Strain Fitness in Variovorax sp. SCN45 around GFF6586

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6585 and GFF6586 overlap by 4 nucleotidesGFF6586 and GFF6587 are separated by 30 nucleotidesGFF6587 and GFF6588 are separated by 85 nucleotides GFF6585 - D-glycerate 2-kinase (EC 2.7.1.165), at 26,854 to 28,179 GFF6585 GFF6586 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 28,176 to 29,084 GFF6586 GFF6587 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 29,115 to 29,894 GFF6587 GFF6588 - Glyoxylate carboligase (EC 4.1.1.47), at 29,980 to 31,770 GFF6588 Position (kb) 28 29 30Strain fitness (log2 ratio) -2 -1 0 1at 27.210 kb on + strand, within GFF6585at 27.519 kb on - strand, within GFF6585at 27.849 kb on + strand, within GFF6585at 27.951 kb on - strand, within GFF6585at 27.967 kb on + strand, within GFF6585at 27.968 kb on - strand, within GFF6585at 27.968 kb on - strand, within GFF6585at 27.996 kb on - strand, within GFF6585at 28.069 kb on + strandat 28.641 kb on + strand, within GFF6586at 28.642 kb on - strand, within GFF6586at 28.723 kb on + strand, within GFF6586at 28.723 kb on + strand, within GFF6586at 28.979 kb on - strand, within GFF6586at 28.979 kb on - strand, within GFF6586at 29.086 kb on + strandat 29.086 kb on + strandat 29.086 kb on + strandat 29.086 kb on + strandat 29.087 kb on - strandat 29.087 kb on - strandat 29.087 kb on - strandat 29.087 kb on - strandat 29.087 kb on - strandat 29.116 kb on + strandat 29.117 kb on - strandat 29.173 kb on + strandat 29.174 kb on - strandat 29.309 kb on - strand, within GFF6587at 29.309 kb on - strand, within GFF6587at 29.309 kb on - strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.356 kb on + strand, within GFF6587at 29.357 kb on - strand, within GFF6587at 29.357 kb on - strand, within GFF6587at 29.357 kb on - strand, within GFF6587at 29.365 kb on + strand, within GFF6587at 29.365 kb on + strand, within GFF6587at 29.366 kb on - strand, within GFF6587at 29.434 kb on + strand, within GFF6587at 29.434 kb on + strand, within GFF6587at 29.434 kb on + strand, within GFF6587at 29.435 kb on - strand, within GFF6587at 29.435 kb on - strand, within GFF6587at 29.676 kb on + strand, within GFF6587at 29.676 kb on + strand, within GFF6587at 29.677 kb on - strand, within GFF6587at 29.901 kb on + strandat 29.901 kb on + strandat 29.902 kb on - strandat 29.907 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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27,210 + GFF6585 0.27 -0.9
27,519 - GFF6585 0.50 -0.4
27,849 + GFF6585 0.75 -0.0
27,951 - GFF6585 0.83 -0.3
27,967 + GFF6585 0.84 -0.7
27,968 - GFF6585 0.84 +0.4
27,968 - GFF6585 0.84 -0.2
27,996 - GFF6585 0.86 +0.5
28,069 + +1.3
28,641 + GFF6586 0.51 -0.5
28,642 - GFF6586 0.51 -0.4
28,723 + GFF6586 0.60 -0.4
28,723 + GFF6586 0.60 -0.7
28,979 - GFF6586 0.88 -0.2
28,979 - GFF6586 0.88 -0.0
29,086 + -0.9
29,086 + -1.7
29,086 + -0.6
29,086 + -0.4
29,087 - +0.9
29,087 - +0.3
29,087 - +0.3
29,087 - +0.1
29,087 - -0.3
29,116 + +0.4
29,117 - +0.4
29,173 + -1.2
29,174 - -0.5
29,309 - GFF6587 0.25 -0.4
29,309 - GFF6587 0.25 +0.2
29,309 - GFF6587 0.25 +0.3
29,356 + GFF6587 0.31 +0.4
29,356 + GFF6587 0.31 -0.4
29,356 + GFF6587 0.31 -0.1
29,356 + GFF6587 0.31 -0.6
29,356 + GFF6587 0.31 +0.4
29,356 + GFF6587 0.31 +0.1
29,356 + GFF6587 0.31 +0.1
29,357 - GFF6587 0.31 +1.1
29,357 - GFF6587 0.31 -1.8
29,357 - GFF6587 0.31 -0.7
29,365 + GFF6587 0.32 -0.8
29,365 + GFF6587 0.32 -0.2
29,366 - GFF6587 0.32 -1.7
29,434 + GFF6587 0.41 +0.7
29,434 + GFF6587 0.41 +0.2
29,434 + GFF6587 0.41 +0.3
29,435 - GFF6587 0.41 +0.4
29,435 - GFF6587 0.41 -0.6
29,676 + GFF6587 0.72 -1.2
29,676 + GFF6587 0.72 -0.4
29,677 - GFF6587 0.72 -1.8
29,901 + +0.6
29,901 + +0.1
29,902 - -0.8
29,907 + -0.9

Or see this region's nucleotide sequence