Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6468 and GFF6469 overlap by 4 nucleotides GFF6469 and GFF6470 overlap by 4 nucleotides GFF6470 and GFF6471 overlap by 13 nucleotides GFF6471 and GFF6472 are separated by 91 nucleotides
GFF6468 - TRAP-type transport system, periplasmic component, predicted N-acetylneuraminate-binding protein, at 45,262 to 46,230
GFF6468
GFF6469 - TRAP-type transport system, large permease component, predicted N-acetylneuraminate transporter, at 46,227 to 46,721
GFF6469
GFF6470 - TRAP-type C4-dicarboxylate transport system, large permease component, at 46,718 to 47,995
GFF6470
GFF6471 - Transcriptional regulator, AraC family, at 47,983 to 48,771
GFF6471
GFF6472 - membrane efflux protein, at 48,863 to 50,056
GFF6472
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 45.790 kb on + strand, within GFF6468 at 45.791 kb on - strand, within GFF6468 at 45.791 kb on - strand, within GFF6468 at 46.222 kb on + strand at 46.223 kb on - strand at 46.223 kb on - strand at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.405 kb on + strand, within GFF6469 at 46.406 kb on - strand, within GFF6469 at 46.406 kb on - strand, within GFF6469 at 46.406 kb on - strand, within GFF6469 at 46.406 kb on - strand, within GFF6469 at 46.465 kb on - strand, within GFF6469 at 46.563 kb on + strand, within GFF6469 at 46.626 kb on + strand, within GFF6469 at 46.626 kb on + strand, within GFF6469 at 46.680 kb on + strand at 46.681 kb on - strand at 46.681 kb on - strand at 46.847 kb on + strand, within GFF6470 at 46.899 kb on + strand, within GFF6470 at 46.899 kb on + strand, within GFF6470 at 46.899 kb on + strand, within GFF6470 at 46.900 kb on - strand, within GFF6470 at 46.900 kb on - strand, within GFF6470 at 47.115 kb on - strand, within GFF6470 at 47.134 kb on + strand, within GFF6470 at 47.135 kb on - strand, within GFF6470 at 47.135 kb on - strand, within GFF6470 at 47.135 kb on - strand, within GFF6470 at 47.183 kb on + strand, within GFF6470 at 47.187 kb on - strand, within GFF6470 at 47.285 kb on + strand, within GFF6470 at 47.285 kb on + strand, within GFF6470 at 47.285 kb on + strand, within GFF6470 at 47.285 kb on + strand, within GFF6470 at 47.286 kb on - strand, within GFF6470 at 47.286 kb on - strand, within GFF6470 at 47.402 kb on + strand, within GFF6470 at 47.402 kb on + strand, within GFF6470 at 47.403 kb on - strand, within GFF6470 at 47.403 kb on - strand, within GFF6470 at 47.403 kb on - strand, within GFF6470 at 47.450 kb on + strand, within GFF6470 at 47.450 kb on + strand, within GFF6470 at 47.450 kb on + strand, within GFF6470 at 47.451 kb on - strand, within GFF6470 at 47.478 kb on - strand, within GFF6470 at 47.478 kb on - strand, within GFF6470 at 47.504 kb on + strand, within GFF6470 at 47.504 kb on + strand, within GFF6470 at 47.504 kb on + strand, within GFF6470 at 47.504 kb on + strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.505 kb on - strand, within GFF6470 at 47.945 kb on + strand at 47.945 kb on + strand at 47.945 kb on + strand at 47.945 kb on + strand at 47.945 kb on + strand at 47.945 kb on + strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.946 kb on - strand at 47.984 kb on + strand at 47.985 kb on - strand at 47.993 kb on + strand at 47.993 kb on + strand at 47.993 kb on + strand at 47.993 kb on + strand at 47.993 kb on + strand at 47.994 kb on - strand at 47.994 kb on - strand at 47.994 kb on - strand at 48.009 kb on - strand at 48.009 kb on - strand at 