Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3124 and GFF3125 are separated by 4 nucleotides GFF3125 and GFF3126 are separated by 63 nucleotides GFF3126 and GFF3127 are separated by 149 nucleotides GFF3127 and GFF3128 overlap by 4 nucleotides
GFF3124 - no description, at 552,336 to 552,674
GFF3124
GFF3125 - no description, at 552,679 to 553,125
GFF3125
GFF3126 - no description, at 553,189 to 553,476
GFF3126
GFF3127 - no description, at 553,626 to 554,162
GFF3127
GFF3128 - T6SS PAAR-repeat protein / RhaS protein, at 554,159 to 558,616
GFF3128
Position (kb) 553
554
555 Strain fitness (log2 ratio) -2
-1
0
1
2 at 552.699 kb on - strand at 552.881 kb on + strand, within GFF3125 at 552.920 kb on + strand, within GFF3125 at 552.921 kb on - strand, within GFF3125 at 552.921 kb on - strand, within GFF3125 at 552.921 kb on - strand, within GFF3125 at 552.939 kb on - strand, within GFF3125 at 553.279 kb on - strand, within GFF3126 at 553.365 kb on - strand, within GFF3126 at 553.502 kb on + strand at 553.502 kb on + strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.503 kb on - strand at 553.618 kb on + strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.657 kb on + strand at 553.658 kb on - strand at 553.658 kb on - strand at 553.658 kb on - strand at 553.678 kb on + strand at 553.678 kb on + strand at 553.679 kb on - strand at 553.679 kb on - strand at 553.679 kb on - strand at 553.680 kb on + strand, within GFF3127 at 553.680 kb on + strand, within GFF3127 at 553.680 kb on + strand, within GFF3127 at 553.758 kb on + strand, within GFF3127 at 553.758 kb on + strand, within GFF3127 at 553.783 kb on + strand, within GFF3127 at 553.843 kb on - strand, within GFF3127 at 553.843 kb on - strand, within GFF3127 at 553.876 kb on + strand, within GFF3127 at 553.877 kb on - strand, within GFF3127 at 553.916 kb on + strand, within GFF3127 at 553.945 kb on - strand, within GFF3127 at 554.300 kb on + strand at 554.300 kb on + strand at 554.300 kb on + strand at 554.301 kb on - strand at 554.302 kb on + strand at 554.347 kb on - strand at 554.444 kb on + strand at 554.444 kb on + strand at 554.445 kb on - strand at 554.506 kb on - strand at 554.551 kb on - strand at 554.551 kb on - strand at 554.622 kb on + strand, within GFF3128 at 554.623 kb on - strand, within GFF3128 at 554.623 kb on - strand, within GFF3128 at 554.640 kb on + strand, within GFF3128 at 554.640 kb on + strand, within GFF3128 at 554.641 kb on - strand, within GFF3128 at 554.641 kb on - strand, within GFF3128 at 554.641 kb on - strand, within GFF3128 at 554.641 kb on - strand, within GFF3128 at 554.641 kb on - strand, within GFF3128 at 554.668 kb on - strand, within GFF3128 at 554.790 kb on + strand, within GFF3128 at 554.791 kb on - strand, within GFF3128 at 554.791 kb on - strand, within GFF3128 at 554.949 kb on + strand, within GFF3128 at 554.950 kb on - strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.955 kb on + strand, within GFF3128 at 554.956 kb on - strand, within GFF3128 at 554.956 kb on - strand, within GFF3128
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 552,699 - -1.9 552,881 + GFF3125 0.45 -2.0 552,920 + GFF3125 0.54 -1.7 552,921 - GFF3125 0.54 +0.7 552,921 - GFF3125 0.54 -0.7 552,921 - GFF3125 0.54 -1.0 552,939 - GFF3125 0.58 -0.3 553,279 - GFF3126 0.31 -0.9 553,365 - GFF3126 0.61 -2.4 553,502 + +0.7 553,502 + +0.6 553,503 - -0.4 553,503 - +1.0 553,503 - -0.1 553,503 - +0.8 553,503 - -0.7 553,503 - -0.0 553,503 - +0.3 553,503 - +0.5 553,618 + -0.6 553,657 + -0.3 553,657 + -0.8 553,657 + -0.1 553,658 - -0.4 553,658 - +0.7 553,658 - -0.3 553,678 + -0.0 553,678 + -0.4 553,679 - +1.7 553,679 - +0.9 553,679 - +0.4 553,680 + GFF3127 0.10 -0.3 553,680 + GFF3127 0.10 -0.5 553,680 + GFF3127 0.10 +1.2 553,758 + GFF3127 0.25 -0.2 553,758 + GFF3127 0.25 -0.6 553,783 + GFF3127 0.29 +0.6 553,843 - GFF3127 0.40 +0.1 553,843 - GFF3127 0.40 -0.6 553,876 + GFF3127 0.47 +0.1 553,877 - GFF3127 0.47 +2.3 553,916 + GFF3127 0.54 -0.2 553,945 - GFF3127 0.59 +1.0 554,300 + -0.9 554,300 + +0.3 554,300 + -1.4 554,301 - -0.6 554,302 + +0.0 554,347 - -0.6 554,444 + +0.3 554,444 + -0.4 554,445 - +0.3 554,506 - +0.8 554,551 - +0.7 554,551 - +0.7 554,622 + GFF3128 0.10 -0.3 554,623 - GFF3128 0.10 -0.3 554,623 - GFF3128 0.10 -0.9 554,640 + GFF3128 0.11 -2.5 554,640 + GFF3128 0.11 -0.8 554,641 - GFF3128 0.11 +1.1 554,641 - GFF3128 0.11 +0.5 554,641 - GFF3128 0.11 -0.4 554,641 - GFF3128 0.11 -0.6 554,641 - GFF3128 0.11 -0.0 554,668 - GFF3128 0.11 -0.2 554,790 + GFF3128 0.14 -1.4 554,791 - GFF3128 0.14 -0.3 554,791 - GFF3128 0.14 +1.1 554,949 + GFF3128 0.18 -0.1 554,950 - GFF3128 0.18 +0.7 554,955 + GFF3128 0.18 +0.1 554,955 + GFF3128 0.18 -0.3 554,955 + GFF3128 0.18 -0.1 554,955 + GFF3128 0.18 -0.1 554,955 + GFF3128 0.18 -0.5 554,955 + GFF3128 0.18 -0.3 554,955 + GFF3128 0.18 -0.7 554,955 + GFF3128 0.18 +0.0 554,956 - GFF3128 0.18 -1.2 554,956 - GFF3128 0.18 +0.3
Or see this region's nucleotide sequence