Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt GFF7115 and GFF7116 are separated by 16 nucleotides GFF7116 and GFF7117 overlap by 4 nucleotides GFF7117 and GFF7118 overlap by 4 nucleotides GFF7118 and GFF7119 are separated by 6 nucleotides  
        
        GFF7115 - hypothetical protein, at 33,663 to 34,481 
         
        GFF7115 
         
        
        GFF7116 - 2,3-dihydroxybiphenyl 1,2-dioxygenase (EC 1.13.11.39), at 34,498 to 35,412 
         
        GFF7116 
         
        
        GFF7117 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 35,409 to 36,239 
         
        GFF7117 
         
        
        GFF7118 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 36,236 to 37,177 
         
        GFF7118 
         
        
        GFF7119 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 37,184 to 37,660 
         
        GFF7119 
         Position (kb)  
35 
 
36 
 
37 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.539 kb on - strand at 34.550 kb on + strand at 34.551 kb on - strand at 34.551 kb on - strand at 34.553 kb on + strand at 34.553 kb on + strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.863 kb on - strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 35.014 kb on + strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.046 kb on + strand, within GFF7116 at 35.047 kb on - strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.196 kb on - strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.357 kb on + strand at 35.357 kb on + strand at 35.358 kb on - strand at 35.582 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.797 kb on + strand, within GFF7117 at 35.971 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 36.004 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.109 kb on + strand, within GFF7117 at 36.175 kb on + strand at 36.175 kb on + strand at 36.175 kb on + strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.187 kb on - strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.921 kb on - strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 37.032 kb on + strand, within GFF7118 at 37.185 kb on + strand at 37.185 kb on + strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL             remove   34,535   +        +0.4   34,535   +        -0.6   34,535   +        +0.4   34,535   +        +0.4   34,535   +        +0.6   34,535   +        +1.2   34,536   -        +1.8   34,536   -        +0.1   34,536   -        -0.5   34,536   -        -0.7   34,538   +        -0.3   34,538   +        +0.5   34,538   +        -1.0   34,538   +        -1.0   34,539   -        -0.6   34,550   +        -0.2   34,551   -        +0.1   34,551   -        +0.4   34,553   +        -1.2   34,553   +        -0.3   34,554   -        +0.9   34,554   -        +2.5   34,554   -        +1.2   34,554   -        -0.7   34,625   +    GFF7116   0.14  -0.1   34,625   +    GFF7116   0.14  +0.5   34,625   +    GFF7116   0.14  -2.0   34,626   -    GFF7116   0.14  -0.2   34,626   -    GFF7116   0.14  +0.1   34,626   -    GFF7116   0.14  -0.5   34,678   +    GFF7116   0.20  +0.2   34,678   +    GFF7116   0.20  -0.5   34,678   +    GFF7116   0.20  -0.8   34,679   -    GFF7116   0.20  +0.1   34,679   -    GFF7116   0.20  -0.9   34,679   -    GFF7116   0.20  -1.0   