Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2886 and GFF2887 overlap by 4 nucleotides GFF2887 and GFF2888 are separated by 7 nucleotides GFF2888 and GFF2889 overlap by 8 nucleotides GFF2889 and GFF2890 overlap by 4 nucleotides
GFF2886 - Metallo-beta-lactamase superfamily protein PA0057, at 312,398 to 313,297
GFF2886
GFF2887 - Thioredoxin-like protein clustered with PA0057, at 313,294 to 314,025
GFF2887
GFF2888 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 314,033 to 314,737
GFF2888
GFF2889 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 314,730 to 315,491
GFF2889
GFF2890 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 315,488 to 316,486
GFF2890
Position (kb) 314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 313.082 kb on + strand, within GFF2886 at 313.082 kb on + strand, within GFF2886 at 313.083 kb on - strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.146 kb on - strand, within GFF2886 at 313.146 kb on - strand, within GFF2886 at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.480 kb on + strand, within GFF2887 at 313.481 kb on - strand, within GFF2887 at 313.481 kb on - strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.673 kb on - strand, within GFF2887 at 313.799 kb on + strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.998 kb on - strand at 313.998 kb on - strand at 314.394 kb on + strand, within GFF2888 at 314.394 kb on + strand, within GFF2888 at 314.394 kb on + strand, within GFF2888 at 314.395 kb on - strand, within GFF2888 at 314.395 kb on - strand, within GFF2888 at 314.718 kb on - strand at 314.718 kb on - strand at 314.761 kb on + strand at 314.761 kb on + strand at 314.761 kb on + strand at 314.761 kb on + strand at 314.761 kb on + strand at 314.761 kb on + strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 314.762 kb on - strand at 315.041 kb on - strand, within GFF2889 at 315.603 kb on + strand, within GFF2890 at 315.603 kb on + strand, within GFF2890 at 315.604 kb on - strand, within GFF2890 at 315.673 kb on - strand, within GFF2890
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 313,082 + GFF2886 0.76 -2.9 313,082 + GFF2886 0.76 -0.1 313,083 - GFF2886 0.76 +0.1 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -1.4 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -0.4 313,146 - GFF2886 0.83 -0.2 313,146 - GFF2886 0.83 +0.1 313,330 + -1.2 313,330 + -0.2 313,330 + -0.7 313,330 + -0.1 313,330 + -0.7 313,330 + -1.2 313,330 + -1.9 313,330 + +0.2 313,330 + +0.5 313,330 + +1.3 313,330 + -0.3 313,330 + -0.5 313,330 + -0.2 313,331 - -0.6 313,331 - -0.6 313,331 - +0.5 313,331 - -0.9 313,331 - -0.4 313,331 - -0.9 313,331 - -0.7 313,331 - +0.8 313,331 - -1.3 313,331 - -0.2 313,331 - -0.3 313,331 - -0.2 313,331 - -1.1 313,331 - -0.4 313,331 - +2.2 313,331 - -0.8 313,331 - +0.3 313,360 + -1.5 313,360 + -1.2 313,360 + -0.5 313,360 + +0.2 313,360 + -0.5 313,361 - -1.6 313,361 - -0.1 313,361 - +0.8 313,361 - -0.1 313,361 - -0.2 313,480 + GFF2887 0.25 -0.4 313,481 - GFF2887 0.26 -0.3 313,481 - GFF2887 0.26 -1.6 313,540 + GFF2887 0.34 -0.7 313,540 + GFF2887 0.34 +0.5 313,540 + GFF2887 0.34 +0.7 313,541 - GFF2887 0.34 +0.7 313,541 - GFF2887 0.34 -0.5 313,541 - GFF2887 0.34 +1.7 313,541 - GFF2887 0.34 -0.2 313,541 - GFF2887 0.34 +0.0 313,672 + GFF2887 0.52 +0.2 313,672 + GFF2887 0.52 -0.6 313,672 + GFF2887 0.52 -1.3 313,672 + GFF2887 0.52 +0.0 313,673 - GFF2887 0.52 +3.0 313,799 + GFF2887 0.69 -0.3 313,800 - GFF2887 0.69 -0.1 313,800 - GFF2887 0.69 +1.2 313,800 - GFF2887 0.69 -0.5 313,800 - GFF2887 0.69 -0.1 313,998 - -0.7 313,998 - +0.7 314,394 + GFF2888 0.51 +0.7 314,394 + GFF2888 0.51 -2.8 314,394 + GFF2888 0.51 -1.2 314,395 - GFF2888 0.51 -0.1 314,395 - GFF2888 0.51 -0.8 314,718 - -0.9 314,718 - -1.5 314,761 + -0.9 314,761 + +1.2 314,761 + -0.1 314,761 + -0.1 314,761 + +0.4 314,761 + -1.8 314,762 - +0.3 314,762 - -0.2 314,762 - -0.2 314,762 - -2.2 314,762 - +0.9 314,762 - +0.1 314,762 - +0.4 314,762 - -0.2 315,041 - GFF2889 0.41 +0.7 315,603 + GFF2890 0.12 -0.8 315,603 + GFF2890 0.12 +0.0 315,604 - GFF2890 0.12 -0.8 315,673 - GFF2890 0.19 -0.3
Or see this region's nucleotide sequence