Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF787 and GFF788 are separated by 21 nucleotides GFF788 and GFF789 are separated by 1 nucleotides GFF789 and GFF790 are separated by 179 nucleotides GFF790 and GFF791 are separated by 7 nucleotides
GFF787 - hypothetical protein, at 80,455 to 81,186
GFF787
GFF788 - hypothetical protein, at 81,208 to 81,681
GFF788
GFF789 - protease Do, at 81,683 to 83,071
GFF789
GFF790 - hypothetical protein, at 83,251 to 83,922
GFF790
GFF791 - RNA polymerase sigma factor RpoE, at 83,930 to 84,466
GFF791
Position (kb) 81
82
83
84 Strain fitness (log2 ratio) -2
-1
0
1 at 80.728 kb on + strand, within GFF787 at 80.728 kb on + strand, within GFF787 at 80.729 kb on - strand, within GFF787 at 80.729 kb on - strand, within GFF787 at 80.797 kb on + strand, within GFF787 at 80.797 kb on + strand, within GFF787 at 80.798 kb on - strand, within GFF787 at 80.798 kb on - strand, within GFF787 at 80.798 kb on - strand, within GFF787 at 81.195 kb on + strand at 81.222 kb on + strand at 81.231 kb on + strand at 81.231 kb on + strand at 81.231 kb on + strand at 81.361 kb on + strand, within GFF788 at 81.361 kb on + strand, within GFF788 at 81.362 kb on - strand, within GFF788 at 81.419 kb on + strand, within GFF788 at 81.810 kb on - strand at 82.185 kb on - strand, within GFF789 at 82.199 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.253 kb on + strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.254 kb on - strand, within GFF789 at 82.355 kb on + strand, within GFF789 at 82.355 kb on + strand, within GFF789 at 82.355 kb on + strand, within GFF789 at 82.355 kb on + strand, within GFF789 at 82.355 kb on + strand, within GFF789 at 82.356 kb on - strand, within GFF789 at 82.356 kb on - strand, within GFF789 at 82.356 kb on - strand, within GFF789 at 82.374 kb on - strand, within GFF789 at 82.545 kb on - strand, within GFF789 at 82.545 kb on - strand, within GFF789 at 82.545 kb on - strand, within GFF789 at 82.545 kb on - strand, within GFF789 at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.136 kb on + strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.137 kb on - strand at 83.174 kb on - strand at 83.365 kb on + strand, within GFF790 at 83.365 kb on + strand, within GFF790 at 83.503 kb on + strand, within GFF790 at 83.503 kb on + strand, within GFF790 at 83.503 kb on + strand, within GFF790 at 83.504 kb on - strand, within GFF790 at 83.504 kb on - strand, within GFF790 at 83.632 kb on + strand, within GFF790 at 83.633 kb on - strand, within GFF790 at 83.633 kb on - strand, within GFF790 at 83.726 kb on + strand, within GFF790 at 83.726 kb on + strand, within GFF790 at 83.727 kb on - strand, within GFF790 at 83.727 kb on - strand, within GFF790 at 83.727 kb on - strand, within GFF790 at 83.978 kb on + strand at 83.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 80,728 + GFF787 0.37 -1.2 80,728 + GFF787 0.37 -0.5 80,729 - GFF787 0.37 +0.9 80,729 - GFF787 0.37 -0.0 80,797 + GFF787 0.47 -1.0 80,797 + GFF787 0.47 -0.7 80,798 - GFF787 0.47 -2.1 80,798 - GFF787 0.47 -0.0 80,798 - GFF787 0.47 -0.4 81,195 + -0.1 81,222 + +0.4 81,231 + -0.6 81,231 + -0.4 81,231 + -0.5 81,361 + GFF788 0.32 -1.2 81,361 + GFF788 0.32 +0.8 81,362 - GFF788 0.32 +0.4 81,419 + GFF788 0.45 +0.4 81,810 - +0.8 82,185 - GFF789 0.36 -0.8 82,199 + GFF789 0.37 +0.3 82,253 + GFF789 0.41 +1.0 82,253 + GFF789 0.41 -1.6 82,253 + GFF789 0.41 +0.1 82,253 + GFF789 0.41 -0.4 82,253 + GFF789 0.41 -2.0 82,253 + GFF789 0.41 +0.4 82,253 + GFF789 0.41 +0.9 82,254 - GFF789 0.41 -1.4 82,254 - GFF789 0.41 -0.6 82,254 - GFF789 0.41 -1.2 82,254 - GFF789 0.41 +0.9 82,254 - GFF789 0.41 -1.2 82,254 - GFF789 0.41 -0.2 82,355 + GFF789 0.48 -0.3 82,355 + GFF789 0.48 +0.3 82,355 + GFF789 0.48 -0.0 82,355 + GFF789 0.48 -0.3 82,355 + GFF789 0.48 +0.3 82,356 - GFF789 0.48 -0.1 82,356 - GFF789 0.48 -0.1 82,356 - GFF789 0.48 -0.1 82,374 - GFF789 0.50 -0.2 82,545 - GFF789 0.62 -0.4 82,545 - GFF789 0.62 +0.5 82,545 - GFF789 0.62 +1.1 82,545 - GFF789 0.62 -0.2 83,136 + +0.7 83,136 + -0.6 83,136 + -1.9 83,136 + -0.4 83,136 + -2.4 83,136 + -0.1 83,136 + +0.2 83,136 + +0.1 83,137 - +1.1 83,137 - +0.1 83,137 - -2.2 83,137 - -1.6 83,137 - +0.0 83,137 - -0.7 83,137 - -0.1 83,137 - +0.2 83,174 - +1.5 83,365 + GFF790 0.17 -1.5 83,365 + GFF790 0.17 -0.6 83,503 + GFF790 0.38 -1.8 83,503 + GFF790 0.38 -0.0 83,503 + GFF790 0.38 +1.4 83,504 - GFF790 0.38 +0.3 83,504 - GFF790 0.38 -0.6 83,632 + GFF790 0.57 -0.3 83,633 - GFF790 0.57 +0.6 83,633 - GFF790 0.57 -0.5 83,726 + GFF790 0.71 +0.2 83,726 + GFF790 0.71 -0.2 83,727 - GFF790 0.71 -0.5 83,727 - GFF790 0.71 -0.6 83,727 - GFF790 0.71 +0.6 83,978 + -0.2 83,979 - +0.8
Or see this region's nucleotide sequence