Strain Fitness in Variovorax sp. SCN45 around GFF939

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF937 and GFF938 overlap by 4 nucleotidesGFF938 and GFF939 overlap by 11 nucleotidesGFF939 and GFF940 are separated by 118 nucleotides GFF937 - Biotin synthase (EC 2.8.1.6), at 251,356 to 252,390 GFF937 GFF938 - Methylmalonyl-CoA epimerase (EC 5.1.99.1), at 252,387 to 252,878 GFF938 GFF939 - Transcriptional regulator, AsnC family, at 252,868 to 253,365 GFF939 GFF940 - Permease of the drug/metabolite transporter (DMT) superfamily, at 253,484 to 254,392 GFF940 Position (kb) 252 253 254Strain fitness (log2 ratio) -1 0 1at 251.894 kb on - strand, within GFF937at 251.894 kb on - strand, within GFF937at 251.894 kb on - strand, within GFF937at 251.896 kb on + strand, within GFF937at 251.896 kb on + strand, within GFF937at 251.896 kb on + strand, within GFF937at 251.896 kb on + strand, within GFF937at 251.897 kb on - strand, within GFF937at 251.897 kb on - strand, within GFF937at 251.897 kb on - strand, within GFF937at 251.897 kb on - strand, within GFF937at 251.923 kb on + strand, within GFF937at 251.923 kb on + strand, within GFF937at 251.924 kb on - strand, within GFF937at 251.924 kb on - strand, within GFF937at 251.924 kb on - strand, within GFF937at 251.924 kb on - strand, within GFF937at 252.271 kb on + strand, within GFF937at 252.272 kb on - strand, within GFF937at 252.869 kb on + strandat 252.869 kb on + strandat 252.870 kb on - strandat 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.070 kb on + strand, within GFF939at 253.071 kb on - strand, within GFF939at 253.161 kb on - strand, within GFF939at 253.161 kb on - strand, within GFF939at 253.161 kb on - strand, within GFF939at 253.376 kb on + strandat 253.440 kb on + strandat 253.441 kb on - strandat 253.441 kb on - strandat 253.592 kb on + strand, within GFF940at 253.592 kb on + strand, within GFF940at 253.592 kb on + strand, within GFF940at 253.592 kb on + strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.593 kb on - strand, within GFF940at 253.607 kb on + strand, within GFF940at 253.607 kb on + strand, within GFF940at 253.607 kb on + strand, within GFF940at 253.607 kb on + strand, within GFF940at 253.608 kb on - strand, within GFF940at 253.608 kb on - strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.171 kb on + strand, within GFF940at 254.172 kb on - strand, within GFF940at 254.172 kb on - strand, within GFF940at 254.172 kb on - strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.210 kb on + strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.211 kb on - strand, within GFF940at 254.255 kb on + strand, within GFF940at 254.255 kb on + strand, within GFF940at 254.255 kb on + strand, within GFF940at 254.255 kb on + strand, within GFF940at 254.255 kb on + strand, within GFF940at 254.256 kb on - strand, within GFF940at 254.256 kb on - strand, within GFF940at 254.256 kb on - strand, within GFF940at 254.256 kb on - strand, within GFF940at 254.256 kb on - strand, within GFF940at 254.261 kb on + strand, within GFF940at 254.334 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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251,894 - GFF937 0.52 -1.4
