Strain Fitness in Variovorax sp. SCN45 around GFF117

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF115 and GFF116 are separated by 3 nucleotidesGFF116 and GFF117 are separated by 188 nucleotidesGFF117 and GFF118 are separated by 13 nucleotides GFF115 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 143,390 to 144,055 GFF115 GFF116 - RidA/YER057c/UK114 superfamily, group 1, at 144,059 to 144,571 GFF116 GFF117 - no description, at 144,760 to 145,539 GFF117 GFF118 - putative membrane protein, at 145,553 to 146,578 GFF118 Position (kb) 144 145 146Strain fitness (log2 ratio) -2 -1 0 1 2 3at 143.827 kb on - strand, within GFF115at 143.827 kb on - strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.877 kb on + strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.878 kb on - strand, within GFF115at 143.977 kb on - strand, within GFF115at 143.977 kb on - strand, within GFF115at 144.019 kb on - strandat 144.048 kb on + strandat 144.048 kb on + strandat 144.333 kb on + strand, within GFF116at 144.333 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.417 kb on + strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.418 kb on - strand, within GFF116at 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.685 kb on + strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.686 kb on - strandat 144.712 kb on - strandat 144.872 kb on - strand, within GFF117at 145.120 kb on + strand, within GFF117at 145.120 kb on + strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.121 kb on - strand, within GFF117at 145.273 kb on + strand, within GFF117at 145.273 kb on + strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.274 kb on - strand, within GFF117at 145.367 kb on - strand, within GFF117at 145.367 kb on - strand, within GFF117at 145.498 kb on + strandat 145.499 kb on - strandat 145.499 kb on - strandat 145.499 kb on - strandat 145.531 kb on + strandat 145.532 kb on - strandat 145.532 kb on - strandat 145.643 kb on + strandat 145.644 kb on - strandat 145.674 kb on + strand, within GFF118at 145.674 kb on + strand, within GFF118at 145.675 kb on - strand, within GFF118at 145.675 kb on - strand, within GFF118at 145.675 kb on - strand, within GFF118at 145.675 kb on - strand, within GFF118at 145.675 kb on - strand, within GFF118at 145.761 kb on - strand, within GFF118at 145.926 kb on - strand, within GFF118at 145.953 kb on - strand, within GFF118at 145.995 kb on - strand, within GFF118at 145.995 kb on - strand, within GFF118at 145.995 kb on - strand, within GFF118at 146.204 kb on + strand, within GFF118at 146.204 kb on + strand, within GFF118at 146.204 kb on + strand, within GFF118at 146.204 kb on + strand, within GFF118at 146.204 kb on + strand, within GFF118at 146.205 kb on - strand, within GFF118at 146.205 kb on - strand, within GFF118at 146.205 kb on - strand, within GFF118at 146.210 kb on + strand, within GFF118at 146.210 kb on + strand, within GFF118at 146.210 kb on + strand, within GFF118at 146.211 kb on - strand, within GFF118at 146.211 kb on - strand, within GFF118at 146.300 kb on + strand, within GFF118at 146.400 kb on - strand, within GFF118at 146.411 kb on + strand, within GFF118at 146.411 kb on + strand, within GFF118at 146.418 kb on - strand, within GFF118

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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143,827 - GFF115 0.66 +1.1
143,827 - GFF115 0.66 +1.7
143,877 + GFF115 0.73 +0.1
143,877 + GFF115 0.73 +0.7
143,877 + GFF115 0.73 +0.8
143,877 + GFF115 0.73 +0.3
143,877 + GFF115 0.73 -0.6
143,877 + GFF115 0.73 -0.2
143,877 + GFF115 0.73 +0.6
143,878 - GFF115 0.73 +0.9
143,878 - GFF115 0.73 -0.6
143,878 - GFF115 0.73 +2.8
143,878 - GFF115 0.73 -0.3
143,977 - GFF115 0.88 +0.7
143,977 - GFF115 0.88 +0.3
144,019 - -0.6
144,048 + +0.7
144,048 + -0.0
144,333 + GFF116 0.53 -0.0
144,333 + GFF116 0.53 -0.2
144,417 + GFF116 0.70 +0.2
144,417 + GFF116 0.70 -0.3
144,417 + GFF116 0.70 +0.1
144,417 + GFF116 0.70 -0.4
144,417 + GFF116 0.70 +2.0
144,418 - GFF116 0.70 -0.8
144,418 - GFF116 0.70 -0.2
144,418 - GFF116 0.70 -0.8
144,418 - GFF116 0.70 -0.9
144,418 - GFF116 0.70 +0.1
144,685 + -0.1
144,685 + +0.3
144,685 + -0.6
144,685 + -0.1
144,685 + +1.0
144,685 + -0.1
144,685 + +0.2
144,686 - -0.7
144,686 - -0.6
144,686 - +0.6
144,686 - -0.4
144,686 - -0.3
144,686 - -1.8
144,686 - +0.5
144,686 - -1.3
144,712 - -1.0
144,872 - GFF117 0.14 -2.5
145,120 + GFF117 0.46 -0.6
145,120 + GFF117 0.46 -0.0
145,121 - GFF117 0.46 +0.5
145,121 - GFF117 0.46 -1.5
145,121 - GFF117 0.46 -0.2
145,273 + GFF117 0.66 +1.0
145,273 + GFF117 0.66 -0.1
145,274 - GFF117 0.66 -2.1
145,274 - GFF117 0.66 -0.3
145,274 - GFF117 0.66 -0.0
145,367 - GFF117 0.78 -1.0
145,367 - GFF117 0.78 -1.3
145,498 + -0.5
145,499 - -0.8
145,499 - -0.7
145,499 - -0.2
145,531 + +1.2
145,532 - -0.5
145,532 - +0.1
145,643 + +0.4
145,644 - -0.1
145,674 + GFF118 0.12 -0.0
145,674 + GFF118 0.12 -0.4
145,675 - GFF118 0.12 +1.0
145,675 - GFF118 0.12 +0.1
145,675 - GFF118 0.12 +1.4
145,675 - GFF118 0.12 -0.7
145,675 - GFF118 0.12 -1.3
145,761 - GFF118 0.20 -0.3
145,926 - GFF118 0.36 -0.6
145,953 - GFF118 0.39 +1.5
145,995 - GFF118 0.43 -0.4
145,995 - GFF118 0.43 -0.6
145,995 - GFF118 0.43 -1.2
146,204 + GFF118 0.63 -2.0
146,204 + GFF118 0.63 -0.2
146,204 + GFF118 0.63 -0.4
146,204 + GFF118 0.63 -0.8
146,204 + GFF118 0.63 +0.4
146,205 - GFF118 0.64 -0.6
146,205 - GFF118 0.64 -0.0
146,205 - GFF118 0.64 -0.9
146,210 + GFF118 0.64 -0.5
146,210 + GFF118 0.64 -0.0
146,210 + GFF118 0.64 -2.2
146,211 - GFF118 0.64 -0.6
146,211 - GFF118 0.64 -0.9
146,300 + GFF118 0.73 -1.8
146,400 - GFF118 0.83 -0.7
146,411 + GFF118 0.84 -1.3
146,411 + GFF118 0.84 -0.9
146,418 - GFF118 0.84 -0.2

Or see this region's nucleotide sequence