Strain Fitness in Variovorax sp. SCN45 around GFF1850

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1848 and GFF1849 are separated by 18 nucleotidesGFF1849 and GFF1850 are separated by 88 nucleotidesGFF1850 and GFF1851 overlap by 8 nucleotides GFF1848 - Proteins incorrectly called adenylate cyclase, at 519,851 to 523,891 GFF1848 GFF1849 - FIG00347667: hypothetical protein, at 523,910 to 524,701 GFF1849 GFF1850 - Tetracenomycin polyketide synthesis O-methyltransferase TcmP, at 524,790 to 525,548 GFF1850 GFF1851 - TOMM biosynthesis cyclodehydratase (protein C) / TOMM biosynthesis docking scaffold (protein D), at 525,541 to 527,778 GFF1851 Position (kb) 524 525 526Strain fitness (log2 ratio) -2 -1 0 1 2 3at 523.961 kb on + strandat 523.962 kb on - strandat 523.962 kb on - strandat 524.096 kb on + strand, within GFF1849at 524.096 kb on + strand, within GFF1849at 524.231 kb on + strand, within GFF1849at 524.231 kb on + strand, within GFF1849at 524.232 kb on - strand, within GFF1849at 524.232 kb on - strand, within GFF1849at 524.232 kb on - strand, within GFF1849at 524.232 kb on - strand, within GFF1849at 524.234 kb on + strand, within GFF1849at 524.234 kb on + strand, within GFF1849at 524.234 kb on + strand, within GFF1849at 524.234 kb on + strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.235 kb on - strand, within GFF1849at 524.483 kb on + strand, within GFF1849at 524.483 kb on + strand, within GFF1849at 524.483 kb on + strand, within GFF1849at 524.484 kb on - strand, within GFF1849at 524.484 kb on - strand, within GFF1849at 524.612 kb on + strand, within GFF1849at 524.663 kb on + strandat 524.663 kb on + strandat 524.663 kb on + strandat 524.663 kb on + strandat 524.663 kb on + strandat 524.663 kb on + strandat 524.663 kb on + strandat 524.664 kb on - strandat 524.664 kb on - strandat 524.664 kb on - strandat 524.664 kb on - strandat 524.664 kb on - strandat 524.684 kb on + strandat 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.868 kb on + strand, within GFF1850at 524.869 kb on - strand, within GFF1850at 524.869 kb on - strand, within GFF1850at 525.210 kb on + strand, within GFF1850at 525.210 kb on + strand, within GFF1850at 525.211 kb on - strand, within GFF1850at 525.211 kb on - strand, within GFF1850at 525.416 kb on + strand, within GFF1850at 525.418 kb on + strand, within GFF1850at 525.418 kb on + strand, within GFF1850at 525.435 kb on + strand, within GFF1850at 525.435 kb on + strand, within GFF1850at 525.435 kb on + strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.436 kb on - strand, within GFF1850at 525.504 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.724 kb on + strandat 525.725 kb on - strandat 525.725 kb on - strandat 525.725 kb on - strandat 525.725 kb on - strandat 525.725 kb on - strandat 525.725 kb on - strandat 525.835 kb on + strand, within GFF1851at 525.835 kb on + strand, within GFF1851at 525.835 kb on + strand, within GFF1851at 525.835 kb on + strand, within GFF1851at 525.835 kb on + strand, within GFF1851at 525.836 kb on - strand, within GFF1851at 525.836 kb on - strand, within GFF1851at 525.836 kb on - strand, within GFF1851at 525.836 kb on - strand, within GFF1851at 525.836 kb on - strand, within GFF1851at 526.027 kb on + strand, within GFF1851at 526.027 kb on + strand, within GFF1851at 526.028 kb on - strand, within GFF1851at 526.028 kb on - strand, within GFF1851at 526.028 kb on - strand, within GFF1851

