Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2885 and GFF2886 are separated by 144 nucleotides GFF2886 and GFF2887 overlap by 4 nucleotides GFF2887 and GFF2888 are separated by 7 nucleotides
GFF2885 - Transcriptional regulator, LysR family, at 311,351 to 312,253
GFF2885
GFF2886 - Metallo-beta-lactamase superfamily protein PA0057, at 312,398 to 313,297
GFF2886
GFF2887 - Thioredoxin-like protein clustered with PA0057, at 313,294 to 314,025
GFF2887
GFF2888 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 314,033 to 314,737
GFF2888
Position (kb)
312
313
314 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 311.612 kb on + strand, within GFF2885 at 311.613 kb on - strand, within GFF2885 at 311.613 kb on - strand, within GFF2885 at 311.722 kb on + strand, within GFF2885 at 311.723 kb on - strand, within GFF2885 at 311.843 kb on + strand, within GFF2885 at 311.843 kb on + strand, within GFF2885 at 311.843 kb on + strand, within GFF2885 at 311.844 kb on - strand, within GFF2885 at 311.844 kb on - strand, within GFF2885 at 312.119 kb on + strand, within GFF2885 at 312.119 kb on + strand, within GFF2885 at 312.337 kb on + strand at 312.337 kb on + strand at 312.337 kb on + strand at 312.497 kb on + strand, within GFF2886 at 312.497 kb on + strand, within GFF2886 at 312.497 kb on + strand, within GFF2886 at 312.497 kb on + strand, within GFF2886 at 312.497 kb on + strand, within GFF2886 at 312.497 kb on + strand, within GFF2886 at 312.498 kb on - strand, within GFF2886 at 312.498 kb on - strand, within GFF2886 at 312.498 kb on - strand, within GFF2886 at 312.498 kb on - strand, within GFF2886 at 312.498 kb on - strand, within GFF2886 at 312.650 kb on + strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.651 kb on - strand, within GFF2886 at 312.674 kb on + strand, within GFF2886 at 312.674 kb on + strand, within GFF2886 at 312.674 kb on + strand, within GFF2886 at 312.675 kb on - strand, within GFF2886 at 312.675 kb on - strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.677 kb on + strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.678 kb on - strand, within GFF2886 at 312.980 kb on - strand, within GFF2886 at 313.082 kb on + strand, within GFF2886 at 313.082 kb on + strand, within GFF2886 at 313.083 kb on - strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.112 kb on + strand, within GFF2886 at 313.146 kb on - strand, within GFF2886 at 313.146 kb on - strand, within GFF2886 at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.330 kb on + strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.331 kb on - strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.360 kb on + strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.361 kb on - strand at 313.480 kb on + strand, within GFF2887 at 313.481 kb on - strand, within GFF2887 at 313.481 kb on - strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.540 kb on + strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.541 kb on - strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.672 kb on + strand, within GFF2887 at 313.673 kb on - strand, within GFF2887 at 313.799 kb on + strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.800 kb on - strand, within GFF2887 at 313.998 kb on - strand at 313.