Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2941 and GFF2942 are separated by 21 nucleotides GFF2942 and GFF2943 overlap by 4 nucleotides GFF2943 and GFF2944 are separated by 39 nucleotides
GFF2941 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 367,511 to 368,617
GFF2941
GFF2942 - Epoxide hydrolase (EC 3.3.2.9), at 368,639 to 369,559
GFF2942
GFF2943 - S-formylglutathione hydrolase (EC 3.1.2.12), at 369,556 to 370,434
GFF2943
GFF2944 - Acetyltransferase, at 370,474 to 370,977
GFF2944
Position (kb)
368
369
370 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.640 kb on + strand, within GFF2941 at 367.641 kb on - strand, within GFF2941 at 367.641 kb on - strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.643 kb on + strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.644 kb on - strand, within GFF2941 at 367.772 kb on + strand, within GFF2941 at 367.772 kb on + strand, within GFF2941 at 367.773 kb on - strand, within GFF2941 at 367.773 kb on - strand, within GFF2941 at 367.773 kb on - strand, within GFF2941 at 367.773 kb on - strand, within GFF2941 at 367.773 kb on - strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.913 kb on + strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.914 kb on - strand, within GFF2941 at 367.940 kb on + strand, within GFF2941 at 367.940 kb on + strand, within GFF2941 at 367.940 kb on + strand, within GFF2941 at 367.940 kb on + strand, within GFF2941 at 367.940 kb on + strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 367.941 kb on - strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.006 kb on + strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.007 kb on - strand, within GFF2941 at 368.282 kb on + strand, within GFF2941 at 368.282 kb on + strand, within GFF2941 at 368.283 kb on - strand, within GFF2941 at 368.283 kb on - strand, within GFF2941 at 368.283 kb on - strand, within GFF2941 at 368.495 kb on + strand, within GFF2941 at 368.495 kb on + strand, within GFF2941 at 368.495 kb on + strand, within GFF2941 at 368.495 kb on + strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.496 kb on - strand, within GFF2941 at 368.612 kb on + strand at 368.612 kb on + strand at 368.612 kb on + strand at 368.613 kb on - strand at 368.613 kb on - strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.693 kb on + strand at 368.694 kb on - strand at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.765 kb on + strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.766 kb on - strand, within GFF2942 at 368.819 kb on + strand, within GFF2942 at 368.819 kb on + strand, within GFF2942 at 368.820 kb on - strand, within GFF2942 at 368.820 kb on - strand, within GFF2942 at 368.831 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.032 kb on + strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.033 kb on - strand, within GFF2942 at 369.098 kb on + strand, within GFF2942 at 369.099 kb on - strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.104 kb on + strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.105 kb on - strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.107 kb on + strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.108 kb on - strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.275 kb on + strand, within GFF2942 at 369.667 kb on + strand, within GFF2943 at 369.668 kb on - strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.712 kb on + strand, within GFF2943 at 