Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7011 and GFF7012 overlap by 8 nucleotides GFF7012 and GFF7013 are separated by 53 nucleotides GFF7013 and GFF7014 are separated by 177 nucleotides
GFF7011 - Taurine ABC transporter, permease protein TauC, at 26,614 to 27,510
GFF7011
GFF7012 - Taurine ABC transporter, ATP-binding protein TauB, at 27,503 to 28,291
GFF7012
GFF7013 - Taurine ABC transporter, substrate-binding protein TauA, at 28,345 to 29,355
GFF7013
GFF7014 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 29,533 to 31,068
GFF7014
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-1
0
1 at 27.609 kb on + strand, within GFF7012 at 27.609 kb on + strand, within GFF7012 at 27.650 kb on - strand, within GFF7012 at 27.650 kb on - strand, within GFF7012 at 27.650 kb on - strand, within GFF7012 at 27.735 kb on + strand, within GFF7012 at 27.894 kb on + strand, within GFF7012 at 27.912 kb on + strand, within GFF7012 at 28.255 kb on - strand at 28.255 kb on - strand at 28.346 kb on + strand at 28.346 kb on + strand at 28.347 kb on - strand at 28.347 kb on - strand at 28.370 kb on + strand at 28.370 kb on + strand at 28.395 kb on - strand at 28.520 kb on + strand, within GFF7013 at 28.520 kb on + strand, within GFF7013 at 28.625 kb on + strand, within GFF7013 at 28.626 kb on - strand, within GFF7013 at 28.626 kb on - strand, within GFF7013 at 28.925 kb on + strand, within GFF7013 at 28.926 kb on - strand, within GFF7013 at 29.398 kb on - strand at 29.418 kb on - strand at 29.458 kb on + strand at 29.458 kb on + strand at 29.931 kb on - strand, within GFF7014 at 30.143 kb on + strand, within GFF7014
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 27,609 + GFF7012 0.13 -1.0 27,609 + GFF7012 0.13 +1.0 27,650 - GFF7012 0.19 -0.0 27,650 - GFF7012 0.19 +1.4 27,650 - GFF7012 0.19 -1.0 27,735 + GFF7012 0.29 -0.1 27,894 + GFF7012 0.50 +0.8 27,912 + GFF7012 0.52 -0.4 28,255 - -0.8 28,255 - -1.7 28,346 + -1.2 28,346 + -0.7 28,347 - +0.8 28,347 - -0.0 28,370 + +0.5 28,370 + +0.0 28,395 - -1.6 28,520 + GFF7013 0.17 +0.1 28,520 + GFF7013 0.17 -0.4 28,625 + GFF7013 0.28 -0.7 28,626 - GFF7013 0.28 -1.3 28,626 - GFF7013 0.28 -0.8 28,925 + GFF7013 0.57 -0.8 28,926 - GFF7013 0.57 -0.3 29,398 - +0.8 29,418 - -0.1 29,458 + -0.3 29,458 + -0.2 29,931 - GFF7014 0.26 -0.9 30,143 + GFF7014 0.40 +0.3
Or see this region's nucleotide sequence