Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6413 and GFF6414 are separated by 120 nucleotides GFF6414 and GFF6415 are separated by 54 nucleotides GFF6415 and GFF6416 are separated by 37 nucleotides GFF6416 and GFF6417 are separated by 16 nucleotides
GFF6413 - Histidine ABC transporter, substrate-binding protein, at 87,654 to 88,625
GFF6413
GFF6414 - no description, at 88,746 to 88,982
GFF6414
GFF6415 - ATP-dependent DNA helicase RecQ, at 89,037 to 90,812
GFF6415
GFF6416 - hypothetical protein, at 90,850 to 91,716
GFF6416
GFF6417 - BUG/TctC family periplasmic protein, at 91,733 to 92,725
GFF6417
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.196 kb on - strand, within GFF6413 at 88.196 kb on - strand, within GFF6413 at 88.291 kb on + strand, within GFF6413 at 88.292 kb on - strand, within GFF6413 at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.629 kb on + strand at 88.629 kb on + strand at 88.629 kb on + strand at 88.723 kb on + strand at 88.723 kb on + strand at 88.724 kb on - strand at 88.724 kb on - strand at 88.726 kb on - strand at 88.726 kb on - strand at 88.727 kb on + strand at 88.727 kb on + strand at 88.728 kb on - strand at 88.728 kb on - strand at 88.791 kb on - strand, within GFF6414 at 88.976 kb on - strand at 88.996 kb on - strand at 89.097 kb on + strand at 89.097 kb on + strand at 89.097 kb on + strand at 89.097 kb on + strand at 89.097 kb on + strand at 89.097 kb on + strand at 89.098 kb on - strand at 89.298 kb on + strand, within GFF6415 at 89.298 kb on + strand, within GFF6415 at 89.299 kb on - strand, within GFF6415 at 89.437 kb on - strand, within GFF6415 at 89.466 kb on + strand, within GFF6415 at 89.466 kb on + strand, within GFF6415 at 89.467 kb on - strand, within GFF6415 at 89.467 kb on - strand, within GFF6415 at 89.467 kb on - strand, within GFF6415 at 89.595 kb on + strand, within GFF6415 at 89.595 kb on + strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.596 kb on - strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.679 kb on + strand, within GFF6415 at 89.680 kb on - strand, within GFF6415 at 89.680 kb on - strand, within GFF6415 at 89.680 kb on - strand, within GFF6415 at 89.680 kb on - strand, within GFF6415 at 89.752 kb on - strand, within GFF6415 at 89.752 kb on - strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.913 kb on + strand, within GFF6415 at 89.914 kb on - strand, within GFF6415 at 89.914 kb on - strand, within GFF6415 at 89.914 kb on - strand, within GFF6415 at 89.914 kb on - strand, within GFF6415 at 89.914 kb on - strand, within GFF6415 at 89.916 kb on + strand, within GFF6415 at 89.917 kb on - strand, within GFF6415 at 89.917 kb on - strand, within GFF6415 at 89.973 kb on + strand, within GFF6415 at 89.973 kb on + strand, within GFF6415 at 89.973 kb on + strand, within GFF6415 at 89.973 kb on + strand, within GFF6415 at 89.974 kb on - strand, within GFF6415 at 89.974 kb on - strand, within GFF6415 at 90.120 kb on + strand, within GFF6415 at 90.120 kb on + strand, within GFF6415 at 90.120 kb on + strand, within GFF6415 at 90.120 kb on + strand, within GFF6415 at 90.121 kb on - strand, within GFF6415 at 90.121 kb on - strand, within GFF6415 at 90.121 kb on - strand, within GFF6415 at 90.121 kb on - strand, within GFF6415 at 90.121 kb on - strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.225 kb on + strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.226 kb on - strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.335 kb on + strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.336 kb on - strand, within GFF6415 at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.667 kb on - strand at 90.774 kb on + strand at 90.775 kb on - strand at 91.054 kb on + strand, within GFF6416 at 91.054 kb on + strand, within GFF6416 at 91.055 kb on - strand, within GFF6416 at 91.055 kb on - strand, within GFF6416 at 91.141 kb on + strand, within GFF6416 at 91.141 kb on + strand, within GFF6416 at 91.141 kb on + strand, within GFF6416 at 91.141 kb on + strand, within GFF6416 at 91.141 kb on + strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.142 kb on - strand, within GFF6416 at 91.603 kb on + strand, within GFF6416 at 91.604 kb on - strand, within GFF6416
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 88,060 + GFF6413 0.42 +0.2 88,060 + GFF6413 0.42 +0.1 88,060 + GFF6413 0.42 +1.2 88,060 + GFF6413 0.42 -0.1 88,061 - GFF6413 0.42 -2.2 88,061 - GFF6413 0.42 +0.2 88,061 - GFF6413 0.42 -0.1 88,061 - GFF6413 0.42 +0.5 88,061 - GFF6413 0.42 +0.0 88,069 + GFF6413 0.43 -0.8 88,069 + GFF6413 0.43 -0.7 88,069 + GFF6413 0.43 -1.0 88,069 + GFF6413 0.43 -1.8 88,069 + GFF6413 0.43 -0.7 88,069 + GFF6413 0.43 +0.2 88,069 + GFF6413 0.43 +0.8 88,069 + GFF6413 0.43 -0.6 88,069 + GFF6413 0.43 -0.9 88,070 - GFF6413 0.43 -0.8 88,070 - GFF6413 0.43 -1.2 88,070 - GFF6413 0.43 -0.2 88,070 - GFF6413 0.43 +0.2 88,070 - GFF6413 0.43 +0.5 88,070 - GFF6413 0.43 -0.1 88,070 - GFF6413 0.43 +0.1 88,070 - GFF6413 0.43 -0.0 88,070 - GFF6413 0.43 -0.6 88,070 - GFF6413 0.43 +0.4 88,070 - GFF6413 0.43 +0.0 88,070 - GFF6413 0.43 +1.3 88,070 - GFF6413 0.43 +0.6 88,070 - GFF6413 0.43 -0.5 88,070 - GFF6413 0.43 -1.9 88,070 - GFF6413 0.43 +0.2 88,195 + GFF6413 0.56 -1.8 88,195 + GFF6413 0.56 +0.1 88,195 + GFF6413 0.56 +0.6 88,196 - GFF6413 0.56 -0.1 88,196 - GFF6413 0.56 -0.3 88,291 + GFF6413 0.66 -0.1 88,292 - GFF6413 0.66 +0.3 88,608 + -0.4 88,608 + +0.7 88,608 + -0.1 88,608 + -1.1 88,608 + +0.5 88,608 + -0.2 88,608 + +0.5 88,608 + -0.3 88,609 - +0.4 88,609 - +0.2 88,609 - +0.2 88,609 - +1.1 88,609 - -1.2 88,609 - +0.0 88,629 + +0.5 88,629 + +0.8 88,629 + -0.1 88,723 + +0.5 88,723 + -0.2 88,724 - -1.6 88,724 - -0.7 88,726 - -0.1 88,726 - -0.5 88,727 + -0.3 88,727 + -0.5 88,728 - +0.0 88,728 - -0.6 88,791 - GFF6414 0.19 -0.5 88,976 - +1.5 88,996 - -0.1 89,097 + +0.4 89,097 + +0.8 89,097 + -0.3 89,097 + -0.8 89,097 + +0.8 89,097 + +1.0 89,098 - -2.3 89,298 + GFF6415 0.15 -0.5 89,298 + GFF6415 0.15 +0.2 89,299 - GFF6415 0.15 +0.3 89,437 - GFF6415 0.23 +0.2 89,466 + GFF6415 0.24 -0.1 89,466 + GFF6415 0.24 -0.0 89,467 - GFF6415 0.24 -0.1 89,467 - GFF6415 0.24 +1.6 89,467 - GFF6415 0.24 -1.2 89,595 + GFF6415 0.31 -1.5 89,595 + GFF6415 0.31 -0.9 89,596 - GFF6415 0.31 -0.7 89,596 - GFF6415 0.31 +0.2 89,596 - GFF6415 0.31 +0.1 89,596 - GFF6415 0.31 -0.8 89,596 - GFF6415 0.31 -0.3 89,596 - GFF6415 0.31 -1.0 89,596 - GFF6415 0.31 +2.8 89,679 + GFF6415 0.36 -0.5 89,679 + GFF6415 0.36 +0.8 89,679 + GFF6415 0.36 -0.0 89,679 + GFF6415 0.36 -0.6 89,679 + GFF6415 0.36 +0.4 89,679 + GFF6415 0.36 -0.3 89,679 + GFF6415 0.36 -1.1 89,679 + GFF6415 0.36 -0.2 89,679 + GFF6415 0.36 +0.2 89,679 + GFF6415 0.36 +0.3 89,680 - GFF6415 0.36 -0.4 89,680 - GFF6415 0.36 -0.7 89,680 - GFF6415 0.36 -0.6 89,680 - GFF6415 0.36 -0.5 89,752 - GFF6415 0.40 +0.4 89,752 - GFF6415 0.40 +0.2 89,913 + GFF6415 0.49 +0.4 89,913 + GFF6415 0.49 +0.1 89,913 + GFF6415 0.49 -1.0 89,913 + GFF6415 0.49 +1.1 89,913 + GFF6415 0.49 +0.2 89,913 + GFF6415 0.49 -0.5 89,913 + GFF6415 0.49 -1.0 89,913 + GFF6415 0.49 +1.4 89,914 - GFF6415 0.49 -1.7 89,914 - GFF6415 0.49 -0.1 89,914 - GFF6415 0.49 -0.5 89,914 - GFF6415 0.49 -1.3 89,914 - GFF6415 0.49 +0.4 89,916 + GFF6415 0.49 +0.4 89,917 - GFF6415 0.50 +0.2 89,917 - GFF6415 0.50 -0.2 89,973 + GFF6415 0.53 +0.8 89,973 + GFF6415 0.53 -0.4 89,973 + GFF6415 0.53 -0.9 89,973 + GFF6415 0.53 -0.5 89,974 - GFF6415 0.53 -1.3 89,974 - GFF6415 0.53 +0.2 90,120 + GFF6415 0.61 -0.5 90,120 + GFF6415 0.61 -0.5 90,120 + GFF6415 0.61 +0.3 90,120 + GFF6415 0.61 +0.4 90,121 - GFF6415 0.61 -0.3 90,121 - GFF6415 0.61 +0.1 90,121 - GFF6415 0.61 +0.2 90,121 - GFF6415 0.61 -0.1 90,121 - GFF6415 0.61 -0.7 90,225 + GFF6415 0.67 -0.8 90,225 + GFF6415 0.67 +0.0 90,225 + GFF6415 0.67 +0.6 90,225 + GFF6415 0.67 -0.8 90,225 + GFF6415 0.67 -1.4 90,225 + GFF6415 0.67 +0.2 90,225 + GFF6415 0.67 -0.0 90,226 - GFF6415 0.67 -1.0 90,226 - GFF6415 0.67 -0.4 90,226 - GFF6415 0.67 +0.2 90,226 - GFF6415 0.67 +1.1 90,226 - GFF6415 0.67 -0.6 90,226 - GFF6415 0.67 -1.5 90,226 - GFF6415 0.67 -2.4 90,226 - GFF6415 0.67 -0.2 90,335 + GFF6415 0.73 -2.0 90,335 + GFF6415 0.73 +0.1 90,335 + GFF6415 0.73 +0.2 90,335 + GFF6415 0.73 +0.6 90,335 + GFF6415 0.73 +1.6 90,335 + GFF6415 0.73 -0.2 90,335 + GFF6415 0.73 -0.6 90,335 + GFF6415 0.73 +0.5 90,335 + GFF6415 0.73 +1.8 90,336 - GFF6415 0.73 -0.5 90,336 - GFF6415 0.73 -1.2 90,336 - GFF6415 0.73 -0.5 90,336 - GFF6415 0.73 +0.7 90,336 - GFF6415 0.73 -0.5 90,336 - GFF6415 0.73 -0.7 90,336 - GFF6415 0.73 +0.7 90,666 + -0.2 90,666 + +0.2 90,666 + -1.0 90,666 + +0.1 90,666 + -1.0 90,666 + +0.4 90,667 - +0.2 90,667 - -0.2 90,667 - -0.8 90,667 - -0.1 90,667 - +0.1 90,667 - +0.5 90,667 - -0.3 90,667 - +0.1 90,667 - -0.9 90,667 - -1.1 90,774 + -0.7 90,775 - -1.4 91,054 + GFF6416 0.24 -0.2 91,054 + GFF6416 0.24 +1.1 91,055 - GFF6416 0.24 -0.7 91,055 - GFF6416 0.24 -1.0 91,141 + GFF6416 0.34 -0.6 91,141 + GFF6416 0.34 +0.5 91,141 + GFF6416 0.34 -0.5 91,141 + GFF6416 0.34 -0.3 91,141 + GFF6416 0.34 -0.6 91,142 - GFF6416 0.34 +0.1 91,142 - GFF6416 0.34 +0.8 91,142 - GFF6416 0.34 -0.2 91,142 - GFF6416 0.34 +1.2 91,142 - GFF6416 0.34 -0.8 91,142 - GFF6416 0.34 -0.2 91,603 + GFF6416 0.87 -0.6 91,604 - GFF6416 0.87 +0.2
Or see this region's nucleotide sequence