34,679   -    GFF7116   0.20  +0.1   34,679   -    GFF7116   0.20  -0.3   34,679   -    GFF7116   0.20  -0.8   34,733   +    GFF7116   0.26  +0.4   34,733   +    GFF7116   0.26  +0.2   34,733   +    GFF7116   0.26  -0.5   34,734   -    GFF7116   0.26  +1.2   34,734   -    GFF7116   0.26  +0.1   34,734   -    GFF7116   0.26  -0.0   34,734   -    GFF7116   0.26  -0.2   34,789   +    GFF7116   0.32  -0.7   34,789   +    GFF7116   0.32  -0.2   34,789   +    GFF7116   0.32  +0.3   34,790   -    GFF7116   0.32  +0.8   34,790   -    GFF7116   0.32  +0.2   34,863   -    GFF7116   0.40  -1.4   34,895   +    GFF7116   0.43  -0.9   34,895   +    GFF7116   0.43  +0.3   34,895   +    GFF7116   0.43  -0.0   34,895   +    GFF7116   0.43  -0.9   34,896   -    GFF7116   0.43  +0.1   34,896   -    GFF7116   0.43  +0.2   34,896   -    GFF7116   0.43  +0.2   35,014   +    GFF7116   0.56  -0.1   35,015   -    GFF7116   0.57  -0.7   35,015   -    GFF7116   0.57  +0.2   35,015   -    GFF7116   0.57  -0.2   35,028   -    GFF7116   0.58  +0.4   35,028   -    GFF7116   0.58  +0.1   35,028   -    GFF7116   0.58  -0.3   35,028   -    GFF7116   0.58  +0.9   35,028   -    GFF7116   0.58  +0.2   35,028   -    GFF7116   0.58  +0.2   35,046   +    GFF7116   0.60  -0.7   35,047   -    GFF7116   0.60  +0.2   35,154   +    GFF7116   0.72  +0.6   35,154   +    GFF7116   0.72  -0.6   35,154   +    GFF7116   0.72  -0.1   35,154   +    GFF7116   0.72  -0.6   35,155   -    GFF7116   0.72  +1.2   35,155   -    GFF7116   0.72  -0.8   35,155   -    GFF7116   0.72  -0.0   35,155   -    GFF7116   0.72  +1.2   35,155   -    GFF7116   0.72  +0.3   35,155   -    GFF7116   0.72  +0.1   35,195   +    GFF7116   0.76  -0.8   35,195   +    GFF7116   0.76  -0.3   35,196   -    GFF7116   0.76  -1.2   35,200   +    GFF7116   0.77  -0.6   35,200   +    GFF7116   0.77  +1.0   35,200   +    GFF7116   0.77  +0.3   35,200   +    GFF7116   0.77  -0.6   35,200   +    GFF7116   0.77  -0.9   35,200   +    GFF7116   0.77  -0.2   35,200   +    GFF7116   0.77  -0.9   35,200   +    GFF7116   0.77  +0.6   35,200   +    GFF7116   0.77  +0.6   35,200   +    GFF7116   0.77  -0.2   35,200   +    GFF7116   0.77  +0.4   35,200   +    GFF7116   0.77  +0.5   35,200   +    GFF7116   0.77  -0.5   35,200   +    GFF7116   0.77  -0.3   35,201   -    GFF7116   0.77  +1.0   35,201   -    GFF7116   0.77  -0.5   35,201   -    GFF7116   0.77  -0.8   35,201   -    GFF7116   0.77  -1.0   35,201   -    GFF7116   0.77  +0.0   35,201   -    GFF7116   0.77  -0.1   35,201   -    GFF7116   0.77  +0.1   35,201   -    GFF7116   0.77  +1.0   35,201   -    GFF7116   0.77  +0.3   35,201   -    GFF7116   0.77  -0.1   35,201   -    GFF7116   0.77  -0.1   35,201   -    GFF7116   0.77  -0.5   35,201   -    GFF7116   0.77  -0.5   35,201   -    GFF7116   0.77  +1.6   35,201   -    GFF7116   0.77  +0.5   35,201   -    GFF7116   0.77  -0.2   35,201   -    GFF7116   0.77  +0.3   35,201   -    GFF7116   0.77  +0.3   35,201   -    GFF7116   0.77  +1.1   35,201   -    GFF7116   0.77  +0.2   35,315   +    GFF7116   0.89  -0.4   35,315   +    GFF7116   0.89  +0.3   35,316   -    GFF7116   0.89  +0.6   35,316   -    GFF7116   0.89  +0.3   35,316   -    GFF7116   0.89  +1.1   35,316   -    GFF7116   0.89  -0.8   35,357   +        -0.5   