Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3180 and GFF3181 are separated by 8 nucleotides GFF3181 and GFF3182 overlap by 4 nucleotides GFF3182 and GFF3183 are separated by 33 nucleotides GFF3183 and GFF3184 are separated by 70 nucleotides
GFF3180 - FIG00348182: hypothetical protein, at 622,660 to 623,316
GFF3180
GFF3181 - FIG005069: Hypothetical protein, at 623,325 to 624,128
GFF3181
GFF3182 - Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069, at 624,125 to 625,171
GFF3182
GFF3183 - no description, at 625,205 to 625,480
GFF3183
GFF3184 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 625,551 to 627,512
GFF3184
Position (kb)
624
625
626 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 623.389 kb on + strand at 623.390 kb on - strand at 623.390 kb on - strand at 623.566 kb on + strand, within GFF3181 at 623.566 kb on + strand, within GFF3181 at 623.566 kb on + strand, within GFF3181 at 623.584 kb on + strand, within GFF3181 at 623.585 kb on - strand, within GFF3181 at 623.803 kb on + strand, within GFF3181 at 623.803 kb on + strand, within GFF3181 at 623.804 kb on - strand, within GFF3181 at 623.804 kb on - strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.896 kb on + strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.897 kb on - strand, within GFF3181 at 623.981 kb on - strand, within GFF3181 at 623.981 kb on - strand, within GFF3181 at 623.981 kb on - strand, within GFF3181 at 624.004 kb on + strand, within GFF3181 at 624.004 kb on + strand, within GFF3181 at 624.005 kb on - strand, within GFF3181 at 624.255 kb on + strand, within GFF3182 at 624.255 kb on + strand, within GFF3182 at 624.333 kb on + strand, within GFF3182 at 624.432 kb on + strand, within GFF3182 at 624.433 kb on - strand, within GFF3182 at 624.433 kb on - strand, within GFF3182 at 624.433 kb on - strand, within GFF3182 at 624.433 kb on - strand, within GFF3182 at 624.694 kb on - strand, within GFF3182 at 624.904 kb on - strand, within GFF3182 at 624.954 kb on + strand, within GFF3182 at 624.954 kb on + strand, within GFF3182 at 624.955 kb on - strand, within GFF3182 at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.468 kb on - strand at 625.471 kb on + strand at 625.471 kb on + strand at 625.471 kb on + strand at 625.472 kb on - strand at 625.472 kb on - strand at 625.472 kb on - strand at 625.472 kb on - strand at 625.704 kb on + strand at 625.767 kb on + strand, within GFF3184 at 625.767 kb on + strand, within GFF3184 at 625.768 kb on - strand, within GFF3184 at 625.768 kb on - strand, within GFF3184 at 625.884 kb on + strand, within GFF3184 at 625.884 kb on + strand, within GFF3184 at 625.885 kb on - strand, within GFF3184 at 625.908 kb on + strand, within GFF3184 at 625.909 kb on - strand, within GFF3184 at 626.034 kb on + strand, within GFF3184 at 626.034 kb on + strand, within GFF3184 at 626.035 kb on - strand, within GFF3184
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 623,389 + +0.1 623,390 - -0.1 623,390 - -0.5 623,566 + GFF3181 0.30 -0.1 623,566 + GFF3181 0.30 +0.3 623,566 + GFF3181 0.30 -0.2 623,584 + GFF3181 0.32 +0.5 623,585 - GFF3181 0.32 -1.7 623,803 + GFF3181 0.59 +0.1 623,803 + GFF3181 0.59 +0.1 623,804 - GFF3181 0.60 +0.4 623,804 - GFF3181 0.60 -0.1 623,896 + GFF3181 0.71 -0.4 623,896 + GFF3181 0.71 -1.1 623,896 + GFF3181 0.71 -0.4 623,896 + GFF3181 0.71 -0.5 623,896 + GFF3181 0.71 +0.2 623,896 + GFF3181 0.71 +0.2 623,896 + GFF3181 0.71 -0.7 623,896 + GFF3181 0.71 -0.7 623,897 - GFF3181 0.71 +2.0 623,897 - GFF3181 0.71 -0.7 623,897 - GFF3181 0.71 -0.3 623,897 - GFF3181 0.71 -0.1 623,897 - GFF3181 0.71 -1.3 623,897 - GFF3181 0.71 +0.3 623,897 - GFF3181 0.71 -0.3 623,897 - GFF3181 0.71 -0.5 623,897 - GFF3181 0.71 -0.6 623,981 - GFF3181 0.82 -0.9 623,981 - GFF3181 0.82 +0.4 623,981 - GFF3181 0.82 -0.3 624,004 + GFF3181 0.84 -0.1 624,004 + GFF3181 0.84 -0.0 624,005 - GFF3181 0.85 -0.9 624,255 + GFF3182 0.12 -0.4 624,255 + GFF3182 0.12 -1.1 624,333 + GFF3182 0.20 -1.2 624,432 + GFF3182 0.29 +0.7 624,433 - GFF3182 0.29 -0.6 624,433 - GFF3182 0.29 -1.2 624,433 - GFF3182 0.29 -1.9 624,433 - GFF3182 0.29 -0.9 624,694 - GFF3182 0.54 +0.6 624,904 - GFF3182 0.74 -0.4 624,954 + GFF3182 0.79 +0.4 624,954 + GFF3182 0.79 -0.6 624,955 - GFF3182 0.79 +0.2 625,467 + -0.3 625,467 + -0.5 625,467 + +0.8 625,467 + -0.2 625,467 + -0.3 625,467 + -0.3 625,467 + +0.4 625,468 - -0.0 625,468 - -0.6 625,468 - -0.5 625,468 - -0.6 625,468 - -0.8 625,468 - +1.1 625,468 - -0.1 625,468 - +0.7 625,468 - -1.0 625,468 - -0.6 625,468 - -0.3 625,471 + -0.9 625,471 + -0.3 625,471 + +0.7 625,472 - +0.8 625,472 - -0.5 625,472 - +0.4 625,472 - -0.5 625,704 + +0.4 625,767 + GFF3184 0.11 -0.9 625,767 + GFF3184 0.11 +1.0 625,768 - GFF3184 0.11 -0.6 625,768 - GFF3184 0.11 -0.6 625,884 + GFF3184 0.17 -0.3 625,884 + GFF3184 0.17 +0.2 625,885 - GFF3184 0.17 -1.1 625,908 + GFF3184 0.18 -0.8 625,909 - GFF3184 0.18 -1.2 626,034 + GFF3184 0.25 +0.0 626,034 + GFF3184 0.25 -0.6 626,035 - GFF3184 0.25 -1.2
Or see this region's nucleotide sequence