Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6208 and GFF6209 are separated by 192 nucleotides GFF6209 and GFF6210 overlap by 4 nucleotides GFF6210 and GFF6211 are separated by 25 nucleotides
GFF6208 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 115,313 to 116,806
GFF6208
GFF6209 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 116,999 to 117,742
GFF6209
GFF6210 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 117,739 to 118,527
GFF6210
GFF6211 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 118,553 to 119,317
GFF6211
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-1
0
1
2
3 at 116.025 kb on + strand, within GFF6208 at 116.025 kb on + strand, within GFF6208 at 116.025 kb on + strand, within GFF6208 at 116.025 kb on + strand, within GFF6208 at 116.026 kb on - strand, within GFF6208 at 116.026 kb on - strand, within GFF6208 at 116.166 kb on + strand, within GFF6208 at 116.166 kb on + strand, within GFF6208 at 116.373 kb on + strand, within GFF6208 at 116.374 kb on - strand, within GFF6208 at 116.374 kb on - strand, within GFF6208 at 116.607 kb on + strand, within GFF6208 at 116.607 kb on + strand, within GFF6208 at 116.608 kb on - strand, within GFF6208 at 116.655 kb on + strand, within GFF6208 at 116.655 kb on + strand, within GFF6208 at 116.655 kb on + strand, within GFF6208 at 116.655 kb on + strand, within GFF6208 at 116.656 kb on - strand, within GFF6208 at 116.656 kb on - strand, within GFF6208 at 116.656 kb on - strand, within GFF6208 at 116.656 kb on - strand, within GFF6208 at 116.716 kb on - strand at 116.932 kb on + strand at 116.932 kb on + strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.933 kb on - strand at 116.934 kb on + strand at 116.934 kb on + strand at 116.935 kb on - strand at 116.940 kb on + strand at 116.941 kb on - strand at 116.941 kb on - strand at 117.241 kb on + strand, within GFF6209 at 117.241 kb on + strand, within GFF6209 at 117.242 kb on - strand, within GFF6209 at 117.242 kb on - strand, within GFF6209 at 117.368 kb on + strand, within GFF6209 at 117.818 kb on + strand, within GFF6210 at 117.818 kb on + strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.819 kb on - strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.883 kb on + strand, within GFF6210 at 117.884 kb on - strand, within GFF6210 at 117.884 kb on - strand, within GFF6210 at 118.013 kb on + strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.014 kb on - strand, within GFF6210 at 118.100 kb on + strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.101 kb on - strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.236 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.238 kb on + strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.239 kb on - strand, within GFF6210 at 118.564 kb on + strand at 118.564 kb on + strand at 118.564 kb on + strand at 118.564 kb on + strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.565 kb on - strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.581 kb on + strand at 118.582 kb on - strand at 118.582 kb on - strand at 118.582 kb on - strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.602 kb on + strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.603 kb on - strand at 118.641 kb on + strand, within GFF6211
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 116,025 + GFF6208 0.48 -1.6 116,025 + GFF6208 0.48 +2.1 116,025 + GFF6208 0.48 -0.6 116,025 + GFF6208 0.48 -0.1 116,026 - GFF6208 0.48 +0.1 116,026 - GFF6208 0.48 -0.5 116,166 + GFF6208 0.57 -0.0 116,166 + GFF6208 0.57 +0.5 116,373 + GFF6208 0.71 +1.4 116,374 - GFF6208 0.71 -0.3 116,374 - GFF6208 0.71 +0.1 116,607 + GFF6208 0.87 +3.0 116,607 + GFF6208 0.87 -0.3 116,608 - GFF6208 0.87 +0.1 116,655 + GFF6208 0.90 -0.2 116,655 + GFF6208 0.90 -0.6 116,655 + GFF6208 0.90 -0.8 116,655 + GFF6208 0.90 +0.5 116,656 - GFF6208 0.90 +0.9 116,656 - GFF6208 0.90 -0.4 116,656 - GFF6208 0.90 -0.3 116,656 - GFF6208 0.90 +0.7 116,716 - -0.0 116,932 + +0.3 116,932 + +0.9 116,933 - +0.8 116,933 - -0.1 116,933 - -0.8 116,933 - -0.4 116,934 + +0.3 116,934 + +0.2 116,935 - -0.9 116,940 + +0.1 116,941 - -0.8 116,941 - -0.4 117,241 + GFF6209 0.33 -1.2 117,241 + GFF6209 0.33 +0.2 117,242 - GFF6209 0.33 -1.1 117,242 - GFF6209 0.33 -0.6 117,368 + GFF6209 0.50 -0.8 117,818 + GFF6210 0.10 +0.9 117,818 + GFF6210 0.10 -0.9 117,819 - GFF6210 0.10 -0.2 117,819 - GFF6210 0.10 -0.4 117,819 - GFF6210 0.10 -1.6 117,819 - GFF6210 0.10 -0.0 117,819 - GFF6210 0.10 -1.4 117,883 + GFF6210 0.18 -0.6 117,883 + GFF6210 0.18 +0.6 117,883 + GFF6210 0.18 -1.1 117,883 + GFF6210 0.18 +1.4 117,883 + GFF6210 0.18 -0.5 117,884 - GFF6210 0.18 -0.2 117,884 - GFF6210 0.18 -0.2 118,013 + GFF6210 0.35 +0.3 118,014 - GFF6210 0.35 -0.5 118,014 - GFF6210 0.35 -0.2 118,014 - GFF6210 0.35 -0.6 118,100 + GFF6210 0.46 +0.1 118,101 - GFF6210 0.46 -0.1 118,101 - GFF6210 0.46 +1.7 118,101 - GFF6210 0.46 -0.7 118,101 - GFF6210 0.46 -0.0 118,236 + GFF6210 0.63 +0.2 118,236 + GFF6210 0.63 -1.3 118,236 + GFF6210 0.63 -0.1 118,236 + GFF6210 0.63 -0.0 118,238 + GFF6210 0.63 +1.1 118,238 + GFF6210 0.63 +0.0 118,238 + GFF6210 0.63 -0.4 118,238 + GFF6210 0.63 +0.7 118,238 + GFF6210 0.63 +1.4 118,239 - GFF6210 0.63 +1.0 118,239 - GFF6210 0.63 +0.3 118,239 - GFF6210 0.63 +0.7 118,239 - GFF6210 0.63 +0.1 118,239 - GFF6210 0.63 +2.0 118,239 - GFF6210 0.63 -1.6 118,239 - GFF6210 0.63 -0.5 118,239 - GFF6210 0.63 -0.1 118,564 + -0.6 118,564 + -1.2 118,564 + +1.2 118,564 + +0.8 118,565 - -0.7 118,565 - +0.5 118,565 - -0.2 118,565 - -0.8 118,565 - -0.3 118,581 + +0.5 118,581 + -1.2 118,581 + -0.2 118,581 + -0.5 118,582 - -0.8 118,582 - +0.6 118,582 - +0.2 118,602 + +1.1 118,602 + -1.0 118,602 + -0.1 118,602 + -1.2 118,603 - +1.6 118,603 - -0.4 118,603 - -1.2 118,603 - -0.8 118,603 - -1.0 118,603 - +0.3 118,641 + GFF6211 0.12 -0.8
Or see this region's nucleotide sequence