Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3437 and GFF3438 are separated by 149 nucleotides GFF3438 and GFF3439 are separated by 7 nucleotides
GFF3437 - putative membrane protein, at 259,560 to 260,903
GFF3437
GFF3438 - Amidohydrolase family protein, at 261,053 to 262,222
GFF3438
GFF3439 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 262,230 to 263,612
GFF3439
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.055 kb on + strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.056 kb on - strand, within GFF3437 at 260.097 kb on + strand, within GFF3437 at 260.097 kb on + strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.098 kb on - strand, within GFF3437 at 260.328 kb on + strand, within GFF3437 at 260.329 kb on - strand, within GFF3437 at 260.329 kb on - strand, within GFF3437 at 260.370 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.547 kb on + strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.548 kb on - strand, within GFF3437 at 260.869 kb on + strand at 260.870 kb on - strand at 261.057 kb on - strand at 261.057 kb on - strand at 261.057 kb on - strand at 261.466 kb on - strand, within GFF3438 at 261.778 kb on + strand, within GFF3438 at 262.085 kb on + strand, within GFF3438 at 262.085 kb on + strand, within GFF3438 at 262.086 kb on - strand, within GFF3438 at 262.139 kb on + strand at 262.139 kb on + strand at 262.142 kb on + strand at 262.143 kb on - strand at 262.181 kb on + strand at 262.251 kb on + strand at 262.251 kb on + strand at 262.659 kb on + strand, within GFF3439 at 262.660 kb on - strand, within GFF3439 at 262.660 kb on - strand, within GFF3439 at 262.660 kb on - strand, within GFF3439 at 263.142 kb on + strand, within GFF3439 at 263.142 kb on + strand, within GFF3439 at 263.142 kb on + strand, within GFF3439 at 263.143 kb on - strand, within GFF3439 at 263.143 kb on - strand, within GFF3439 at 263.145 kb on + strand, within GFF3439 at 263.146 kb on - strand, within GFF3439
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 260,055 + GFF3437 0.37 +0.6 260,055 + GFF3437 0.37 -0.3 260,055 + GFF3437 0.37 +0.7 260,055 + GFF3437 0.37 -0.8 260,056 - GFF3437 0.37 -0.8 260,056 - GFF3437 0.37 +0.7 260,056 - GFF3437 0.37 -1.0 260,097 + GFF3437 0.40 +0.4 260,097 + GFF3437 0.40 -0.8 260,098 - GFF3437 0.40 -0.1 260,098 - GFF3437 0.40 -0.8 260,098 - GFF3437 0.40 -0.2 260,098 - GFF3437 0.40 +0.4 260,098 - GFF3437 0.40 +0.3 260,328 + GFF3437 0.57 +0.0 260,329 - GFF3437 0.57 -0.2 260,329 - GFF3437 0.57 +0.3 260,370 + GFF3437 0.60 -0.2 260,547 + GFF3437 0.73 -2.1 260,547 + GFF3437 0.73 +1.0 260,547 + GFF3437 0.73 -0.3 260,547 + GFF3437 0.73 +0.2 260,547 + GFF3437 0.73 -0.5 260,548 - GFF3437 0.74 +0.4 260,548 - GFF3437 0.74 -3.2 260,548 - GFF3437 0.74 +1.0 260,548 - GFF3437 0.74 +0.7 260,548 - GFF3437 0.74 -0.9 260,869 + +0.5 260,870 - +1.6 261,057 - -0.1 261,057 - +0.0 261,057 - -1.3 261,466 - GFF3438 0.35 -0.8 261,778 + GFF3438 0.62 -1.3 262,085 + GFF3438 0.88 -1.9 262,085 + GFF3438 0.88 -1.5 262,086 - GFF3438 0.88 +1.0 262,139 + +0.6 262,139 + -1.4 262,142 + -0.4 262,143 - -0.3 262,181 + -0.6 262,251 + +0.7 262,251 + -0.1 262,659 + GFF3439 0.31 -0.5 262,660 - GFF3439 0.31 +0.3 262,660 - GFF3439 0.31 +0.0 262,660 - GFF3439 0.31 +1.7 263,142 + GFF3439 0.66 -0.1 263,142 + GFF3439 0.66 +0.0 263,142 + GFF3439 0.66 -0.3 263,143 - GFF3439 0.66 -0.2 263,143 - GFF3439 0.66 -0.7 263,145 + GFF3439 0.66 -0.0 263,146 - GFF3439 0.66 -0.5
Or see this region's nucleotide sequence