Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6187 and GFF6188 are separated by 129 nucleotides GFF6188 and GFF6189 overlap by 4 nucleotides GFF6189 and GFF6190 are separated by 451 nucleotides
GFF6187 - Outer membrane protein A precursor, at 93,167 to 93,817
GFF6187
GFF6188 - 3-demethylubiquinol 3-O-methyltransferase (EC 2.1.1.64) @ 2-polyprenyl-6-hydroxyphenyl methylase (EC 2.1.1.222), at 93,947 to 94,666
GFF6188
GFF6189 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 94,663 to 95,334
GFF6189
GFF6190 - 3-hydroxyisobutyrate dehydrogenase family protein, at 95,786 to 96,676
GFF6190
Position (kb) 94
95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 93.815 kb on + strand at 93.815 kb on + strand at 93.815 kb on + strand at 93.815 kb on + strand at 93.815 kb on + strand at 93.816 kb on - strand at 93.816 kb on - strand at 93.816 kb on - strand at 93.866 kb on + strand at 93.880 kb on + strand at 93.881 kb on - strand at 93.881 kb on - strand at 93.881 kb on - strand at 93.917 kb on - strand at 93.937 kb on + strand at 93.937 kb on + strand at 93.937 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.946 kb on + strand at 93.947 kb on - strand at 93.947 kb on - strand at 93.947 kb on - strand at 93.947 kb on - strand at 93.947 kb on - strand at 94.783 kb on + strand, within GFF6189 at 94.783 kb on + strand, within GFF6189 at 94.798 kb on + strand, within GFF6189 at 94.798 kb on + strand, within GFF6189 at 94.799 kb on - strand, within GFF6189 at 94.799 kb on - strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.906 kb on + strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.907 kb on - strand, within GFF6189 at 94.961 kb on + strand, within GFF6189 at 94.961 kb on + strand, within GFF6189 at 94.961 kb on + strand, within GFF6189 at 94.962 kb on - strand, within GFF6189 at 94.962 kb on - strand, within GFF6189 at 94.962 kb on - strand, within GFF6189 at 94.962 kb on - strand, within GFF6189 at 94.962 kb on - strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.167 kb on + strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.168 kb on - strand, within GFF6189 at 95.220 kb on + strand, within GFF6189 at 95.220 kb on + strand, within GFF6189 at 95.220 kb on + strand, within GFF6189 at 95.220 kb on + strand, within GFF6189 at 95.221 kb on - strand, within GFF6189 at 95.221 kb on - strand, within GFF6189 at 95.236 kb on + strand, within GFF6189 at 95.236 kb on + strand, within GFF6189 at 95.236 kb on + strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.237 kb on - strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.242 kb on + strand, within GFF6189 at 95.243 kb on - strand, within GFF6189 at 95.243 kb on - strand, within GFF6189 at 95.291 kb on + strand at 95.291 kb on + strand at 95.291 kb on + strand at 95.291 kb on + strand at 95.291 kb on + strand at 95.292 kb on - strand at 95.292 kb on - strand at 95.292 kb on - strand at 95.318 kb on + strand at 95.318 kb on + strand at 95.412 kb on + strand at 95.412 kb on + strand at 95.413 kb on - strand at 95.432 kb on + strand at 95.432 kb on + strand at 95.433 kb on - strand at 95.433 kb on - strand at 95.433 kb on - strand at 95.467 kb on + strand at 95.467 kb on + strand at 95.468 kb on - strand at 95.468 kb on - strand at 95.550 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.554 kb on + strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.555 kb on - strand at 95.579 kb on - strand at 95.601 kb on + strand at 95.601 kb on + strand at 95.601 kb on + strand at 95.601 kb on + strand at 95.621 kb on + strand at 95.655 kb on + strand at 95.655 kb on + strand at 95.655 kb on + strand at 95.656 kb on - strand at 95.668 kb on + strand at 95.668 kb on + strand at 95.668 kb on + strand at 95.668 kb on + strand at 95.668 kb on + strand