Strain Fitness in Variovorax sp. SCN45 around GFF195

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF194 and GFF195 are separated by 0 nucleotidesGFF195 and GFF196 are separated by 105 nucleotidesGFF196 and GFF197 are separated by 26 nucleotides GFF194 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 223,605 to 224,699 GFF194 GFF195 - RidA/YER057c/UK114 superfamily, group 1, at 224,700 to 225,191 GFF195 GFF196 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 225,297 to 225,617 GFF196 GFF197 - BUG/TctC family periplasmic protein, at 225,644 to 226,612 GFF197 Position (kb) 224 225 226Strain fitness (log2 ratio) -2 -1 0 1 2 3at 223.774 kb on + strand, within GFF194at 223.775 kb on - strand, within GFF194at 223.775 kb on - strand, within GFF194at 223.830 kb on + strand, within GFF194at 223.831 kb on - strand, within GFF194at 223.831 kb on - strand, within GFF194at 223.831 kb on - strand, within GFF194at 223.867 kb on - strand, within GFF194at 223.954 kb on - strand, within GFF194at 223.977 kb on + strand, within GFF194at 223.977 kb on + strand, within GFF194at 224.494 kb on - strand, within GFF194at 224.514 kb on + strand, within GFF194at 224.515 kb on - strand, within GFF194at 224.515 kb on - strand, within GFF194at 224.767 kb on + strand, within GFF195at 224.767 kb on + strand, within GFF195at 224.767 kb on + strand, within GFF195at 224.768 kb on - strand, within GFF195at 225.067 kb on + strand, within GFF195at 225.067 kb on + strand, within GFF195at 225.067 kb on + strand, within GFF195at 225.067 kb on + strand, within GFF195at 225.067 kb on + strand, within GFF195at 225.068 kb on - strand, within GFF195at 225.068 kb on - strand, within GFF195at 225.068 kb on - strand, within GFF195at 225.068 kb on - strand, within GFF195at 225.100 kb on + strand, within GFF195at 225.100 kb on + strand, within GFF195at 225.100 kb on + strand, within GFF195at 225.100 kb on + strand, within GFF195at 225.101 kb on - strand, within GFF195at 225.101 kb on - strand, within GFF195at 225.101 kb on - strand, within GFF195at 225.101 kb on - strand, within GFF195at 225.191 kb on + strandat 225.191 kb on + strandat 225.191 kb on + strandat 225.191 kb on + strandat 225.192 kb on - strandat 225.192 kb on - strandat 225.192 kb on - strandat 225.192 kb on - strandat 225.192 kb on - strandat 225.247 kb on + strandat 225.247 kb on + strandat 225.248 kb on - strandat 225.248 kb on - strandat 225.346 kb on + strand, within GFF196at 225.479 kb on + strand, within GFF196at 225.479 kb on + strand, within GFF196at 225.479 kb on + strand, within GFF196at 225.480 kb on - strand, within GFF196at 225.520 kb on + strand, within GFF196at 225.753 kb on + strand, within GFF197at 225.754 kb on - strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.858 kb on + strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 225.859 kb on - strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.068 kb on + strand, within GFF197at 226.069 kb on - strand, within GFF197

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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223,774 + GFF194 0.15 -0.3
223,775 - GFF194 0.16 +0.4
223,775 - GFF194 0.16 -1.4
223,830 + GFF194 0.21 -0.8
223,831 - GFF194 0.21 +0.8
223,831 - GFF194 0.21 +1.4
223,831 - GFF194 0.21 -1.2
223,867 - GFF194 0.24 +0.1
223,954 - GFF194 0.32 +0.2
223,977 + GFF194 0.34 -0.2
223,977 + GFF194 0.34 -0.2
224,494 - GFF194 0.81 +0.4
224,514 + GFF194 0.83 -0.8
224,515 - GFF194 0.83 -0.4
224,515 - GFF194 0.83 -0.2
224,767 + GFF195 0.14 +0.4
224,767 + GFF195 0.14 -0.2
224,767 + GFF195 0.14 +0.4
224,768 - GFF195 0.14 -0.1
225,067 + GFF195 0.75 -0.5
225,067 + GFF195 0.75 +0.1
225,067 + GFF195 0.75 -1.0
225,067 + GFF195 0.75 -1.2
225,067 + GFF195 0.75 +0.2
225,068 - GFF195 0.75 +0.2
225,068 - GFF195 0.75 -0.2
225,068 - GFF195 0.75 +1.4
225,068 - GFF195 0.75 -1.6
225,100 + GFF195 0.81 +0.5
225,100 + GFF195 0.81 -0.2
225,100 + GFF195 0.81 +0.2
225,100 + GFF195 0.81 +0.4
225,101 - GFF195 0.82 +3.2
225,101 - GFF195 0.82 +0.6
225,101 - GFF195 0.82 +0.4
225,101 - GFF195 0.82 +0.4
225,191 + -0.5
225,191 + -0.0
225,191 + -0.7
225,191 + -0.2
225,192 - +2.3
225,192 - -0.0
225,192 - -0.1
225,192 - +1.2
225,192 - -0.6
225,247 + -2.3
225,247 + +0.2
225,248 - -0.5
225,248 - +1.7
225,346 + GFF196 0.15 +0.0
225,479 + GFF196 0.57 +0.5
225,479 + GFF196 0.57 -0.1
225,479 + GFF196 0.57 +0.6
225,480 - GFF196 0.57 -0.3
225,520 + GFF196 0.69 -1.0
225,753 + GFF197 0.11 +0.4
225,754 - GFF197 0.11 +0.2
225,858 + GFF197 0.22 -0.2
225,858 + GFF197 0.22 +0.9
225,858 + GFF197 0.22 -0.6
225,858 + GFF197 0.22 -0.7
225,858 + GFF197 0.22 +1.3
225,859 - GFF197 0.22 +2.6
225,859 - GFF197 0.22 +0.1
225,859 - GFF197 0.22 -0.8
225,859 - GFF197 0.22 -0.7
225,859 - GFF197 0.22 -0.9
225,859 - GFF197 0.22 +0.6
226,068 + GFF197 0.44 -1.2
226,068 + GFF197 0.44 -1.2
226,068 + GFF197 0.44 +1.6
226,068 + GFF197 0.44 -0.4
226,069 - GFF197 0.44 -0.2

Or see this region's nucleotide sequence