0.2 264,659 - GFF3773 0.24 -0.0 264,836 - GFF3773 0.42 +0.3 264,985 + GFF3773 0.57 -1.1 264,985 + GFF3773 0.57 -1.0 264,985 + GFF3773 0.57 +2.6 264,985 + GFF3773 0.57 -0.3 264,986 - GFF3773 0.57 +2.5 264,986 - GFF3773 0.57 -0.8 264,986 - GFF3773 0.57 -0.7 264,993 + GFF3773 0.58 +1.2 264,993 + GFF3773 0.58 -0.4 264,993 + GFF3773 0.58 -0.8 264,994 - GFF3773 0.58 -1.4 264,994 - GFF3773 0.58 +0.1 264,994 - GFF3773 0.58 -1.6 264,994 - GFF3773 0.58 +1.1 264,994 - GFF3773 0.58 -1.8 265,258 + GFF3773 0.85 -0.1 265,258 + GFF3773 0.85 +0.9 265,258 + GFF3773 0.85 -0.2 265,258 + GFF3773 0.85 -1.2 265,258 + GFF3773 0.85 -0.4 265,258 + GFF3773 0.85 -0.8 265,259 - GFF3773 0.85 -0.5 265,259 - GFF3773 0.85 +0.3 265,259 - GFF3773 0.85 -0.2 265,259 - GFF3773 0.85 -0.9 265,331 - -0.3 265,457 - -0.3 265,578 + GFF3774 0.13 +3.2 265,579 - GFF3774 0.13 +0.1 265,797 + GFF3774 0.28 -1.1 266,354 + GFF3774 0.68 -0.6 266,355 - GFF3774 0.68 +0.6 266,355 - GFF3774 0.68 -0.6 266,711 + -0.5 267,121 + GFF3775 0.48 -0.4 267,121 + GFF3775 0.48 -1.5 267,122 - GFF3775 0.49 +1.9 267,122 - GFF3775 0.49 -0.1 267,176 + GFF3775 0.57 -0.5 267,177 - GFF3775 0.57 +0.2 267,252 + GFF3775 0.68 +0.2 267,253 - GFF3775 0.68 +0.4 267,531 + +0.2 267,554 - +2.5 267,631 + +1.1 267,631 + +0.9 267,631 + +0.6 267,632 - -1.4 267,632 - +1.9 267,632 - -0.3 267,780 + GFF3776 0.37 -0.4 267,786 + GFF3776 0.38 -1.2 267,786 + GFF3776 0.38 -0.2 267,786 + GFF3776 0.38 +0.4 267,787 - GFF3776 0.39 +0.4 267,787 - GFF3776 0.39 -0.7 267,787 - GFF3776 0.39 +0.9
Or see this region's nucleotide sequence