Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3511 and GFF3512 are separated by 64 nucleotides GFF3512 and GFF3513 are separated by 130 nucleotides GFF3513 and GFF3514 overlap by 4 nucleotides GFF3514 and GFF3515 are separated by 1 nucleotides
GFF3511 - no description, at 17,174 to 18,160
GFF3511
GFF3512 - no description, at 18,225 to 18,578
GFF3512
GFF3513 - hypothetical protein, at 18,709 to 18,831
GFF3513
GFF3514 - no description, at 18,828 to 18,926
GFF3514
GFF3515 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 18,928 to 20,727
GFF3515
Position (kb) 18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 17.843 kb on + strand, within GFF3511 at 17.965 kb on + strand, within GFF3511 at 17.965 kb on + strand, within GFF3511 at 17.965 kb on + strand, within GFF3511 at 17.966 kb on - strand, within GFF3511 at 17.966 kb on - strand, within GFF3511 at 18.140 kb on + strand at 18.140 kb on + strand at 18.149 kb on + strand at 18.159 kb on - strand at 18.484 kb on - strand, within GFF3512 at 18.484 kb on - strand, within GFF3512 at 18.484 kb on - strand, within GFF3512 at 18.484 kb on - strand, within GFF3512 at 18.484 kb on - strand, within GFF3512 at 18.504 kb on + strand, within GFF3512 at 18.504 kb on + strand, within GFF3512 at 18.573 kb on + strand at 18.574 kb on - strand at 18.849 kb on + strand, within GFF3514 at 18.850 kb on - strand, within GFF3514 at 18.888 kb on + strand, within GFF3514 at 18.888 kb on + strand, within GFF3514 at 18.889 kb on - strand, within GFF3514 at 18.889 kb on - strand, within GFF3514 at 19.066 kb on + strand at 19.066 kb on + strand at 19.115 kb on - strand, within GFF3515 at 19.115 kb on - strand, within GFF3515 at 19.162 kb on + strand, within GFF3515 at 19.163 kb on - strand, within GFF3515 at 19.282 kb on + strand, within GFF3515 at 19.283 kb on - strand, within GFF3515 at 19.283 kb on - strand, within GFF3515 at 19.306 kb on + strand, within GFF3515 at 19.307 kb on - strand, within GFF3515 at 19.537 kb on + strand, within GFF3515 at 19.537 kb on + strand, within GFF3515 at 19.537 kb on + strand, within GFF3515 at 19.538 kb on - strand, within GFF3515 at 19.538 kb on - strand, within GFF3515 at 19.538 kb on - strand, within GFF3515 at 19.753 kb on + strand, within GFF3515
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 17,843 + GFF3511 0.68 -0.7 17,965 + GFF3511 0.80 -2.4 17,965 + GFF3511 0.80 -2.5 17,965 + GFF3511 0.80 -0.5 17,966 - GFF3511 0.80 -1.1 17,966 - GFF3511 0.80 -0.1 18,140 + +1.6 18,140 + -0.2 18,149 + -0.5 18,159 - +1.9 18,484 - GFF3512 0.73 -0.2 18,484 - GFF3512 0.73 -1.9 18,484 - GFF3512 0.73 -0.9 18,484 - GFF3512 0.73 -0.3 18,484 - GFF3512 0.73 -0.8 18,504 + GFF3512 0.79 +0.7 18,504 + GFF3512 0.79 +0.3 18,573 + -0.1 18,574 - +0.8 18,849 + GFF3514 0.21 -0.3 18,850 - GFF3514 0.22 +0.4 18,888 + GFF3514 0.61 -3.1 18,888 + GFF3514 0.61 +0.2 18,889 - GFF3514 0.62 +0.4 18,889 - GFF3514 0.62 -0.6 19,066 + -0.4 19,066 + +0.8 19,115 - GFF3515 0.10 -0.9 19,115 - GFF3515 0.10 -2.6 19,162 + GFF3515 0.13 -0.4 19,163 - GFF3515 0.13 +0.2 19,282 + GFF3515 0.20 -0.8 19,283 - GFF3515 0.20 -0.2 19,283 - GFF3515 0.20 -1.5 19,306 + GFF3515 0.21 -0.6 19,307 - GFF3515 0.21 -0.5 19,537 + GFF3515 0.34 -0.5 19,537 + GFF3515 0.34 +0.7 19,537 + GFF3515 0.34 -0.4 19,538 - GFF3515 0.34 -1.2 19,538 - GFF3515 0.34 -0.4 19,538 - GFF3515 0.34 -0.1 19,753 + GFF3515 0.46 -1.6
Or see this region's nucleotide sequence