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 311,612 + GFF2885 0.29 -0.7 311,613 - GFF2885 0.29 +1.2 311,613 - GFF2885 0.29 -0.3 311,722 + GFF2885 0.41 -0.4 311,723 - GFF2885 0.41 +0.0 311,843 + GFF2885 0.54 -1.1 311,843 + GFF2885 0.54 -0.6 311,843 + GFF2885 0.54 +1.1 311,844 - GFF2885 0.55 +0.5 311,844 - GFF2885 0.55 -0.0 312,119 + GFF2885 0.85 -2.4 312,119 + GFF2885 0.85 -1.3 312,337 + -2.8 312,337 + -0.3 312,337 + -0.9 312,497 + GFF2886 0.11 -1.2 312,497 + GFF2886 0.11 -0.6 312,497 + GFF2886 0.11 -0.4 312,497 + GFF2886 0.11 +0.1 312,497 + GFF2886 0.11 +1.1 312,497 + GFF2886 0.11 -0.1 312,498 - GFF2886 0.11 -0.8 312,498 - GFF2886 0.11 -0.2 312,498 - GFF2886 0.11 -0.6 312,498 - GFF2886 0.11 -0.3 312,498 - GFF2886 0.11 -1.2 312,650 + GFF2886 0.28 -0.7 312,651 - GFF2886 0.28 -0.5 312,651 - GFF2886 0.28 +0.2 312,651 - GFF2886 0.28 +0.0 312,651 - GFF2886 0.28 -0.7 312,651 - GFF2886 0.28 -1.4 312,651 - GFF2886 0.28 -1.6 312,651 - GFF2886 0.28 -0.9 312,674 + GFF2886 0.31 +0.3 312,674 + GFF2886 0.31 -1.3 312,674 + GFF2886 0.31 -0.4 312,675 - GFF2886 0.31 -0.2 312,675 - GFF2886 0.31 +1.3 312,677 + GFF2886 0.31 -0.3 312,677 + GFF2886 0.31 -0.1 312,677 + GFF2886 0.31 -0.2 312,677 + GFF2886 0.31 -1.1 312,677 + GFF2886 0.31 -2.4 312,677 + GFF2886 0.31 +1.2 312,678 - GFF2886 0.31 +0.5 312,678 - GFF2886 0.31 -0.4 312,678 - GFF2886 0.31 +0.5 312,678 - GFF2886 0.31 -0.4 312,678 - GFF2886 0.31 +0.1 312,678 - GFF2886 0.31 +0.5 312,980 - GFF2886 0.65 -1.2 313,082 + GFF2886 0.76 -2.9 313,082 + GFF2886 0.76 -0.1 313,083 - GFF2886 0.76 +0.1 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -1.4 313,112 + GFF2886 0.79 -0.1 313,112 + GFF2886 0.79 -0.4 313,146 - GFF2886 0.83 -0.2 313,146 - GFF2886 0.83 +0.1 313,330 + -1.2 313,330 + -0.2 313,330 + -0.7 313,330 + -0.1 313,330 + -0.7 313,330 + -1.2 313,330 + -1.9 313,330 + +0.2 313,330 + +0.5 313,330 + +1.3 313,330 + -0.3 313,330 + -0.5 313,330 + -0.2 313,331 - -0.6 313,331 - -0.6 313,331 - +0.5 313,331 - -0.9 313,331 - -0.4 313,331 - -0.9 313,331 - -0.7 313,331 - +0.8 313,331 - -1.3 313,331 - -0.2 313,331 - -0.3 313,331 - -0.2 313,331 - -1.1 313,331 - -0.4 313,331 - +2.2 313,331 - -0.8 313,331 - +0.3 313,360 + -1.5 313,360 + -1.2 313,360 + -0.5 313,360 + +0.2 313,360 + -0.5 313,361 - -1.6 313,361 - -0.1 313,361 - +0.8 313,361 - -0.1 313,361 - -0.2 313,480 + GFF2887 0.25 -0.4 313,481 - GFF2887 0.26 -0.3 313,481 - GFF2887 0.26 -1.6 313,540 + GFF2887 0.34 -0.7 313,540 + GFF2887 0.34 +0.5 313,540 + GFF2887 0.34 +0.7 313,541 - GFF2887 0.34 +0.7 313,541 - GFF2887 0.34 -0.5 313,541 - GFF2887 0.34 +1.7 313,541 - GFF2887 0.34 -0.2 313,541 - GFF2887 0.34 +0.0 313,672 + GFF2887 0.52 +0.2 313,672 + GFF2887 0.52 -0.6 313,672 + GFF2887 0.52 -1.3 313,672 + GFF2887 0.52 +0.0 313,673 - GFF2887 0.52 +3.0 313,799 + GFF2887 0.69 -0.3 313,800 - GFF2887 0.69 -0.1 313,800 - GFF2887 0.69 +1.2 313,800 - GFF2887 0.69 -0.5 313,800 - GFF2887 0.69 -0.1 313,998 - -0.7 313,998 - +0.7
Or see this region's nucleotide sequence