Strain Fitness in Variovorax sp. SCN45 around GFF3263

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
82,197	+		GFF3261	0.61	+1.4
82,198	-		GFF3261	0.61	+0.4
82,198	-		GFF3261	0.61	-0.8
82,198	-		GFF3261	0.61	-0.3
82,198	-		GFF3261	0.61	-0.5
82,198	-		GFF3261	0.61	+0.5
82,198	-		GFF3261	0.61	+1.2
82,332	+		GFF3261	0.78	+0.7
82,333	-		GFF3261	0.79	-0.8
82,333	-		GFF3261	0.79	+0.1
82,413	+		GFF3261	0.89	+0.4
82,413	+		GFF3261	0.89	-0.3
82,413	+		GFF3261	0.89	-0.7
82,601	-		GFF3262	0.14	+0.8
82,698	+		GFF3262	0.33	-0.8
82,698	+		GFF3262	0.33	-0.1
82,699	-		GFF3262	0.33	-1.2
82,699	-		GFF3262	0.33	-0.3
82,699	-		GFF3262	0.33	-0.8
82,699	-		GFF3262	0.33	+0.1
82,699	-		GFF3262	0.33	+1.0
83,027	+				-0.4
83,027	+				-0.7
83,028	-				-1.3
83,028	-				-0.7
83,060	-				+0.1
83,060	-				-1.2
83,064	+				+0.4
83,064	+				-0.7
83,064	+				+0.4
83,064	+				+0.1
83,065	-				+0.4
83,065	-				-1.1
83,065	-				-0.7
83,065	-				-0.9
83,117	+				-0.6
83,118	-				-2.9
83,118	-				-1.5
83,416	+		GFF3263	0.29	-1.0
83,416	+		GFF3263	0.29	+0.6
83,416	+		GFF3263	0.29	-0.6
83,416	+		GFF3263	0.29	+0.3
83,416	+		GFF3263	0.29	-0.8
83,416	+		GFF3263	0.29	+0.4
83,417	-		GFF3263	0.29	-1.2
83,417	-		GFF3263	0.29	-0.1
83,417	-		GFF3263	0.29	-0.7
83,417	-		GFF3263	0.29	-0.9
83,417	-		GFF3263	0.29	-0.4
83,417	-		GFF3263	0.29	-0.6
83,455	+		GFF3263	0.34	-1.3
83,455	+		GFF3263	0.34	-0.1
83,470	+		GFF3263	0.35	-0.2
83,471	-		GFF3263	0.35	-0.4
83,471	-		GFF3263	0.35	+1.0
83,536	+		GFF3263	0.42	-0.6
83,536	+		GFF3263	0.42	-0.3
83,537	-		GFF3263	0.42	-0.2
83,537	-		GFF3263	0.42	+0.7
83,611	+		GFF3263	0.50	+1.9
83,710	+		GFF3263	0.61	-0.4
83,710	+		GFF3263	0.61	-0.2
83,710	+		GFF3263	0.61	-0.5
83,710	+		GFF3263	0.61	+0.6
83,710	+		GFF3263	0.61	-0.8
83,710	+		GFF3263	0.61	-0.4
83,710	+		GFF3263	0.61	-0.8
83,710	+		GFF3263	0.61	-1.5
83,710	+		GFF3263	0.61	+1.4
83,710	+		GFF3263	0.61	-0.6
83,710	+		GFF3263	0.61	+0.1
83,710	+		GFF3263	0.61	-0.7
83,710	+		GFF3263	0.61	-0.3
83,710	+		GFF3263	0.61	-0.7
83,711	-		GFF3263	0.61	-0.7
83,711	-		GFF3263	0.61	-0.1
83,711	-		GFF3263	0.61	-1.2
83,711	-		GFF3263	0.61	+0.7
83,711	-		GFF3263	0.61	-0.9
83,711	-		GFF3263	0.61	-1.3
83,711	-		GFF3263	0.61	+0.3
83,711	-		GFF3263	0.61	+0.9
83,711	-		GFF3263	0.61	-1.0
83,761	+		GFF3263	0.67	+0.3
83,762	-		GFF3263	0.67	-0.3
83,762	-		GFF3263	0.67	+0.0
84,270	+				+1.2
84,271	-				+0.0
84,271	-				-0.3
84,271	-				+0.3
84,271	-				+0.2
84,271	-				-0.7
84,271	-				+0.5
84,271	-				-0.8
84,315	+				-0.5
84,315	+				+0.7
84,316	-				+1.0
84,316	-				-1.5
84,801	+		GFF3264	0.29	-0.2
84,801	+		GFF3264	0.29	-0.6
84,801	+		GFF3264	0.29	+0.4
84,801	+		GFF3264	0.29	-1.1
84,801	+		GFF3264	0.29	-0.6
84,802	-		GFF3264	0.29	-0.7
84,802	-		GFF3264	0.29	-2.5
84,802	-		GFF3264	0.29	-1.9
84,802	-		GFF3264	0.29	+0.4
84,802	-		GFF3264	0.29	-1.7
84,811	-		GFF3264	0.31	-1.8