35,357   +        -0.5   35,358   -        +0.1   35,582   +    GFF7117   0.21  -0.4   35,583   +    GFF7117   0.21  +1.4   35,583   +    GFF7117   0.21  -0.0   35,583   +    GFF7117   0.21  -0.6   35,583   +    GFF7117   0.21  +0.3   35,583   +    GFF7117   0.21  -0.4   35,583   +    GFF7117   0.21  +0.3   35,584   -    GFF7117   0.21  +1.3   35,584   -    GFF7117   0.21  -1.7   35,584   -    GFF7117   0.21  -0.8   35,584   -    GFF7117   0.21  -0.0   35,584   -    GFF7117   0.21  -0.0   35,584   -    GFF7117   0.21  -0.4   35,584   -    GFF7117   0.21  +0.2   35,584   -    GFF7117   0.21  +0.3   35,584   -    GFF7117   0.21  -1.5   35,584   -    GFF7117   0.21  -0.8   35,584   -    GFF7117   0.21  +1.8   35,771   +    GFF7117   0.44  +1.0   35,771   +    GFF7117   0.44  +0.3   35,771   +    GFF7117   0.44  -0.5   35,771   +    GFF7117   0.44  -0.7   35,771   +    GFF7117   0.44  -1.6   35,771   +    GFF7117   0.44  -0.4   35,771   +    GFF7117   0.44  +0.8   35,772   -    GFF7117   0.44  -0.3   35,772   -    GFF7117   0.44  +0.2   35,772   -    GFF7117   0.44  -1.4   35,772   -    GFF7117   0.44  +0.2   35,797   +    GFF7117   0.47  -0.3   35,971   +    GFF7117   0.68  -0.3   35,992   +    GFF7117   0.70  +0.2   35,992   +    GFF7117   0.70  +0.3   35,992   +    GFF7117   0.70  +1.0   35,992   +    GFF7117   0.70  -0.4   35,993   -    GFF7117   0.70  +0.4   35,993   -    GFF7117   0.70  +0.1   36,004   +    GFF7117   0.72  -0.4   36,094   +    GFF7117   0.82  +0.2   36,094   +    GFF7117   0.82  -0.4   36,095   -    GFF7117   0.83  +0.9   36,095   -    GFF7117   0.83  +0.3   36,095   -    GFF7117   0.83  +0.3   36,095   -    GFF7117   0.83  +1.1   36,109   +    GFF7117   0.84  -1.4   36,175   +        -1.5   36,175   +        +0.1   36,175   +        +0.5   36,176   -        -0.3   36,176   -        -1.6   36,176   -        +0.4   36,176   -        -0.6   36,186   +        +0.8   36,186   +        -0.5   36,186   +        -0.8   36,186   +        -0.2   36,187   -        +0.8   36,230   +        -1.7   36,230   +        +0.9   36,230   +        +0.6   36,230   +        -0.6   36,230   +        -0.4   36,230   +        -2.0   36,231   -        +0.5   36,231   -        -0.7   36,231   -        -0.7   36,447   +    GFF7118   0.22  +0.9   36,447   +    GFF7118   0.22  +0.5   36,447   +    GFF7118   0.22  +2.5   36,448   -    GFF7118   0.23  -0.4   36,448   -    GFF7118   0.23  +0.1   36,448   -    GFF7118   0.23  -1.4   36,921   -    GFF7118   0.73  -1.5   36,924   +    GFF7118   0.73  -0.7   36,924   +    GFF7118   0.73  +0.4   36,924   +    GFF7118   0.73  +0.3   36,925   -    GFF7118   0.73  -0.5   36,925   -    GFF7118   0.73  +1.8   36,925   -    GFF7118   0.73  -0.3   36,925   -    GFF7118   0.73  -0.5   36,925   -    GFF7118   0.73  -1.2   36,925   -    GFF7118   0.73  -0.4   36,925   -    GFF7118   0.73  -1.0   36,925   -    GFF7118   0.73  -0.3   36,925   -    GFF7118   0.73  +0.1   36,972   +    GFF7118   0.78  +0.2   36,972   +    GFF7118   0.78  +0.3   36,972   +    GFF7118   0.78  +0.1   36,972   +    GFF7118   0.78  +1.5   36,973   -    GFF7118   0.78  -0.5   36,973   -    GFF7118   0.78  +0.2   37,032   +    GFF7118   0.85  -0.5   37,185   +        +2.2   37,185   +        -1.4 
 
Or see this region's nucleotide sequence