Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2911 and GFF2912 are separated by 99 nucleotides GFF2912 and GFF2913 overlap by 4 nucleotides GFF2913 and GFF2914 overlap by 17 nucleotides GFF2914 and GFF2915 are separated by 56 nucleotides
GFF2911 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 339,009 to 339,893
GFF2911
GFF2912 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 339,993 to 340,742
GFF2912
GFF2913 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 340,739 to 341,395
GFF2913
GFF2914 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 341,379 to 342,107
GFF2914
GFF2915 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 342,164 to 342,958
GFF2915
Position (kb)
340
341
342 Strain fitness (log2 ratio)
-2
-1
0
1 at 339.872 kb on + strand at 339.905 kb on + strand at 339.906 kb on - strand at 339.918 kb on - strand at 339.999 kb on + strand at 339.999 kb on + strand at 339.999 kb on + strand at 339.999 kb on + strand at 340.000 kb on - strand at 340.000 kb on - strand at 340.000 kb on - strand at 340.000 kb on - strand at 340.000 kb on - strand at 340.000 kb on - strand at 340.029 kb on + strand at 340.029 kb on + strand at 340.029 kb on + strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.030 kb on - strand at 340.170 kb on + strand, within GFF2912 at 340.170 kb on + strand, within GFF2912 at 340.170 kb on + strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.171 kb on - strand, within GFF2912 at 340.222 kb on - strand, within GFF2912 at 340.222 kb on - strand, within GFF2912 at 340.222 kb on - strand, within GFF2912 at 340.302 kb on + strand, within GFF2912 at 340.434 kb on + strand, within GFF2912 at 340.434 kb on + strand, within GFF2912 at 340.435 kb on - strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.584 kb on + strand, within GFF2912 at 340.585 kb on - strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.775 kb on + strand at 340.775 kb on + strand at 340.775 kb on + strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.907 kb on + strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.958 kb on + strand, within GFF2913 at 340.959 kb on - strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.321 kb on + strand, within GFF2913 at 341.322 kb on - strand, within GFF2913 at 341.410 kb on - strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.643 kb on + strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.010 kb on - strand, within GFF2914 at 342.105 kb on + strand at 342.106 kb on - strand at 342.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 339,872 + -1.3 339,905 + -0.1 339,906 - -0.2 339,918 - +0.5 339,999 + -0.6 339,999 + -1.1 339,999 + -0.0 339,999 + -0.1 340,000 - -0.5 340,000 - -0.1 340,000 - -0.8 340,000 - +0.7 340,000 - +0.4 340,000 - -0.2 340,029 + +1.4 340,029 + +1.2 340,029 + -0.7 340,030 - +1.1 340,030 - -1.3 340,030 - +0.1 340,030 - -1.3 340,030 - +0.3 340,030 - -0.8 340,030 - +0.4 340,170 + GFF2912 0.24 -0.6 340,170 + GFF2912 0.24 -0.3 340,170 + GFF2912 0.24 -0.3 340,171 - GFF2912 0.24 +0.2 340,171 - GFF2912 0.24 +0.1 340,171 - GFF2912 0.24 -0.2 340,171 - GFF2912 0.24 -0.3 340,171 - GFF2912 0.24 +0.0 340,171 - GFF2912 0.24 +1.2 340,171 - GFF2912 0.24 -0.0 340,222 - GFF2912 0.31 -0.6 340,222 - GFF2912 0.31 +1.0 340,222 - GFF2912 0.31 -0.3 340,302 + GFF2912 0.41 -0.8 340,434 + GFF2912 0.59 +0.1 340,434 + GFF2912 0.59 -0.1 340,435 - GFF2912 0.59 +1.1 340,563 + GFF2912 0.76 -0.9 340,563 + GFF2912 0.76 -0.6 340,563 + GFF2912 0.76 -0.5 340,563 + GFF2912 0.76 -0.6 340,563 + GFF2912 0.76 -0.3 340,563 + GFF2912 0.76 -0.6 340,564 - GFF2912 0.76 -0.4 340,564 - GFF2912 0.76 -1.1 340,584 + GFF2912 0.79 -0.0 340,585 - GFF2912 0.79 -0.8 340,635 + GFF2912 0.86 +0.2 340,635 + GFF2912 0.86 -0.5 340,635 + GFF2912 0.86 +0.0 340,635 + GFF2912 0.86 -1.1 340,635 + GFF2912 0.86 +1.4 340,775 + -0.1 340,775 + -0.4 340,775 + -1.9 340,776 - +1.5 340,776 - -2.2 340,776 - +0.1 340,776 - +0.4 340,776 - +0.1 340,907 + GFF2913 0.26 +1.2 340,908 - GFF2913 0.26 -2.1 340,908 - GFF2913 0.26 -0.9 340,908 - GFF2913 0.26 +0.8 340,908 - GFF2913 0.26 -0.5 340,958 + GFF2913 0.33 -1.3 340,959 - GFF2913 0.33 +0.5 341,027 + GFF2913 0.44 -0.9 341,027 + GFF2913 0.44 -1.6 341,028 - GFF2913 0.44 +0.6 341,028 - GFF2913 0.44 +0.1 341,028 - GFF2913 0.44 -1.2 341,321 + GFF2913 0.89 -1.0 341,322 - GFF2913 0.89 -0.6 341,410 - +1.4 341,411 + +0.7 341,411 + +0.5 341,411 + +0.7 341,412 - +0.7 341,412 - -0.4 341,412 - -0.7 341,643 + GFF2914 0.36 -1.3 341,644 - GFF2914 0.36 -0.3 341,644 - GFF2914 0.36 -1.3 341,644 - GFF2914 0.36 -0.2 341,644 - GFF2914 0.36 +0.7 341,644 - GFF2914 0.36 -0.3 341,644 - GFF2914 0.36 -0.1 342,009 + GFF2914 0.86 +1.4 342,009 + GFF2914 0.86 -1.3 342,009 + GFF2914 0.86 +1.0 342,009 + GFF2914 0.86 +1.4 342,009 + GFF2914 0.86 -2.5 342,010 - GFF2914 0.87 +1.7 342,105 + +0.0 342,106 - -0.2 342,141 - -1.1
Or see this region's nucleotide sequence