Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6541 and GFF6542 are separated by 183 nucleotides GFF6542 and GFF6543 overlap by 4 nucleotides
GFF6541 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 38,459 to 39,262
GFF6541
GFF6542 - Transcriptional regulator, AcrR family, at 39,446 to 40,162
GFF6542
GFF6543 - Transcriptional regulator, LysR family, at 40,159 to 41,133
GFF6543
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 38.688 kb on + strand, within GFF6541 at 39.280 kb on + strand at 39.281 kb on - strand at 39.307 kb on + strand at 39.410 kb on + strand at 39.411 kb on - strand at 39.411 kb on - strand at 39.411 kb on - strand at 39.432 kb on + strand at 39.433 kb on - strand at 39.590 kb on + strand, within GFF6542 at 39.591 kb on - strand, within GFF6542 at 39.654 kb on - strand, within GFF6542 at 39.654 kb on - strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.701 kb on + strand, within GFF6542 at 39.702 kb on - strand, within GFF6542 at 39.702 kb on - strand, within GFF6542 at 39.725 kb on + strand, within GFF6542 at 39.725 kb on + strand, within GFF6542 at 39.726 kb on - strand, within GFF6542 at 39.726 kb on - strand, within GFF6542 at 39.834 kb on - strand, within GFF6542 at 39.983 kb on + strand, within GFF6542 at 39.984 kb on - strand, within GFF6542 at 39.984 kb on - strand, within GFF6542 at 40.160 kb on + strand at 40.448 kb on + strand, within GFF6543 at 40.934 kb on + strand, within GFF6543 at 40.934 kb on + strand, within GFF6543 at 40.935 kb on - strand, within GFF6543 at 40.941 kb on - strand, within GFF6543 at 41.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 38,688 + GFF6541 0.28 +1.4 39,280 + -2.2 39,281 - -0.7 39,307 + -0.6 39,410 + +0.2 39,411 - -0.3 39,411 - -0.3 39,411 - +0.7 39,432 + +0.3 39,433 - -0.1 39,590 + GFF6542 0.20 -0.2 39,591 - GFF6542 0.20 -0.7 39,654 - GFF6542 0.29 +1.0 39,654 - GFF6542 0.29 +0.0 39,656 + GFF6542 0.29 +0.7 39,656 + GFF6542 0.29 -0.5 39,656 + GFF6542 0.29 -2.7 39,656 + GFF6542 0.29 +0.4 39,656 + GFF6542 0.29 +0.9 39,701 + GFF6542 0.36 -0.7 39,702 - GFF6542 0.36 +0.3 39,702 - GFF6542 0.36 -0.9 39,725 + GFF6542 0.39 -0.0 39,725 + GFF6542 0.39 -0.7 39,726 - GFF6542 0.39 -0.3 39,726 - GFF6542 0.39 -1.6 39,834 - GFF6542 0.54 -0.1 39,983 + GFF6542 0.75 -0.0 39,984 - GFF6542 0.75 -1.1 39,984 - GFF6542 0.75 +0.4 40,160 + -0.9 40,448 + GFF6543 0.30 -0.2 40,934 + GFF6543 0.79 -0.2 40,934 + GFF6543 0.79 +0.3 40,935 - GFF6543 0.80 -0.4 40,941 - GFF6543 0.80 -0.3 41,140 - -0.8
Or see this region's nucleotide sequence