369.713 kb on - strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.889 kb on + strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 369.890 kb on - strand, within GFF2943 at 370.144 kb on + strand, within GFF2943 at 370.145 kb on - strand, within GFF2943 at 370.357 kb on + strand at 370.357 kb on + strand at 370.358 kb on - strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.369 kb on + strand at 370.370 kb on - strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.372 kb on + strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.373 kb on - strand at 370.470 kb on + strand at 370.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 367,640 + GFF2941 0.12 -0.3 367,640 + GFF2941 0.12 +0.6 367,640 + GFF2941 0.12 +0.1 367,640 + GFF2941 0.12 -0.3 367,640 + GFF2941 0.12 -0.4 367,640 + GFF2941 0.12 +0.1 367,640 + GFF2941 0.12 +0.1 367,641 - GFF2941 0.12 -1.0 367,641 - GFF2941 0.12 +0.3 367,643 + GFF2941 0.12 +0.2 367,643 + GFF2941 0.12 -0.8 367,643 + GFF2941 0.12 +0.2 367,643 + GFF2941 0.12 -1.0 367,643 + GFF2941 0.12 +1.7 367,643 + GFF2941 0.12 -0.7 367,643 + GFF2941 0.12 -0.4 367,643 + GFF2941 0.12 +0.4 367,643 + GFF2941 0.12 +0.6 367,643 + GFF2941 0.12 +1.3 367,643 + GFF2941 0.12 -0.6 367,643 + GFF2941 0.12 +1.0 367,644 - GFF2941 0.12 -0.5 367,644 - GFF2941 0.12 -1.1 367,644 - GFF2941 0.12 +0.0 367,644 - GFF2941 0.12 +0.0 367,644 - GFF2941 0.12 -0.4 367,644 - GFF2941 0.12 +0.4 367,644 - GFF2941 0.12 +0.2 367,644 - GFF2941 0.12 -0.4 367,644 - GFF2941 0.12 -0.4 367,644 - GFF2941 0.12 -0.5 367,772 + GFF2941 0.24 -1.8 367,772 + GFF2941 0.24 +0.1 367,773 - GFF2941 0.24 -0.6 367,773 - GFF2941 0.24 +0.6 367,773 - GFF2941 0.24 +0.3 367,773 - GFF2941 0.24 +0.1 367,773 - GFF2941 0.24 -0.3 367,913 + GFF2941 0.36 +0.1 367,913 + GFF2941 0.36 -0.1 367,913 + GFF2941 0.36 -0.4 367,913 + GFF2941 0.36 +0.7 367,913 + GFF2941 0.36 -1.2 367,913 + GFF2941 0.36 +0.1 367,913 + GFF2941 0.36 +1.2 367,913 + GFF2941 0.36 -1.0 367,913 + GFF2941 0.36 -0.1 367,913 + GFF2941 0.36 +1.0 367,914 - GFF2941 0.36 +1.0 367,914 - GFF2941 0.36 -0.0 367,914 - GFF2941 0.36 +1.3 367,914 - GFF2941 0.36 +0.7 367,914 - GFF2941 0.36 -0.1 367,914 - GFF2941 0.36 -0.1 367,914 - GFF2941 0.36 -0.2 367,914 - GFF2941 0.36 -1.3 367,914 - GFF2941 0.36 +0.1 367,914 - GFF2941 0.36 +0.4 367,914 - GFF2941 0.36 -0.6 367,914 - GFF2941 0.36 +0.2 367,914 - GFF2941 0.36 +1.7 367,914 - GFF2941 0.36 -1.1 367,914 - GFF2941 0.36 -0.1 367,940 + GFF2941 0.39 +1.0 367,940 + GFF2941 0.39 -0.7 367,940 + GFF2941 0.39 -0.9 367,940 + GFF2941 0.39 -0.3 367,940 + GFF2941 0.39 +0.4 367,941 - GFF2941 0.39 -1.5 367,941 - GFF2941 0.39 -0.2 367,941 - GFF2941 0.39 +0.4 367,941 - GFF2941 0.39 -0.8 367,941 - GFF2941 0.39 +0.3 367,941 - GFF2941 0.39 -0.2 367,941 - GFF2941 0.39 -1.6 368,006 + GFF2941 0.45 -0.2 368,006 + GFF2941 0.45 +0.8 368,006 + GFF2941 0.45 -0.3 368,006 + GFF2941 0.45 +1.2 368,006 + GFF2941 0.45 -1.0 368,006 + GFF2941 0.45 +0.2 368,007 - GFF2941 0.45 -0.5 368,007 - GFF2941 0.45 +0.4 368,007 - GFF2941 0.45 -0.3 368,007 - GFF2941 0.45 -1.9 368,007 - GFF2941 0.45 -0.2 368,007 - GFF2941 0.45 +0.1 368,007 - GFF2941 0.45 +0.4 368,282 + GFF2941 0.70 -1.2 368,282 + GFF2941 0.70 +0.6 368,283 - GFF2941 0.70 +0.1 368,283 - GFF2941 0.70 +0.1 368,283 - GFF2941 0.70 -0.7 368,495 + GFF2941 0.89 -0.5 368,495 + GFF2941 0.89 -0.5 368,495 + GFF2941 0.89 -1.0 368,495 + GFF2941 0.89 -0.0 368,496 - GFF2941 0.89 -0.9 368,496 - GFF2941 0.89 -1.1 368,496 - GFF2941 0.89 -0.1 368,496 - GFF2941 0.89 -1.1 368,496 - GFF2941 0.89 +0.6 368,496 - GFF2941 0.89 +0.4 368,496 - GFF2941 0.89 +0.7 368,496 - GFF2941 0.89 -2.1 368,496 - GFF2941 0.89 +0.3 368,496 - GFF2941 0.89 -0.6 368,612 + +1.7 368,612 + +0.2 368,612 + +0.9 368,613 - +0.3 368,613 - -0.1 368,693 + -0.3 368,693 + -1.5 368,693 + -0.8 368,693 + -0.5 368,694 - +0.2 368,765 + GFF2942 0.14 -0.3 368,765 + GFF2942 0.14 -0.3 368,765 + GFF2942 0.14 -0.7 368,765 + GFF2942 0.14 +0.4 368,765 + GFF2942 0.14 -2.5 368,765 + GFF2942 0.14 +1.7 368,765 + GFF2942 0.14 +1.5 368,765 + GFF2942 0.14 -0.4 368,765 + GFF2942 0.14 -0.9 368,765 + GFF2942 0.14 -0.8 368,765 + GFF2942 0.14 -1.5 368,765 + GFF2942 0.14 -1.5 368,766 - GFF2942 0.14 -0.3 368,766 - GFF2942 0.14 +0.6 368,766 - GFF2942 0.14 -1.5 368,766 - GFF2942 0.14 +2.3 368,766 - GFF2942 0.14 +0.4 368,819 + GFF2942 0.20 -0.8 368,819 + GFF2942 0.20 -0.6 368,820 - GFF2942 0.20 -0.9 368,820 - GFF2942 0.20 +0.3 368,831 + GFF2942 0.21 +0.6 369,032 + GFF2942 0.43 -1.5 369,032 + GFF2942 0.43 +0.4 369,032 + GFF2942 0.43 -0.5 369,032 + GFF2942 0.43 +0.7 369,033 - GFF2942 0.43 +0.0 369,033 - GFF2942 0.43 +0.0 369,033 - GFF2942 0.43 -0.1 369,033 - GFF2942 0.43 -0.6 369,033 - GFF2942 0.43 -0.2 369,033 - GFF2942 0.43 -1.3 369,033 - GFF2942 0.43 -0.5 369,098 + GFF2942 0.50 +0.5 369,099 - GFF2942 0.50 +0.2 369,104 + GFF2942 0.50 -1.1 369,104 + GFF2942 0.50 -1.1 369,104 + GFF2942 0.50 +0.4 369,104 + GFF2942 0.50 -0.3 369,104 + GFF2942 0.50 -0.4 369,104 + GFF2942 0.50 -0.1 369,104 + GFF2942 0.50 +0.3 369,104 + GFF2942 0.50 -1.7 369,105 - GFF2942 0.51 +0.4 369,105 - GFF2942 0.51 +0.0 369,105 - GFF2942 0.51 -1.1 369,105 - GFF2942 0.51 -2.0 369,105 - GFF2942 0.51 +0.5 369,105 - GFF2942 0.51 +1.1 369,105 - GFF2942 0.51 +1.0 369,105 - GFF2942 0.51 +1.4 369,105 - GFF2942 0.51 -0.3 369,105 - GFF2942 0.51 +0.3 369,105 - GFF2942 0.51 +0.1 369,105 - GFF2942 0.51 +0.2 369,107 + GFF2942 0.51 -0.1 369,107 + GFF2942 0.51 +0.8 369,107 + GFF2942 0.51 +0.2 369,107 + GFF2942 0.51 -1.2 369,107 + GFF2942 0.51 -0.3 369,107 + GFF2942 0.51 -0.2 369,107 + GFF2942 0.51 +0.7 369,108 - GFF2942 0.51 +0.3 369,108 - GFF2942 0.51 +0.1 369,108 - GFF2942 0.51 +0.1 369,108 - GFF2942 0.51 -0.1 369,108 - GFF2942 0.51 -0.5 369,108 - GFF2942 0.51 +1.0 369,108 - GFF2942 0.51 -0.2 369,275 + GFF2942 0.69 +0.4 369,275 + GFF2942 0.69 -0.4 369,275 + GFF2942 0.69 +0.9 369,275 + GFF2942 0.69 +1.1 369,667 + GFF2943 0.13 -0.6 369,668 - GFF2943 0.13 -0.3 369,712 + GFF2943 0.18 +0.5 369,712 + GFF2943 0.18 -0.0 369,712 + GFF2943 0.18 -0.5 369,713 - GFF2943 0.18 -0.3 369,889 + GFF2943 0.38 -0.6 369,889 + GFF2943 0.38 -0.5 369,889 + GFF2943 0.38 -0.5 369,889 + GFF2943 0.38 +0.2 369,889 + GFF2943 0.38 +0.5 369,889 + GFF2943 0.38 -1.3 369,890 - GFF2943 0.38 +0.0 369,890 - GFF2943 0.38 -0.7 369,890 - GFF2943 0.38 -0.7 369,890 - GFF2943 0.38 -1.0 370,144 + GFF2943 0.67 -0.8 370,145 - GFF2943 0.67 -0.5 370,357 + -1.0 370,357 + -0.6 370,358 - +0.0 370,369 + +0.1 370,369 + -0.5 370,369 + -0.7 370,369 + -0.4 370,370 - +0.8 370,372 + -0.8 370,372 + -1.6 370,372 + +0.4 370,372 + -1.3 370,372 + -1.3 370,372 + +0.4 370,372 + -0.2 370,372 + +0.9 370,372 + -1.2 370,372 + +0.2 370,372 + +0.1 370,373 - -0.5 370,373 - +0.1 370,373 - +0.1 370,373 - -0.1 370,373 - -1.1 370,373 - -0.5 370,373 - -0.3 370,373 - +1.7 370,373 - +0.7 370,373 - -0.5 370,373 - +0.3 370,373 - +0.3 370,470 + -0.3 370,471 - -0.4
Or see this region's nucleotide sequence