Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF151 and GFF152 are separated by 27 nucleotides GFF152 and GFF153 overlap by 4 nucleotides GFF153 and GFF154 overlap by 4 nucleotides
GFF151 - FIG00460174: hypothetical protein, at 178,819 to 179,706
GFF151
GFF152 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 179,734 to 180,678
GFF152
GFF153 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 180,675 to 181,511
GFF153
GFF154 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 181,508 to 183,178
GFF154
Position (kb) 180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 179.726 kb on + strand at 179.727 kb on - strand at 179.727 kb on - strand at 179.813 kb on + strand at 179.814 kb on - strand at 180.232 kb on + strand, within GFF152 at 180.232 kb on + strand, within GFF152 at 180.233 kb on - strand, within GFF152 at 180.271 kb on + strand, within GFF152 at 180.272 kb on - strand, within GFF152 at 180.272 kb on - strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.643 kb on + strand at 181.116 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 182.273 kb on + strand, within GFF154 at 182.273 kb on + strand, within GFF154 at 182.273 kb on + strand, within GFF154 at 182.373 kb on - strand, within GFF154 at 182.373 kb on - strand, within GFF154 at 182.373 kb on - strand, within GFF154
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 179,726 + -0.6 179,727 - +0.0 179,727 - -1.7 179,813 + -0.2 179,814 - -0.9 180,232 + GFF152 0.53 -0.2 180,232 + GFF152 0.53 -1.9 180,233 - GFF152 0.53 +0.2 180,271 + GFF152 0.57 -1.7 180,272 - GFF152 0.57 +1.7 180,272 - GFF152 0.57 -0.2 180,367 + GFF152 0.67 -0.1 180,367 + GFF152 0.67 +1.0 180,367 + GFF152 0.67 -0.6 180,368 - GFF152 0.67 -0.1 180,368 - GFF152 0.67 -2.4 180,368 - GFF152 0.67 -0.3 180,368 - GFF152 0.67 +1.6 180,578 + GFF152 0.89 -0.3 180,578 + GFF152 0.89 -0.5 180,578 + GFF152 0.89 -0.5 180,579 - GFF152 0.89 +0.1 180,579 - GFF152 0.89 +0.4 180,579 - GFF152 0.89 +0.2 180,579 - GFF152 0.89 -0.4 180,610 + +1.0 180,610 + +1.4 180,610 + -0.6 180,610 + -0.8 180,610 + +0.6 180,611 - +0.1 180,611 - +0.3 180,611 - -0.7 180,611 - +1.8 180,643 + -0.3 181,116 + GFF153 0.53 -1.1 181,215 + GFF153 0.65 -0.4 181,215 + GFF153 0.65 -1.0 181,215 + GFF153 0.65 +0.6 181,216 - GFF153 0.65 +1.7 181,216 - GFF153 0.65 +0.2 181,216 - GFF153 0.65 +0.2 181,216 - GFF153 0.65 -0.6 181,401 + GFF153 0.87 -0.9 181,401 + GFF153 0.87 -0.8 181,401 + GFF153 0.87 -0.1 181,402 - GFF153 0.87 +0.5 181,402 - GFF153 0.87 -0.5 181,402 - GFF153 0.87 -0.1 181,402 - GFF153 0.87 +0.4 181,402 - GFF153 0.87 +0.4 181,402 - GFF153 0.87 -0.1 181,402 - GFF153 0.87 +1.4 182,273 + GFF154 0.46 -0.6 182,273 + GFF154 0.46 -0.9 182,273 + GFF154 0.46 +0.4 182,373 - GFF154 0.52 -0.7 182,373 - GFF154 0.52 +0.7 182,373 - GFF154 0.52 +0.2
Or see this region's nucleotide sequence