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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523,961 + -0.4
523,962 - -0.7
523,962 - +1.2
524,096 + GFF1849 0.23 +0.3
524,096 + GFF1849 0.23 -0.4
524,231 + GFF1849 0.41 -0.8
524,231 + GFF1849 0.41 -0.1
524,232 - GFF1849 0.41 -1.2
524,232 - GFF1849 0.41 +0.0
524,232 - GFF1849 0.41 +2.2
524,232 - GFF1849 0.41 +0.4
524,234 + GFF1849 0.41 +0.7
524,234 + GFF1849 0.41 -1.4
524,234 + GFF1849 0.41 -0.0
524,234 + GFF1849 0.41 -0.3
524,235 - GFF1849 0.41 +0.5
524,235 - GFF1849 0.41 +0.5
524,235 - GFF1849 0.41 -0.1
524,235 - GFF1849 0.41 +1.2
524,235 - GFF1849 0.41 +1.1
524,235 - GFF1849 0.41 -0.2
524,483 + GFF1849 0.72 +0.1
524,483 + GFF1849 0.72 +0.3
524,483 + GFF1849 0.72 -0.5
524,484 - GFF1849 0.72 -0.0
524,484 - GFF1849 0.72 -1.5
524,612 + GFF1849 0.89 +0.3
524,663 + -0.6
524,663 + -0.2
524,663 + -0.7
524,663 + +0.8
524,663 + -0.3
524,663 + +0.2
524,663 + -0.0
524,664 - -0.9
524,664 - -0.4
524,664 - +0.6
524,664 - +2.9
524,664 - -0.7
524,684 + -0.3
524,868 + GFF1850 0.10 -0.7
524,868 + GFF1850 0.10 +0.2
524,868 + GFF1850 0.10 +0.6
524,868 + GFF1850 0.10 +2.8
524,868 + GFF1850 0.10 -0.9
524,868 + GFF1850 0.10 -0.6
524,868 + GFF1850 0.10 +0.2
524,868 + GFF1850 0.10 -0.9
524,869 - GFF1850 0.10 -0.5
524,869 - GFF1850 0.10 -1.5
525,210 + GFF1850 0.55 +1.1
525,210 + GFF1850 0.55 +0.2
525,211 - GFF1850 0.55 -2.0
525,211 - GFF1850 0.55 +0.3
525,416 + GFF1850 0.82 -0.4
525,418 + GFF1850 0.83 +0.2
525,418 + GFF1850 0.83 -0.3
525,435 + GFF1850 0.85 -1.2
525,435 + GFF1850 0.85 -0.2
525,435 + GFF1850 0.85 -0.3
525,436 - GFF1850 0.85 -0.8
525,436 - GFF1850 0.85 -0.2
525,436 - GFF1850 0.85 -1.8
525,436 - GFF1850 0.85 +0.0
525,436 - GFF1850 0.85 +0.8
525,436 - GFF1850 0.85 +1.5
525,504 + -0.0
525,724 + +0.5
525,724 + -0.2
525,724 + +0.4
525,724 + -0.3
525,724 + -0.4
525,724 + -0.5
525,724 + -0.6
525,725 - +0.1
525,725 - +0.5
525,725 - -0.3
525,725 - -0.1
525,725 - -1.4
525,725 - -0.8
525,835 + GFF1851 0.13 +0.4
525,835 + GFF1851 0.13 -0.9
525,835 + GFF1851 0.13 +1.2
525,835 + GFF1851 0.13 -0.4
525,835 + GFF1851 0.13 -1.2
525,836 - GFF1851 0.13 +0.1
525,836 - GFF1851 0.13 +1.8
525,836 - GFF1851 0.13 +0.4
525,836 - GFF1851 0.13 -0.2
525,836 - GFF1851 0.13 -0.4
526,027 + GFF1851 0.22 -0.5
526,027 + GFF1851 0.22 +0.2
526,028 - GFF1851 0.22 -0.2
526,028 - GFF1851 0.22 -0.0
526,028 - GFF1851 0.22 -0.2

Or see this region's nucleotide sequence