Strain Fitness in Variovorax sp. SCN45 around GFF2206

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2205 and GFF2206 overlap by 4 nucleotidesGFF2206 and GFF2207 overlap by 4 nucleotidesGFF2207 and GFF2208 overlap by 8 nucleotides GFF2205 - hypothetical protein, at 266,597 to 267,580 GFF2205 GFF2206 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 267,577 to 268,539 GFF2206 GFF2207 - Flp pilus assembly protein TadB, at 268,536 to 269,534 GFF2207 GFF2208 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 269,527 to 270,840 GFF2208 Position (kb) 267 268 269Strain fitness (log2 ratio) -1 0 1 2 3at 266.606 kb on - strandat 266.937 kb on + strand, within GFF2205at 267.120 kb on + strand, within GFF2205at 267.196 kb on + strand, within GFF2205at 267.197 kb on - strand, within GFF2205at 267.197 kb on - strand, within GFF2205at 267.198 kb on + strand, within GFF2205at 267.198 kb on + strand, within GFF2205at 267.199 kb on - strand, within GFF2205at 267.199 kb on - strand, within GFF2205at 267.360 kb on + strand, within GFF2205at 267.360 kb on + strand, within GFF2205at 267.360 kb on + strand, within GFF2205at 267.563 kb on + strandat 267.564 kb on - strandat 267.887 kb on + strand, within GFF2206at 267.922 kb on + strand, within GFF2206at 267.923 kb on - strand, within GFF2206at 267.923 kb on - strand, within GFF2206at 268.560 kb on + strandat 268.560 kb on + strandat 268.560 kb on + strandat 268.560 kb on + strandat 268.560 kb on + strandat 268.561 kb on - strandat 268.600 kb on + strandat 268.600 kb on + strandat 268.601 kb on - strandat 268.601 kb on - strandat 268.601 kb on - strandat 268.604 kb on + strandat 268.604 kb on + strandat 268.605 kb on - strandat 268.659 kb on - strand, within GFF2207at 269.037 kb on + strand, within GFF2207at 269.041 kb on + strand, within GFF2207at 269.042 kb on - strand, within GFF2207at 269.172 kb on + strand, within GFF2207at 269.173 kb on - strand, within GFF2207at 269.173 kb on - strand, within GFF2207at 269.173 kb on - strand, within GFF2207at 269.300 kb on + strand, within GFF2207

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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266,606 - -1.4
266,937 + GFF2205 0.35 +0.5
267,120 + GFF2205 0.53 -1.0
267,196 + GFF2205 0.61 -0.7
267,197 - GFF2205 0.61 -0.5
267,197 - GFF2205 0.61 -1.2
267,198 + GFF2205 0.61 +0.3
267,198 + GFF2205 0.61 +0.8
267,199 - GFF2205 0.61 +0.8
267,199 - GFF2205 0.61 -0.1
267,360 + GFF2205 0.78 -0.5
267,360 + GFF2205 0.78 +0.4
267,360 + GFF2205 0.78 -0.9
267,563 + +0.9
267,564 - -1.7
267,887 + GFF2206 0.32 -0.2
267,922 + GFF2206 0.36 +0.4
267,923 - GFF2206 0.36 +0.5
267,923 - GFF2206 0.36 +3.5
268,560 + +0.1
268,560 + +0.9
268,560 + +0.8
268,560 + +1.1
268,560 + -0.6
268,561 - -1.0
268,600 + +0.3
268,600 + +2.2
268,601 - -0.1
268,601 - -1.3
268,601 - -1.5
268,604 + -0.5
268,604 + +0.2
268,605 - -0.6
268,659 - GFF2207 0.12 -0.8
269,037 + GFF2207 0.50 -0.6
269,041 + GFF2207 0.51 +0.9
269,042 - GFF2207 0.51 +0.7
269,172 + GFF2207 0.64 -0.5
269,173 - GFF2207 0.64 -1.6
269,173 - GFF2207 0.64 +0.5
269,173 - GFF2207 0.64 +0.3
269,300 + GFF2207 0.76 -0.5

Or see this region's nucleotide sequence