Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2941 and GFF2942 are separated by 21 nucleotides GFF2942 and GFF2943 overlap by 4 nucleotides GFF2943 and GFF2944 are separated by 39 nucleotides GFF2944 and GFF2945 are separated by 23 nucleotides GFF2945 and GFF2946 overlap by 4 nucleotides
GFF2941 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 367,511 to 368,617
GFF2941
GFF2942 - Epoxide hydrolase (EC 3.3.2.9), at 368,639 to 369,559
GFF2942
GFF2943 - S-formylglutathione hydrolase (EC 3.1.2.12), at 369,556 to 370,434
GFF2943
GFF2944 - Acetyltransferase, at 370,474 to 370,977
GFF2944
GFF2945 - Possible glyoxylase family protein (Lactoylglutathione lyase) (EC 4.4.1.5), at 371,001 to 371,390
GFF2945
GFF2946 - Regulator of competence-specific genes, at 371,387 to 371,797
GFF2946
Position (kb) 369
370
371 Strain fitness (log2 ratio) -2
-1
0
1
2 at 368.612 kb on + strand at 368.612 kb on + strand at 368.612 kb on + strand at 368.613 kb on - strand at 368.613 kb on - strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.694 kb on - strand at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.819 kb on + strand, within GFF2942 at 368.819 kb on + strand, within GFF2942 at 368.820 kb on - strand, within GFF2942 at 368.820 kb on - strand, within GFF2942 at 368.831 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.098 kb on + strand, within GFF2942 at 369.099 kb on - strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.667 kb on + strand, within GFF2943 at 369.668 kb on - strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.713 kb on - strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 370.144 kb on + strand, within GFF2943 at 370.145 kb on - strand, within GFF2943 at 370.357 kb on + strand at 370.357 kb on + strand at 370.358 kb on - strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.370 kb on - strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.470 kb on + strand at 370.471 kb on - strand at 370.729 kb on + strand, within GFF2944 at 370.729 kb on + strand, within GFF2944 at 370.729 kb on + strand, within GFF2944 at 370.729 kb on + strand, within GFF2944 at 370.730 kb on - strand, within GFF2944 at 370.730 kb on - strand, within GFF2944 at 370.730 kb on - strand, within GFF2944 at 370.867 kb on + strand, within GFF2944 at 370.868 kb on - strand, within GFF2944 at 370.868 kb on - strand, within GFF2944 at 370.868 kb on - strand, within GFF2944 at 370.868 kb on - strand, within GFF2944 at 370.868 kb on - strand, within GFF2944 at 370.936 kb on + strand at 370.936 kb on + strand at 370.937 kb on - strand at 371.058 kb on + strand, within GFF2945 at 371.058 kb on + strand, within GFF2945 at 371.058 kb on + strand, within GFF2945 at 371.058 kb on + strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.059 kb on - strand, within GFF2945 at 371.067 