Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1535 and GFF1536 overlap by 4 nucleotides GFF1536 and GFF1537 are separated by 10 nucleotides
GFF1535 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 133,012 to 133,917
GFF1535
GFF1536 - BUG/TctC family periplasmic protein, at 133,914 to 134,897
GFF1536
GFF1537 - FIG01127817: hypothetical protein, at 134,908 to 136,569
GFF1537
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.972 kb on + strand at 132.998 kb on + strand at 132.998 kb on + strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 133.264 kb on + strand, within GFF1535 at 133.265 kb on - strand, within GFF1535 at 133.265 kb on - strand, within GFF1535 at 133.823 kb on - strand, within GFF1535 at 134.001 kb on + strand at 134.233 kb on - strand, within GFF1536 at 134.248 kb on - strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.386 kb on - strand, within GFF1536 at 134.475 kb on + strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.857 kb on - strand at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.179 kb on - strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.602 kb on - strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.832 kb on + strand, within GFF1537 at 135.833 kb on - strand, within GFF1537 at 135.875 kb on - strand, within GFF1537
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 132,972 + +0.2 132,998 + -0.3 132,998 + -0.4 132,999 - -0.7 132,999 - -1.7 132,999 - +0.1 132,999 - -1.1 132,999 - +0.4 133,264 + GFF1535 0.28 -0.5 133,265 - GFF1535 0.28 -1.4 133,265 - GFF1535 0.28 +0.0 133,823 - GFF1535 0.90 +1.9 134,001 + +0.8 134,233 - GFF1536 0.32 -0.2 134,248 - GFF1536 0.34 +0.2 134,385 + GFF1536 0.48 -0.2 134,385 + GFF1536 0.48 -0.2 134,385 + GFF1536 0.48 +0.1 134,385 + GFF1536 0.48 +2.0 134,386 - GFF1536 0.48 +0.2 134,475 + GFF1536 0.57 +1.8 134,476 - GFF1536 0.57 -2.0 134,476 - GFF1536 0.57 +2.2 134,857 - -0.7 135,145 + GFF1537 0.14 -0.3 135,145 + GFF1537 0.14 -0.0 135,145 + GFF1537 0.14 -0.8 135,146 - GFF1537 0.14 -2.1 135,146 - GFF1537 0.14 -0.2 135,146 - GFF1537 0.14 -1.2 135,175 + GFF1537 0.16 +0.5 135,175 + GFF1537 0.16 +1.2 135,176 - GFF1537 0.16 -0.0 135,176 - GFF1537 0.16 -1.3 135,178 + GFF1537 0.16 -0.1 135,178 + GFF1537 0.16 -0.3 135,179 - GFF1537 0.16 +1.8 135,601 + GFF1537 0.42 +0.2 135,601 + GFF1537 0.42 +0.3 135,602 - GFF1537 0.42 -0.5 135,775 + GFF1537 0.52 +0.1 135,775 + GFF1537 0.52 -0.7 135,775 + GFF1537 0.52 -0.1 135,775 + GFF1537 0.52 -0.8 135,775 + GFF1537 0.52 -0.9 135,776 - GFF1537 0.52 +0.1 135,776 - GFF1537 0.52 -0.1 135,776 - GFF1537 0.52 -1.1 135,832 + GFF1537 0.56 +1.0 135,833 - GFF1537 0.56 -0.2 135,875 - GFF1537 0.58 +0.6
Or see this region's nucleotide sequence