48.071 kb on + strand, within GFF6471 at 48.071 kb on + strand, within GFF6471 at 48.399 kb on - strand, within GFF6471 at 48.399 kb on - strand, within GFF6471 at 48.399 kb on - strand, within GFF6471 at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.823 kb on + strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.824 kb on - strand at 48.917 kb on + strand at 48.917 kb on + strand at 48.968 kb on + strand at 48.968 kb on + strand at 48.969 kb on - strand at 48.974 kb on + strand at 48.974 kb on + strand at 48.974 kb on + strand at 48.974 kb on + strand at 48.974 kb on + strand at 48.975 kb on - strand at 48.975 kb on - strand at 48.975 kb on - strand at 48.975 kb on - strand at 48.975 kb on - strand at 48.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 45,790 + GFF6468 0.54 -0.0 45,791 - GFF6468 0.55 +0.7 45,791 - GFF6468 0.55 -0.5 46,222 + +0.3 46,223 - +0.1 46,223 - -0.6 46,405 + GFF6469 0.36 +0.2 46,405 + GFF6469 0.36 -0.2 46,405 + GFF6469 0.36 -0.2 46,405 + GFF6469 0.36 +0.8 46,405 + GFF6469 0.36 -0.0 46,405 + GFF6469 0.36 +1.5 46,405 + GFF6469 0.36 -3.1 46,405 + GFF6469 0.36 +0.4 46,406 - GFF6469 0.36 +0.1 46,406 - GFF6469 0.36 -1.2 46,406 - GFF6469 0.36 +0.2 46,406 - GFF6469 0.36 -0.5 46,465 - GFF6469 0.48 -1.4 46,563 + GFF6469 0.68 -0.6 46,626 + GFF6469 0.81 -0.5 46,626 + GFF6469 0.81 -0.0 46,680 + +1.4 46,681 - -0.9 46,681 - +0.2 46,847 + GFF6470 0.10 +0.7 46,899 + GFF6470 0.14 -0.4 46,899 + GFF6470 0.14 -0.1 46,899 + GFF6470 0.14 -0.9 46,900 - GFF6470 0.14 -0.4 46,900 - GFF6470 0.14 +0.4 47,115 - GFF6470 0.31 -0.8 47,134 + GFF6470 0.33 -0.7 47,135 - GFF6470 0.33 +1.4 47,135 - GFF6470 0.33 +0.3 47,135 - GFF6470 0.33 -0.0 47,183 + GFF6470 0.36 -1.8 47,187 - GFF6470 0.37 +1.0 47,285 + GFF6470 0.44 +1.0 47,285 + GFF6470 0.44 -0.3 47,285 + GFF6470 0.44 +0.2 47,285 + GFF6470 0.44 +1.1 47,286 - GFF6470 0.44 -0.2 47,286 - GFF6470 0.44 -1.6 47,402 + GFF6470 0.54 +0.4 47,402 + GFF6470 0.54 -0.5 47,403 - GFF6470 0.54 -1.6 47,403 - GFF6470 0.54 -0.0 47,403 - GFF6470 0.54 +0.8 47,450 + GFF6470 0.57 -0.9 47,450 + GFF6470 0.57 +1.2 47,450 + GFF6470 0.57 +0.2 47,451 - GFF6470 0.57 +1.3 47,478 - GFF6470 0.59 -0.9 47,478 - GFF6470 0.59 +0.1 47,504 + GFF6470 0.62 -0.9 47,504 + GFF6470 0.62 -0.8 47,504 + GFF6470 0.62 +2.4 47,504 + GFF6470 0.62 +0.7 47,505 - GFF6470 0.62 -0.2 47,505 - GFF6470 0.62 +1.0 47,505 - GFF6470 0.62 +0.4 47,505 - GFF6470 0.62 -0.3 47,505 - GFF6470 0.62 -1.3 47,505 - GFF6470 0.62 -0.1 47,945 + -0.5 47,945 + -0.1 47,945 + -0.3 47,945 + +0.8 47,945 + -0.3 47,945 + +0.5 47,946 - +0.0 47,946 - -0.4 47,946 - +1.1 47,946 - -0.3 47,946 - +0.0 47,946 - -0.8 47,946 - -0.6 47,946 - -1.4 47,946 - +1.7 47,984 + +0.4 47,985 - -1.7 47,993 + +0.9 47,993 + -1.2 47,993 + -0.6 47,993 + -0.9 47,993 + +0.2 47,994 - -2.0 47,994 - -2.3 47,994 - -0.6 48,009 - -0.8 48,009 - +0.1 48,071 + GFF6471 0.11 +1.4 48,071 + GFF6471 0.11 -0.0 48,399 - GFF6471 0.53 +0.2 48,399 - GFF6471 0.53 -0.4 48,399 - GFF6471 0.53 -0.7 48,823 + -0.5 48,823 + -0.0 48,823 + -0.7 48,823 + +0.3 48,823 + +0.5 48,823 + -0.5 48,823 + -0.3 48,823 + -0.3 48,824 - -0.9 48,824 - -0.0 48,824 - +0.5 48,824 - +0.1 48,824 - -1.4 48,824 - -0.6 48,824 - +0.6 48,824 - +0.4 48,824 - -0.4 48,824 - -0.3 48,917 + +0.4 48,917 + -1.8 48,968 + -0.3 48,968 + -0.2 48,969 - -0.6 48,974 + -0.2 48,974 + -0.7 48,974 + -0.1 48,974 + -0.2 48,974 + -1.2 48,975 - -1.6 48,975 - -0.3 48,975 - -0.8 48,975 - +0.2 48,975 - -0.2 48,975 - -0.7
Or see this region's nucleotide sequence