Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF30 and GFF31 overlap by 4 nucleotides GFF31 and GFF32 overlap by 4 nucleotides GFF32 and GFF33 overlap by 4 nucleotides GFF33 and GFF34 are separated by 87 nucleotides
GFF30 - Murein endolytic transglycosylase MltG, at 34,445 to 35,467
GFF30
GFF31 - Thymidylate kinase (EC 2.7.4.9), at 35,464 to 36,135
GFF31
GFF32 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 36,132 to 37,145
GFF32
GFF33 - Type IV pilus biogenesis protein PilZ, at 37,142 to 37,528
GFF33
GFF34 - Uncharacterized metal-dependent hydrolase YcfH, at 37,616 to 38,422
GFF34
Position (kb) 36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 35.183 kb on + strand, within GFF30 at 35.183 kb on + strand, within GFF30 at 35.183 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.255 kb on + strand, within GFF30 at 35.348 kb on + strand, within GFF30 at 37.186 kb on - strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.238 kb on + strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.239 kb on - strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.247 kb on + strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.248 kb on - strand, within GFF33 at 37.298 kb on + strand, within GFF33 at 37.299 kb on - strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.319 kb on + strand, within GFF33 at 37.320 kb on - strand, within GFF33 at 37.320 kb on - strand, within GFF33 at 37.320 kb on - strand, within GFF33 at 37.320 kb on - strand, within GFF33 at 37.320 kb on - strand, within GFF33 at 37.561 kb on + strand at 37.561 kb on + strand at 37.561 kb on + strand at 37.562 kb on - strand at 37.562 kb on - strand at 37.772 kb on + strand, within GFF34 at 37.772 kb on + strand, within GFF34 at 37.772 kb on + strand, within GFF34 at 37.772 kb on + strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.773 kb on - strand, within GFF34 at 37.774 kb on - strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.901 kb on + strand, within GFF34 at 37.902 kb on - strand, within GFF34 at 37.902 kb on - strand, within GFF34 at 37.902 kb on - strand, within GFF34 at 37.904 kb on + strand, within GFF34 at 37.904 kb on + strand, within GFF34 at 37.904 kb on + strand, within GFF34 at 37.905 kb on - strand, within GFF34
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 35,183 + GFF30 0.72 -0.6 35,183 + GFF30 0.72 -0.8 35,183 + GFF30 0.72 +0.2 35,255 + GFF30 0.79 +0.7 35,255 + GFF30 0.79 -0.4 35,255 + GFF30 0.79 +0.7 35,255 + GFF30 0.79 -0.2 35,255 + GFF30 0.79 -1.2 35,255 + GFF30 0.79 +0.5 35,255 + GFF30 0.79 -0.6 35,255 + GFF30 0.79 -1.4 35,255 + GFF30 0.79 -1.3 35,255 + GFF30 0.79 -0.9 35,348 + GFF30 0.88 -0.4 37,186 - GFF33 0.11 -1.2 37,238 + GFF33 0.25 -0.8 37,238 + GFF33 0.25 -1.5 37,238 + GFF33 0.25 -2.4 37,238 + GFF33 0.25 -1.4 37,238 + GFF33 0.25 -1.3 37,238 + GFF33 0.25 -0.4 37,238 + GFF33 0.25 -0.8 37,239 - GFF33 0.25 -2.1 37,239 - GFF33 0.25 -0.8 37,239 - GFF33 0.25 -0.4 37,239 - GFF33 0.25 -0.4 37,239 - GFF33 0.25 -3.5 37,239 - GFF33 0.25 -0.2 37,239 - GFF33 0.25 -2.2 37,247 + GFF33 0.27 -0.9 37,247 + GFF33 0.27 -1.1 37,247 + GFF33 0.27 -0.8 37,247 + GFF33 0.27 -1.2 37,247 + GFF33 0.27 +1.6 37,247 + GFF33 0.27 -0.7 37,247 + GFF33 0.27 -1.2 37,247 + GFF33 0.27 -0.9 37,248 - GFF33 0.27 -0.8 37,248 - GFF33 0.27 -1.3 37,248 - GFF33 0.27 -1.7 37,248 - GFF33 0.27 -1.0 37,248 - GFF33 0.27 -2.5 37,248 - GFF33 0.27 -2.5 37,248 - GFF33 0.27 -0.6 37,298 + GFF33 0.40 -2.1 37,299 - GFF33 0.41 -1.5 37,319 + GFF33 0.46 -0.9 37,319 + GFF33 0.46 -1.2 37,319 + GFF33 0.46 -2.3 37,319 + GFF33 0.46 -1.5 37,319 + GFF33 0.46 -1.7 37,319 + GFF33 0.46 -2.1 37,319 + GFF33 0.46 -1.7 37,319 + GFF33 0.46 -1.4 37,319 + GFF33 0.46 -0.7 37,320 - GFF33 0.46 -1.7 37,320 - GFF33 0.46 -1.0 37,320 - GFF33 0.46 -2.1 37,320 - GFF33 0.46 +0.3 37,320 - GFF33 0.46 -0.8 37,561 + -0.1 37,561 + -0.0 37,561 + -0.9 37,562 - -0.3 37,562 - -0.1 37,772 + GFF34 0.19 -1.0 37,772 + GFF34 0.19 +0.1 37,772 + GFF34 0.19 -0.2 37,772 + GFF34 0.19 -1.0 37,773 - GFF34 0.19 -0.5 37,773 - GFF34 0.19 -1.2 37,773 - GFF34 0.19 +1.6 37,773 - GFF34 0.19 +1.1 37,773 - GFF34 0.19 -2.0 37,773 - GFF34 0.19 +0.5 37,774 - GFF34 0.20 -0.4 37,901 + GFF34 0.35 -1.3 37,901 + GFF34 0.35 -0.8 37,901 + GFF34 0.35 -0.1 37,901 + GFF34 0.35 +0.7 37,901 + GFF34 0.35 -0.6 37,901 + GFF34 0.35 -0.6 37,902 - GFF34 0.35 +0.4 37,902 - GFF34 0.35 -0.9 37,902 - GFF34 0.35 +0.1 37,904 + GFF34 0.36 +0.6 37,904 + GFF34 0.36 +0.4 37,904 + GFF34 0.36 -0.3 37,905 - GFF34 0.36 -1.3
Or see this region's nucleotide sequence