kb on + strand, within GFF2945 at 371.067 kb on + strand, within GFF2945 at 371.067 kb on + strand, within GFF2945 at 371.068 kb on - strand, within GFF2945 at 371.094 kb on + strand, within GFF2945 at 371.094 kb on + strand, within GFF2945 at 371.094 kb on + strand, within GFF2945 at 371.095 kb on - strand, within GFF2945 at 371.095 kb on - strand, within GFF2945 at 371.095 kb on - strand, within GFF2945 at 371.153 kb on + strand, within GFF2945 at 371.334 kb on + strand, within GFF2945 at 371.334 kb on + strand, within GFF2945 at 371.337 kb on + strand, within GFF2945 at 371.337 kb on + strand, within GFF2945 at 371.337 kb on + strand, within GFF2945 at 371.338 kb on - strand, within GFF2945 at 371.338 kb on - strand, within GFF2945 at 371.338 kb on - strand, within GFF2945
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 368,612 + +1.7 368,612 + +0.2 368,612 + +0.9 368,613 - +0.3 368,613 - -0.1 368,693 + -0.3 368,693 + -1.5 368,693 + -0.8 368,693 + -0.5 368,694 - +0.2 368,765 + GFF2942 0.14 -0.3 368,765 + GFF2942 0.14 -0.3 368,765 + GFF2942 0.14 -0.7 368,765 + GFF2942 0.14 +0.4 368,765 + GFF2942 0.14 -2.5 368,765 + GFF2942 0.14 +1.7 368,765 + GFF2942 0.14 +1.5 368,765 + GFF2942 0.14 -0.4 368,765 + GFF2942 0.14 -0.9 368,765 + GFF2942 0.14 -0.8 368,765 + GFF2942 0.14 -1.5 368,765 + GFF2942 0.14 -1.5 368,766 - GFF2942 0.14 -0.3 368,766 - GFF2942 0.14 +0.6 368,766 - GFF2942 0.14 -1.5 368,766 - GFF2942 0.14 +2.3 368,766 - GFF2942 0.14 +0.4 368,819 + GFF2942 0.20 -0.8 368,819 + GFF2942 0.20 -0.6 368,820 - GFF2942 0.20 -0.9 368,820 - GFF2942 0.20 +0.3 368,831 + GFF2942 0.21 +0.6 369,032 + GFF2942 0.43 -1.5 369,032 + GFF2942 0.43 +0.4 369,032 + GFF2942 0.43 -0.5 369,032 + GFF2942 0.43 +0.7 369,033 - GFF2942 0.43 +0.0 369,033 - GFF2942 0.43 +0.0 369,033 - GFF2942 0.43 -0.1 369,033 - GFF2942 0.43 -0.6 369,033 - GFF2942 0.43 -0.2 369,033 - GFF2942 0.43 -1.3 369,033 - GFF2942 0.43 -0.5 369,098 + GFF2942 0.50 +0.5 369,099 - GFF2942 0.50 +0.2 369,104 + GFF2942 0.50 -1.1 369,104 + GFF2942 0.50 -1.1 369,104 + GFF2942 0.50 +0.4 369,104 + GFF2942 0.50 -0.3 369,104 + GFF2942 0.50 -0.4 369,104 + GFF2942 0.50 -0.1 369,104 + GFF2942 0.50 +0.3 369,104 + GFF2942 0.50 -1.7 369,105 - GFF2942 0.51 +0.4 369,105 - GFF2942 0.51 +0.0 369,105 - GFF2942 0.51 -1.1 369,105 - GFF2942 0.51 -2.0 369,105 - GFF2942 0.51 +0.5 369,105 - GFF2942 0.51 +1.1 369,105 - GFF2942 0.51 +1.0 369,105 - GFF2942 0.51 +1.4 369,105 - GFF2942 0.51 -0.3 369,105 - GFF2942 0.51 +0.3 369,105 - GFF2942 0.51 +0.1 369,105 - GFF2942 0.51 +0.2 369,107 + GFF2942 0.51 -0.1 369,107 + GFF2942 0.51 +0.8 369,107 + GFF2942 0.51 +0.2 369,107 + GFF2942 0.51 -1.2 369,107 + GFF2942 0.51 -0.3 369,107 + GFF2942 0.51 -0.2 369,107 + GFF2942 0.51 +0.7 369,108 - GFF2942 0.51 +0.3 369,108 - GFF2942 0.51 +0.1 369,108 - GFF2942 0.51 +0.1 369,108 - GFF2942 