Strain Fitness in Variovorax sp. SCN45 around GFF3525

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3524 and GFF3525 are separated by 86 nucleotidesGFF3525 and GFF3526 overlap by 4 nucleotidesGFF3526 and GFF3527 overlap by 4 nucleotides GFF3524 - Possible NADH oxidoreductase, at 30,358 to 31,494 GFF3524 GFF3525 - Protein tyrosine/serine phosphatase, at 31,581 to 32,117 GFF3525 GFF3526 - hypothetical protein, at 32,114 to 32,608 GFF3526 GFF3527 - UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase, at 32,605 to 33,750 GFF3527 Position (kb) 31 32 33Strain fitness (log2 ratio) -2 -1 0 1 2at 30.648 kb on - strand, within GFF3524at 30.648 kb on - strand, within GFF3524at 30.648 kb on - strand, within GFF3524at 30.648 kb on - strand, within GFF3524at 30.648 kb on - strand, within GFF3524at 30.653 kb on + strand, within GFF3524at 30.653 kb on + strand, within GFF3524at 30.654 kb on - strand, within GFF3524at 30.654 kb on - strand, within GFF3524at 30.654 kb on - strand, within GFF3524at 30.654 kb on - strand, within GFF3524at 30.654 kb on - strand, within GFF3524at 30.681 kb on - strand, within GFF3524at 30.689 kb on + strand, within GFF3524at 30.689 kb on + strand, within GFF3524at 30.689 kb on + strand, within GFF3524at 30.690 kb on - strand, within GFF3524at 30.690 kb on - strand, within GFF3524at 30.690 kb on - strand, within GFF3524at 30.690 kb on - strand, within GFF3524at 30.690 kb on - strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.848 kb on + strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.849 kb on - strand, within GFF3524at 30.900 kb on + strand, within GFF3524at 31.115 kb on + strand, within GFF3524at 31.125 kb on - strand, within GFF3524at 31.125 kb on - strand, within GFF3524at 31.549 kb on - strandat 31.549 kb on - strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.550 kb on + strandat 31.551 kb on - strandat 31.551 kb on - strandat 31.551 kb on - strandat 31.551 kb on - strandat 31.551 kb on - strandat 31.551 kb on - strandat 31.654 kb on + strand, within GFF3525at 31.684 kb on + strand, within GFF3525at 31.684 kb on + strand, within GFF3525at 31.753 kb on + strand, within GFF3525at 31.754 kb on - strand, within GFF3525at 31.754 kb on - strand, within GFF3525at 32.409 kb on + strand, within GFF3526at 32.410 kb on - strand, within GFF3526at 32.416 kb on - strand, within GFF3526at 32.563 kb on - strandat 32.733 kb on - strand, within GFF3527at 32.733 kb on - strand, within GFF3527at 32.733 kb on - strand, within GFF3527at 32.733 kb on - strand, within GFF3527at 32.990 kb on + strand, within GFF3527at 32.991 kb on - strand, within GFF3527at 32.991 kb on - strand, within GFF3527at 32.991 kb on - strand, within GFF3527

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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30,648 - GFF3524 0.26 -0.1
30,648 - GFF3524 0.26 -0.3
30,648 - GFF3524 0.26 -0.6
30,648 - GFF3524 0.26 -0.1
30,648 - GFF3524 0.26 +0.2
30,653 + GFF3524 0.26 -0.3
30,653 + GFF3524 0.26 -0.1
30,654 - GFF3524 0.26 -0.1
30,654 - GFF3524 0.26 +0.8
30,654 - GFF3524 0.26 -0.3
30,654 - GFF3524 0.26 +0.4
30,654 - GFF3524 0.26 +0.5
30,681 - GFF3524 0.28 -0.0
30,689 + GFF3524 0.29 -0.6
30,689 + GFF3524 0.29 -1.6
30,689 + GFF3524 0.29 -0.1
30,690 - GFF3524 0.29 -0.5
30,690 - GFF3524 0.29 +0.4
30,690 - GFF3524 0.29 +1.9
30,690 - GFF3524 0.29 +0.0
30,690 - GFF3524 0.29 -0.5
30,848 + GFF3524 0.43 -0.9
30,848 + GFF3524 0.43 -0.5
30,848 + GFF3524 0.43 -0.3
30,848 + GFF3524 0.43 +0.2
30,848 + GFF3524 0.43 +0.4
30,848 + GFF3524 0.43 -0.2
30,848 + GFF3524 0.43 -0.2
30,848 + GFF3524 0.43 -0.1
30,848 + GFF3524 0.43 +0.3
30,848 + GFF3524 0.43 +1.2
30,848 + GFF3524 0.43 -0.4
30,849 - GFF3524 0.43 -0.6
30,849 - GFF3524 0.43 -0.1
30,849 - GFF3524 0.43 -0.2
30,849 - GFF3524 0.43 +0.3
30,849 - GFF3524 0.43 +0.9
30,849 - GFF3524 0.43 -0.8
30,849 - GFF3524 0.43 -0.4
30,900 + GFF3524 0.48 -0.7
31,115 + GFF3524 0.67 -2.6
31,125 - GFF3524 0.67 -0.8
31,125 - GFF3524 0.67 +0.8
31,549 - +0.2
31,549 - -1.1
31,550 + -1.2
31,550 + -0.1
31,550 + +0.3
31,550 + -0.0
31,550 + -1.5
31,550 + +0.2
31,550 + -0.3
31,550 + -0.5
31,550 + +0.1
31,550 + -0.2
31,550 + -0.6
31,551 - +0.2
31,551 - -0.2
31,551 - -0.3
31,551 - +0.3
31,551 - -0.3
31,551 - +1.2
31,654 + GFF3525 0.14 -0.3
31,684 + GFF3525 0.19 +0.2
31,684 + GFF3525 0.19 +0.4
31,753 + GFF3525 0.32 +2.2
31,754 - GFF3525 0.32 +1.0
31,754 - GFF3525 0.32 -1.2
32,409 + GFF3526 0.60 -0.4
32,410 - GFF3526 0.60 -0.1
32,416 - GFF3526 0.61 -0.1
32,563 - -1.5
32,733 - GFF3527 0.11 -0.6
32,733 - GFF3527 0.11 +0.8
32,733 - GFF3527 0.11 -0.1
32,733 - GFF3527 0.11 -0.1
32,990 + GFF3527 0.34 +0.3
32,991 - GFF3527 0.34 -0.4
32,991 - GFF3527 0.34 -0.3
32,991 - GFF3527 0.34 +2.4

Or see this region's nucleotide sequence