Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1162 and GFF1163 are separated by 81 nucleotides GFF1163 and GFF1164 are separated by 15 nucleotides GFF1164 and GFF1165 are separated by 63 nucleotides
GFF1162 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 477,221 to 478,045
GFF1162
GFF1163 - no description, at 478,127 to 478,240
GFF1163
GFF1164 - 2-isopropylmalate synthase (EC 2.3.3.13), at 478,256 to 479,959
GFF1164
GFF1165 - 2-isopropylmalate synthase (EC 2.3.3.13), at 480,023 to 481,561
GFF1165
Position (kb) 478
479
480 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 477.278 kb on + strand at 477.728 kb on + strand, within GFF1162 at 477.812 kb on + strand, within GFF1162 at 478.003 kb on + strand at 478.003 kb on + strand at 478.003 kb on + strand at 478.003 kb on + strand at 478.003 kb on + strand at 478.004 kb on - strand at 478.004 kb on - strand at 478.004 kb on - strand at 478.004 kb on - strand at 478.084 kb on + strand at 478.084 kb on + strand at 478.084 kb on + strand at 478.085 kb on - strand at 478.085 kb on - strand at 478.085 kb on - strand at 478.085 kb on - strand at 478.085 kb on - strand at 478.126 kb on - strand at 478.126 kb on - strand at 478.126 kb on - strand at 478.128 kb on + strand at 478.128 kb on + strand at 478.128 kb on + strand at 478.128 kb on + strand at 478.128 kb on + strand at 478.129 kb on - strand at 478.129 kb on - strand at 478.129 kb on - strand at 478.129 kb on - strand at 478.129 kb on - strand at 478.129 kb on - strand at 478.231 kb on + strand at 478.231 kb on + strand at 478.231 kb on + strand at 478.231 kb on + strand at 478.232 kb on - strand at 478.232 kb on - strand at 478.232 kb on - strand at 478.232 kb on - strand at 478.232 kb on - strand at 478.232 kb on - strand at 478.234 kb on + strand at 478.241 kb on + strand at 478.241 kb on + strand at 478.241 kb on + strand at 478.242 kb on - strand at 478.242 kb on - strand at 478.280 kb on + strand at 478.280 kb on + strand at 478.280 kb on + strand at 478.281 kb on - strand at 478.281 kb on - strand at 478.281 kb on - strand at 478.281 kb on - strand at 478.282 kb on - strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.315 kb on + strand at 478.316 kb on - strand at 478.316 kb on - strand at 478.316 kb on - strand at 478.316 kb on - strand at 478.637 kb on + strand, within GFF1164 at 478.637 kb on + strand, within GFF1164 at 478.638 kb on - strand, within GFF1164 at 478.638 kb on - strand, within GFF1164 at 478.638 kb on - strand, within GFF1164 at 478.638 kb on - strand, within GFF1164 at 478.711 kb on + strand, within GFF1164 at 478.775 kb on + strand, within GFF1164 at 478.775 kb on + strand, within GFF1164 at 478.776 kb on - strand, within GFF1164 at 478.776 kb on - strand, within