0.51 -0.1 369,108 - GFF2942 0.51 -0.5 369,108 - GFF2942 0.51 +1.0 369,108 - GFF2942 0.51 -0.2 369,275 + GFF2942 0.69 +0.4 369,275 + GFF2942 0.69 -0.4 369,275 + GFF2942 0.69 +0.9 369,275 + GFF2942 0.69 +1.1 369,667 + GFF2943 0.13 -0.6 369,668 - GFF2943 0.13 -0.3 369,712 + GFF2943 0.18 +0.5 369,712 + GFF2943 0.18 -0.0 369,712 + GFF2943 0.18 -0.5 369,713 - GFF2943 0.18 -0.3 369,889 + GFF2943 0.38 -0.6 369,889 + GFF2943 0.38 -0.5 369,889 + GFF2943 0.38 -0.5 369,889 + GFF2943 0.38 +0.2 369,889 + GFF2943 0.38 +0.5 369,889 + GFF2943 0.38 -1.3 369,890 - GFF2943 0.38 +0.0 369,890 - GFF2943 0.38 -0.7 369,890 - GFF2943 0.38 -0.7 369,890 - GFF2943 0.38 -1.0 370,144 + GFF2943 0.67 -0.8 370,145 - GFF2943 0.67 -0.5 370,357 + -1.0 370,357 + -0.6 370,358 - +0.0 370,369 + +0.1 370,369 + -0.5 370,369 + -0.7 370,369 + -0.4 370,370 - +0.8 370,372 + -0.8 370,372 + -1.6 370,372 + +0.4 370,372 + -1.3 370,372 + -1.3 370,372 + +0.4 370,372 + -0.2 370,372 + +0.9 370,372 + -1.2 370,372 + +0.2 370,372 + +0.1 370,373 - -0.5 370,373 - +0.1 370,373 - +0.1 370,373 - -0.1 370,373 - -1.1 370,373 - -0.5 370,373 - -0.3 370,373 - +1.7 370,373 - +0.7 370,373 - -0.5 370,373 - +0.3 370,373 - +0.3 370,470 + -0.3 370,471 - -0.4 370,729 + GFF2944 0.51 +0.1 370,729 + GFF2944 0.51 +0.4 370,729 + GFF2944 0.51 +1.2 370,729 + GFF2944 0.51 -0.3 370,730 - GFF2944 0.51 +1.3 370,730 - GFF2944 0.51 -0.4 370,730 - GFF2944 0.51 +0.2 370,867 + GFF2944 0.78 -0.8 370,868 - GFF2944 0.78 -0.9 370,868 - GFF2944 0.78 +0.6 370,868 - GFF2944 0.78 -0.5 370,868 - GFF2944 0.78 -0.0 370,868 - GFF2944 0.78 -0.2 370,936 + +0.5 370,936 + +1.4 370,937 - -0.1 371,058 + GFF2945 0.15 +0.7 371,058 + GFF2945 0.15 +0.5 371,058 + GFF2945 0.15 -0.8 371,058 + GFF2945 0.15 +0.0 371,059 - GFF2945 0.15 -0.3 371,059 - GFF2945 0.15 +0.2 371,059 - GFF2945 0.15 +0.3 371,059 - GFF2945 0.15 -0.6 371,059 - GFF2945 0.15 +0.2 371,059 - GFF2945 0.15 -1.1 371,059 - GFF2945 0.15 -0.4 371,059 - GFF2945 0.15 +1.1 371,059 - GFF2945 0.15 -0.1 371,059 - GFF2945 0.15 +0.4 371,059 - GFF2945 0.15 -0.8 371,059 - GFF2945 0.15 -0.2 371,059 - GFF2945 0.15 -0.0 371,059 - GFF2945 0.15 -0.3 371,059 - GFF2945 0.15 -0.9 371,059 - GFF2945 0.15 -0.8 371,059 - GFF2945 0.15 -0.4 371,059 - GFF2945 0.15 -0.9 371,059 - GFF2945 0.15 -0.6 371,067 + GFF2945 0.17 -1.1 371,067 + GFF2945 0.17 -0.3 371,067 + GFF2945 0.17 -0.7 371,068 - GFF2945 0.17 -0.4 371,094 + GFF2945 0.24 +0.6 371,094 + GFF2945 0.24 -0.1 371,094 + GFF2945 0.24 -1.1 371,095 - GFF2945 0.24 -1.0 371,095 - GFF2945 0.24 -0.1 371,095 - GFF2945 0.24 -0.0 371,153 + GFF2945 0.39 -0.6 371,334 + GFF2945 0.85 -0.9 371,334 + GFF2945 0.85 -0.5 371,337 + GFF2945 0.86 +0.4 371,337 + GFF2945 0.86 +1.1 371,337 + GFF2945 0.86 -0.3 371,338 - GFF2945 0.86 -0.3 371,338 - GFF2945 0.86 +0.0 371,338 - GFF2945 0.86 -0.4
Or see this region's nucleotide sequence