Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3433 and GFF3434 are separated by 188 nucleotides GFF3434 and GFF3435 are separated by 1 nucleotides GFF3435 and GFF3436 are separated by 67 nucleotides
GFF3433 - hypothetical protein, at 256,903 to 257,334
GFF3433
GFF3434 - Organic hydroperoxide resistance protein, at 257,523 to 257,936
GFF3434
GFF3435 - predicted integral membrane protein, at 257,938 to 258,396
GFF3435
GFF3436 - Transcriptional regulator, LysR family, at 258,464 to 259,432
GFF3436
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1 at 256.993 kb on + strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.279 kb on - strand, within GFF3433 at 257.333 kb on - strand at 257.370 kb on + strand at 257.370 kb on + strand at 257.371 kb on - strand at 257.371 kb on - strand at 257.371 kb on - strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.399 kb on + strand at 257.400 kb on - strand at 257.400 kb on - strand at 257.442 kb on + strand at 257.442 kb on + strand at 257.457 kb on + strand at 257.457 kb on + strand at 257.458 kb on - strand at 257.458 kb on - strand at 257.544 kb on + strand at 257.544 kb on + strand at 257.545 kb on - strand at 257.793 kb on + strand, within GFF3434 at 257.793 kb on + strand, within GFF3434 at 257.793 kb on + strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.794 kb on - strand, within GFF3434 at 257.886 kb on + strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 257.887 kb on - strand, within GFF3434 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.098 kb on + strand, within GFF3435 at 258.099 kb on - strand, within GFF3435 at 258.099 kb on - strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.104 kb on + strand, within GFF3435 at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.390 kb on - strand at 258.529 kb on - strand at 258.708 kb on + strand, within GFF3436 at 258.708 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.119 kb on + strand, within GFF3436 at 259.120 kb on - strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.221 kb on + strand, within GFF3436 at 259.222 kb on - strand, within GFF3436
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 256,993 + GFF3433 0.21 -1.5 257,279 - GFF3433 0.87 -0.4 257,279 - GFF3433 0.87 -0.1 257,279 - GFF3433 0.87 -0.6 257,279 - GFF3433 0.87 +0.2 257,333 - -0.3 257,370 + +0.0 257,370 + -0.3 257,371 - -0.6 257,371 - -0.6 257,371 - -0.6 257,399 + +0.6 257,399 + -0.4 257,399 + +1.7 257,399 + -1.5 257,399 + -1.3 257,399 + -2.0 257,399 + +1.4 257,399 + -0.4 257,400 - +0.5 257,400 - -0.4 257,442 + +1.0 257,442 + -0.1 257,457 + -0.2 257,457 + +1.0 257,458 - +1.4 257,458 - -0.5 257,544 + -1.5 257,544 + +0.0 257,545 - -0.2 257,793 + GFF3434 0.65 -0.1 257,793 + GFF3434 0.65 -0.1 257,793 + GFF3434 0.65 -0.7 257,794 - GFF3434 0.65 -0.4 257,794 - GFF3434 0.65 +0.2 257,794 - GFF3434 0.65 +0.2 257,886 + GFF3434 0.88 +0.1 257,887 - GFF3434 0.88 +0.4 257,887 - GFF3434 0.88 +0.8 257,887 - GFF3434 0.88 -0.9 258,098 + GFF3435 0.35 -1.2 258,098 + GFF3435 0.35 -0.1 258,098 + GFF3435 0.35 +0.1 258,098 + GFF3435 0.35 +0.5 258,098 + GFF3435 0.35 +1.4 258,099 - GFF3435 0.35 +0.6 258,099 - GFF3435 0.35 +0.2 258,104 + GFF3435 0.36 -0.4 258,104 + GFF3435 0.36 +0.4 258,104 + GFF3435 0.36 +1.5 258,104 + GFF3435 0.36 +1.0 258,104 + GFF3435 0.36 -0.6 258,104 + GFF3435 0.36 +1.0 258,104 + GFF3435 0.36 +0.2 258,390 - -0.2 258,390 - +0.0 258,390 - +0.3 258,390 - +0.1 258,390 - +0.1 258,390 - +0.5 258,529 - -0.1 258,708 + GFF3436 0.25 +0.4 258,708 + GFF3436 0.25 -1.7 259,119 + GFF3436 0.68 -0.5 259,119 + GFF3436 0.68 -0.4 259,120 - GFF3436 0.68 -1.2 259,221 + GFF3436 0.78 +0.3 259,221 + GFF3436 0.78 -0.4 259,222 - GFF3436 0.78 +0.7
Or see this region's nucleotide sequence