251,894 - GFF937 0.52 -1.0
251,894 - GFF937 0.52 -1.5
251,896 + GFF937 0.52 -1.2
251,896 + GFF937 0.52 -1.4
251,896 + GFF937 0.52 -1.3
251,896 + GFF937 0.52 -0.7
251,897 - GFF937 0.52 +0.3
251,897 - GFF937 0.52 -1.6
251,897 - GFF937 0.52 -0.8
251,897 - GFF937 0.52 -0.9
251,923 + GFF937 0.55 -0.7
251,923 + GFF937 0.55 -0.6
251,924 - GFF937 0.55 -0.6
251,924 - GFF937 0.55 -0.5
251,924 - GFF937 0.55 -0.6
251,924 - GFF937 0.55 -0.2
252,271 + GFF937 0.88 -0.2
252,272 - GFF937 0.89 -0.5
252,869 + +0.5
252,869 + +0.3
252,870 - +0.0
253,070 + GFF939 0.41 -0.6
253,070 + GFF939 0.41 -0.5
253,070 + GFF939 0.41 -0.7
253,070 + GFF939 0.41 +1.4
253,070 + GFF939 0.41 +1.0
253,070 + GFF939 0.41 +0.5
253,070 + GFF939 0.41 -1.0
253,070 + GFF939 0.41 +0.3
253,070 + GFF939 0.41 -1.2
253,070 + GFF939 0.41 -1.0
253,070 + GFF939 0.41 +0.4
253,071 - GFF939 0.41 +0.2
253,161 - GFF939 0.59 -0.0
253,161 - GFF939 0.59 +0.0
253,161 - GFF939 0.59 +0.3
253,376 + -0.8
253,440 + +0.1
253,441 - +1.4
253,441 - -1.0
253,592 + GFF940 0.12 +1.2
253,592 + GFF940 0.12 +0.3
253,592 + GFF940 0.12 -0.1
253,592 + GFF940 0.12 -0.5
253,593 - GFF940 0.12 +0.8
253,593 - GFF940 0.12 -0.6
253,593 - GFF940 0.12 -0.1
253,593 - GFF940 0.12 +1.4
253,593 - GFF940 0.12 -1.1
253,593 - GFF940 0.12 -0.1
253,607 + GFF940 0.14 +0.1
253,607 + GFF940 0.14 +1.5
253,607 + GFF940 0.14 +0.2
253,607 + GFF940 0.14 +0.1
253,608 - GFF940 0.14 +0.0
253,608 - GFF940 0.14 -1.1
254,171 + GFF940 0.76 +0.7
254,171 + GFF940 0.76 -0.6
254,171 + GFF940 0.76 -0.3
254,171 + GFF940 0.76 -0.0
254,171 + GFF940 0.76 +0.5
254,171 + GFF940 0.76 -0.1
254,172 - GFF940 0.76 -0.5
254,172 - GFF940 0.76 -0.6
254,172 - GFF940 0.76 -0.5
254,210 + GFF940 0.80 -0.1
254,210 + GFF940 0.80 -0.4
254,210 + GFF940 0.80 +0.8
254,210 + GFF940 0.80 -1.0
254,210 + GFF940 0.80 +1.6
254,210 + GFF940 0.80 -0.5
254,210 + GFF940 0.80 -0.3
254,210 + GFF940 0.80 -0.4
254,210 + GFF940 0.80 -0.4
254,210 + GFF940 0.80 -0.7
254,211 - GFF940 0.80 -0.2
254,211 - GFF940 0.80 -1.4
254,211 - GFF940 0.80 +0.7
254,211 - GFF940 0.80 +0.4
254,211 - GFF940 0.80 -0.2
254,211 - GFF940 0.80 -0.2
254,211 - GFF940 0.80 -0.5
254,211 - GFF940 0.80 +0.6
254,211 - GFF940 0.80 +0.5
254,211 - GFF940 0.80 +0.4
254,211 - GFF940 0.80 -0.1
254,211 - GFF940 0.80 -0.0
254,255 + GFF940 0.85 -0.3
254,255 + GFF940 0.85 -0.6
254,255 + GFF940 0.85 -0.2
254,255 + GFF940 0.85 -0.8
254,255 + GFF940 0.85 -0.6
254,256 - GFF940 0.85 +0.3
254,256 - GFF940 0.85 +0.7
254,256 - GFF940 0.85 -0.4
254,256 - GFF940 0.85 +0.2
254,256 - GFF940 0.85 +0.9
254,261 + GFF940 0.85 +0.2
254,334 - +0.2

Or see this region's nucleotide sequence