Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3459 and GFF3460 are separated by 39 nucleotides GFF3460 and GFF3461 are separated by 18 nucleotides GFF3461 and GFF3462 overlap by 4 nucleotides
GFF3459 - Aliphatic amidase AmiE (EC 3.5.1.4), at 281,209 to 282,246
GFF3459
GFF3460 - Urea ABC transporter, ATPase protein UrtE, at 282,286 to 282,975
GFF3460
GFF3461 - Urea ABC transporter, ATPase protein UrtD, at 282,994 to 283,794
GFF3461
GFF3462 - Urea ABC transporter, permease protein UrtC, at 283,791 to 284,846
GFF3462
Position (kb) 282
283 Strain fitness (log2 ratio) -1
0
1 at 281.483 kb on + strand, within GFF3459 at 281.562 kb on - strand, within GFF3459 at 281.562 kb on - strand, within GFF3459 at 281.607 kb on - strand, within GFF3459 at 281.666 kb on + strand, within GFF3459 at 281.673 kb on - strand, within GFF3459 at 281.673 kb on - strand, within GFF3459 at 281.795 kb on + strand, within GFF3459 at 281.796 kb on - strand, within GFF3459 at 281.796 kb on - strand, within GFF3459 at 281.900 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.030 kb on - strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.048 kb on - strand, within GFF3459 at 282.287 kb on + strand at 282.287 kb on + strand at 282.288 kb on - strand at 282.765 kb on - strand, within GFF3460 at 283.007 kb on + strand at 283.008 kb on - strand at 283.897 kb on + strand, within GFF3462 at 283.930 kb on + strand, within GFF3462
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 281,483 + GFF3459 0.26 +0.0 281,562 - GFF3459 0.34 +0.1 281,562 - GFF3459 0.34 -0.7 281,607 - GFF3459 0.38 -0.8 281,666 + GFF3459 0.44 +0.1 281,673 - GFF3459 0.45 -0.1 281,673 - GFF3459 0.45 +0.1 281,795 + GFF3459 0.56 +0.0 281,796 - GFF3459 0.57 -1.3 281,796 - GFF3459 0.57 -0.2 281,900 + GFF3459 0.67 -0.1 282,029 + GFF3459 0.79 -0.6 282,029 + GFF3459 0.79 -1.2 282,029 + GFF3459 0.79 -1.2 282,030 - GFF3459 0.79 +0.4 282,047 + GFF3459 0.81 -0.8 282,047 + GFF3459 0.81 -0.0 282,047 + GFF3459 0.81 +0.5 282,047 + GFF3459 0.81 -0.1 282,048 - GFF3459 0.81 -0.7 282,287 + +0.2 282,287 + -1.2 282,288 - +1.0 282,765 - GFF3460 0.69 +0.5 283,007 + -0.9 283,008 - +0.2 283,897 + GFF3462 0.10 -1.1 283,930 + GFF3462 0.13 +0.4
Or see this region's nucleotide sequence