GFF1164 at 478.824 kb on + strand, within GFF1164 at 478.824 kb on + strand, within GFF1164 at 478.915 kb on + strand, within GFF1164 at 478.916 kb on - strand, within GFF1164 at 478.916 kb on - strand, within GFF1164 at 478.993 kb on + strand, within GFF1164 at 478.993 kb on + strand, within GFF1164 at 479.126 kb on + strand, within GFF1164 at 479.126 kb on + strand, within GFF1164 at 479.126 kb on + strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.330 kb on + strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.417 kb on + strand, within GFF1164 at 479.417 kb on + strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.435 kb on + strand, within GFF1164 at 479.435 kb on + strand, within GFF1164 at 479.436 kb on - strand, within GFF1164 at 479.436 kb on - strand, within GFF1164 at 479.555 kb on + strand, within GFF1164 at 479.555 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.804 kb on + strand at 479.804 kb on + strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.902 kb on - strand at 480.040 kb on - strand at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.561 kb on - strand, within GFF1165 at 480.561 kb on - strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 477,278 + +2.5 477,728 + GFF1162 0.61 -1.5 477,812 + GFF1162 0.72 +0.2 478,003 + -0.8 478,003 + -0.4 478,003 + -1.2 478,003 + -1.5 478,003 + +0.9 478,004 - +0.4 478,004 - -0.2 478,004 - +0.6 478,004 - -0.7 478,084 + -0.2 478,084 + +0.1 478,084 + -1.0 478,085 - -0.7 478,085 - -1.1 478,085 - -1.3 478,085 - +0.3 478,085 - -0.3 478,126 - -0.9 478,126 - -0.0 478,126 - +0.4 478,128 + -1.1 478,128 + -0.2 478,128 + -0.4 478,128 + -0.5 478,128 + -0.7 478,129 - -0.3 478,129 - -0.5 478,129 - -0.9 478,129 - +0.6 478,129 - +0.9 478,129 - -0.4 478,231 + -1.0 478,231 + +0.1 478,231 + -1.0 478,231 + +0.1 478,232 - +0.6 478,232 - +0.5 478,232 - -1.1 478,232 - -1.1 478,232 - -0.3 478,232 - +0.6 478,234 + -0.2 478,241 + -0.2 478,241 + -0.3 478,241 + +0.3 478,242 - -0.2 478,242 - +1.5 478,280 + +0.8 478,280 + +0.5 478,280 + +0.5 478,281 - -0.2 478,281 - +0.1 478,281 - +0.2 478,281 - +1.2 478,282 - -0.6 478,315 + +0.6 478,315 + -0.8 478,315 + +0.3 478,315 + -0.7 478,315 + +0.7 478,315 + -1.4 478,315 + -0.2 478,315 + -0.5 478,315 + -1.6 478,315 + -0.1 478,316 - -0.5 478,316 - -2.0 478,316 - -0.4 478,316 - +0.0 478,637 + GFF1164 0.22 -0.0 478,637 + GFF1164 0.22 -1.5 478,638 - GFF1164 0.22 -0.1 478,638 - GFF1164 0.22 -0.5 478,638 - GFF1164 0.22 -1.2 478,638 - GFF1164 0.22 +0.6 478,711 + GFF1164 0.27 -0.4 478,775 + GFF1164 0.30 -0.5 478,775 + GFF1164 0.30 -0.4 478,776 - GFF1164 0.31 +0.7 478,776 - GFF1164 0.31 -0.3 478,824 + GFF1164 0.33 -1.3 478,824 + GFF1164 0.33 -0.2 478,915 + GFF1164 0.39 -0.1 478,916 - GFF1164 0.39 -1.1 478,916 - GFF1164 0.39 -0.7 478,993 + GFF1164 0.43 +1.0 478,993 + GFF1164 0.43 -1.4 479,126 + GFF1164 0.51 -1.3 479,126 + GFF1164 0.51 +0.2 479,126 + GFF1164 0.51 -0.5 479,127 - GFF1164 0.51 -0.7 479,127 - GFF1164 0.51 +0.6 479,127 - GFF1164 0.51 -0.5 479,228 + GFF1164 0.57 -1.1 479,228 + GFF1164 0.57 -1.2 479,228 + GFF1164 0.57 -0.6 479,228 + GFF1164 0.57 -0.1 479,228 + GFF1164 0.57 +0.7 479,228 + GFF1164 0.57 -0.7 479,228 + GFF1164 0.57 +1.3 479,228 + GFF1164 0.57 +0.3 479,229 - GFF1164 0.57 -0.1 479,229 - GFF1164 0.57 -0.7 479,229 - GFF1164 0.57 -0.7 479,229 - GFF1164 0.57 -0.0 479,229 - GFF1164 0.57 +0.1 479,246 + GFF1164 0.58 +0.4 479,246 + GFF1164 0.58 -0.2 479,246 + GFF1164 0.58 -0.3 479,246 + GFF1164 0.58 -0.7 479,246 + GFF1164 0.58 +0.9 479,247 - GFF1164 0.58 -0.5 479,247 - GFF1164 0.58 -1.1 479,247 - GFF1164 0.58 -1.0 479,247 - GFF1164 0.58 -0.3 479,247 - GFF1164 0.58 -0.3 479,330 + GFF1164 0.63 -0.1 479,331 - GFF1164 0.63 +0.2 479,331 - GFF1164 0.63 -0.5 479,331 - GFF1164 0.63 +0.5 479,331 - GFF1164 0.63 +0.1 479,331 - GFF1164 0.63 +0.1 479,366 + GFF1164 0.65 -1.0 479,366 + GFF1164 0.65 -0.3 479,366 + GFF1164 0.65 -0.7 479,366 + GFF1164 0.65 -1.0 479,367 - GFF1164 0.65 -1.0 479,367 - GFF1164 0.65 -1.7 479,367 - GFF1164 0.65 -1.0 479,417 + GFF1164 0.68 -0.1 479,417 + GFF1164 0.68 -1.3 479,418 - GFF1164 0.68 -1.3 479,418 - GFF1164 0.68 +0.2 479,418 - GFF1164 0.68 -0.6 479,435 + GFF1164 0.69 -0.2 479,435 + GFF1164 0.69 -0.1 479,436 - GFF1164 0.69 -1.0 479,436 - GFF1164 0.69 -0.8 479,555 + GFF1164 0.76 +0.2 479,555 + GFF1164 0.76 -0.5 479,756 + GFF1164 0.88 +0.1 479,756 + GFF1164 0.88 +1.0 479,756 + GFF1164 0.88 -0.4 479,757 - GFF1164 0.88 +2.2 479,757 - GFF1164 0.88 +0.3 479,757 - GFF1164 0.88 +0.8 479,757 - GFF1164 0.88 +0.2 479,757 - GFF1164 0.88 -0.1 479,757 - GFF1164 0.88 -0.5 479,757 - GFF1164 0.88 +0.5 479,804 + -0.6 479,804 + +0.9 479,805 - +0.5 479,805 - -0.5 479,805 - -0.5 479,805 - -0.3 479,901 + -0.5 479,901 + -0.2 479,901 + +0.9 479,901 + -0.7 479,901 + +0.0 479,901 + +0.3 479,902 - -0.6 480,040 - +0.6 480,560 + GFF1165 0.35 +0.0 480,560 + GFF1165 0.35 -0.4 480,560 + GFF1165 0.35 -0.5 480,560 + GFF1165 0.35 +0.2 480,560 + GFF1165 0.35 +0.4 480,561 - GFF1165 0.35 +0.2 480,561 - GFF1165 0.35 -0.5 480,785 + GFF1165 0.50 -0.4 480,785 + GFF1165 0.50 +0.7 480,785 + GFF1165 0.50 -0.5 480,785 + GFF1165 0.50 +0.1 480,785 + GFF1165 0.50 -0.3 480,785 + GFF1165 0.50 +0.3 480,786 - GFF1165 0.50 +0.2 480,786 - GFF1165 0.50 -0.1 480,786 - GFF1165 0.50 +2.8 480,786 - GFF1165 0.50 -0.2 480,956 + GFF1165 0.61 -0.4 480,956 + GFF1165 0.61 +0.9 480,956 + GFF1165 0.61 -0.1 480,957 - GFF1165 0.61 +0.5 480,957 - GFF1165 0.61 -0.5 480,957 - GFF1165 0.61 +0.3 480,957 - GFF1165 0.61 -0.8
Or see this region's nucleotide sequence