Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF996 and GFF997 are separated by 5 nucleotides GFF997 and GFF998 are separated by 7 nucleotides
GFF996 - Histidyl-tRNA synthetase (EC 6.1.1.21), at 315,247 to 316,557
GFF996
GFF997 - UPF0070 protein YfgM, at 316,563 to 317,255
GFF997
GFF998 - Outer membrane beta-barrel assembly protein BamB, at 317,263 to 318,405
GFF998
Position (kb) 316
317
318 Strain fitness (log2 ratio) -2
-1
0
1 at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.532 kb on + strand at 316.533 kb on - strand at 316.533 kb on - strand at 316.533 kb on - strand at 316.830 kb on + strand, within GFF997 at 316.830 kb on + strand, within GFF997 at 316.830 kb on + strand, within GFF997 at 316.941 kb on + strand, within GFF997 at 316.941 kb on + strand, within GFF997 at 316.941 kb on + strand, within GFF997 at 316.941 kb on + strand, within GFF997 at 316.941 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.076 kb on + strand, within GFF997 at 317.115 kb on + strand, within GFF997 at 317.154 kb on + strand, within GFF997
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 316,532 + +0.0 316,532 + -0.0 316,532 + +0.5 316,532 + +0.3 316,532 + -0.2 316,532 + -2.0 316,532 + -0.3 316,532 + -0.3 316,532 + -0.8 316,532 + +0.2 316,533 - +1.4 316,533 - -0.6 316,533 - -0.1 316,830 + GFF997 0.39 -1.0 316,830 + GFF997 0.39 -0.8 316,830 + GFF997 0.39 -1.0 316,941 + GFF997 0.55 -1.8 316,941 + GFF997 0.55 -2.5 316,941 + GFF997 0.55 +0.8 316,941 + GFF997 0.55 +0.1 316,941 + GFF997 0.55 +1.4 317,076 + GFF997 0.74 +0.3 317,076 + GFF997 0.74 +0.6 317,076 + GFF997 0.74 -1.2 317,076 + GFF997 0.74 +0.4 317,076 + GFF997 0.74 +1.0 317,076 + GFF997 0.74 -0.6 317,076 + GFF997 0.74 -1.7 317,076 + GFF997 0.74 -2.5 317,076 + GFF997 0.74 -0.2 317,076 + GFF997 0.74 +0.6 317,076 + GFF997 0.74 -2.5 317,076 + GFF997 0.74 -1.0 317,076 + GFF997 0.74 +0.4 317,115 + GFF997 0.80 +0.6 317,154 + GFF997 0.85 -1.3
Or see this region's nucleotide sequence