at 95.669 kb on - strand at 95.676 kb on + strand at 95.676 kb on + strand at 95.676 kb on + strand at 95.676 kb on + strand at 95.676 kb on + strand at 95.676 kb on + strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.677 kb on - strand at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.916 kb on + strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.917 kb on - strand, within GFF6190 at 95.919 kb on + strand, within GFF6190 at 95.919 kb on + strand, within GFF6190 at 95.920 kb on - strand, within GFF6190 at 95.920 kb on - strand, within GFF6190 at 95.920 kb on - strand, within GFF6190
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 93,815 + +0.7 93,815 + -0.2 93,815 + +0.8 93,815 + -0.2 93,815 + -0.5 93,816 - -0.5 93,816 - -0.5 93,816 - +0.1 93,866 + -0.5 93,880 + -0.5 93,881 - +0.1 93,881 - +1.0 93,881 - -1.3 93,917 - -0.1 93,937 + -0.4 93,937 + -0.5 93,937 + -0.3 93,946 + +0.1 93,946 + -0.1 93,946 + -0.3 93,946 + -0.1 93,946 + -0.3 93,946 + +0.2 93,946 + +0.9 93,946 + -1.8 93,946 + -1.7 93,947 - -0.6 93,947 - +0.5 93,947 - +1.2 93,947 - -1.9 93,947 - -0.2 94,783 + GFF6189 0.18 -0.6 94,783 + GFF6189 0.18 -0.2 94,798 + GFF6189 0.20 -1.7 94,798 + GFF6189 0.20 -0.5 94,799 - GFF6189 0.20 -0.4 94,799 - GFF6189 0.20 -0.3 94,906 + GFF6189 0.36 -0.8 94,906 + GFF6189 0.36 -0.5 94,906 + GFF6189 0.36 +1.0 94,906 + GFF6189 0.36 +0.2 94,906 + GFF6189 0.36 -0.1 94,906 + GFF6189 0.36 +0.6 94,906 + GFF6189 0.36 -0.9 94,906 + GFF6189 0.36 -0.6 94,906 + GFF6189 0.36 -0.3 94,906 + GFF6189 0.36 +0.8 94,906 + GFF6189 0.36 -0.1 94,906 + GFF6189 0.36 -0.2 94,906 + GFF6189 0.36 -0.0 94,906 + GFF6189 0.36 -0.3 94,906 + GFF6189 0.36 -0.4 94,907 - GFF6189 0.36 -0.0 94,907 - GFF6189 0.36 +0.8 94,907 - GFF6189 0.36 -0.4 94,907 - GFF6189 0.36 +0.1 94,907 - GFF6189 0.36 -0.9 94,907 - GFF6189 0.36 -0.4 94,907 - GFF6189 0.36 -1.3 94,907 - GFF6189 0.36 -0.6 94,907 - GFF6189 0.36 +0.6 94,907 - GFF6189 0.36 +0.5 94,907 - GFF6189 0.36 -0.5 94,961 + GFF6189 0.44 +0.1 94,961 + GFF6189 0.44 -0.2 94,961 + GFF6189 0.44 +0.4 94,962 - GFF6189 0.44 +0.3 94,962 - GFF6189 0.44 +1.9 94,962 - GFF6189 0.44 -0.5 94,962 - GFF6189 0.44 -1.2 94,962 - GFF6189 0.44 +0.4 95,167 + GFF6189 0.75 -0.4 95,167 + GFF6189 0.75 +0.8 95,167 + GFF6189 0.75 -0.7 95,167 + GFF6189 0.75 +0.4 95,167 + GFF6189 0.75 -0.9 95,167 + GFF6189 0.75 +0.4 95,167 + GFF6189 0.75 -0.7 95,167 + GFF6189 0.75 +0.9 95,167 + GFF6189 0.75 -0.2 95,167 + GFF6189 0.75 -0.2 95,167 + GFF6189 0.75 +0.2 95,167 + GFF6189 0.75 +0.1 95,167 + GFF6189 0.75 -0.7 95,167 + GFF6189 0.75 -0.9 95,167 + GFF6189 0.75 +0.7 95,167 + GFF6189 0.75 -0.2 95,167 + GFF6189 0.75 +1.1 95,167 + GFF6189 0.75 -0.2 95,167 + GFF6189 0.75 -1.6 95,167 + GFF6189 0.75 -0.2 95,167 + GFF6189 0.75 +1.6 95,167 + GFF6189 0.75 -1.1 95,168 - GFF6189 0.75 -0.9 95,168 - GFF6189 0.75 +1.4 95,168 - GFF6189 0.75 -0.6 95,168 - GFF6189 0.75 -0.6 95,168 - GFF6189 0.75 +0.5 95,168 - GFF6189 0.75 -0.2 95,168 - GFF6189 0.75 -0.1 95,168 - GFF6189 0.75 +0.3 95,168 - GFF6189 0.75 -0.5 95,168 - GFF6189 0.75 -0.8 95,168 - GFF6189 0.75 -0.4 95,168 - GFF6189 0.75 -0.2 95,168 - GFF6189 0.75 -0.5 95,168 - GFF6189 0.75 -0.6 95,168 - GFF6189 0.75 -1.1 95,168 - GFF6189 0.75 -0.4 95,168 - GFF6189 0.75 -0.2 95,220 + GFF6189 0.83 -1.0 95,220 + GFF6189 0.83 -1.9 95,220 + GFF6189 0.83 -0.4 95,220 + GFF6189 0.83 -1.5 95,221 - GFF6189 0.83 +0.1 95,221 - GFF6189 0.83 -0.1 95,236 + GFF6189 0.85 -0.4 95,236 + GFF6189 0.85 -0.2 95,236 + GFF6189 0.85 +0.1 95,237 - GFF6189 0.85 -0.2 95,237 - GFF6189 0.85 +0.7 95,237 - GFF6189 0.85 +2.1 95,237 - GFF6189 0.85 -1.6 95,237 - GFF6189 0.85 -0.5 95,237 - GFF6189 0.85 -0.9 95,237 - GFF6189 0.85 -2.0 95,242 + GFF6189 0.86 -0.0 95,242 + GFF6189 0.86 +2.4 95,242 + GFF6189 0.86 +0.3 95,242 + GFF6189 0.86 -1.2 95,242 + GFF6189 0.86 -1.2 95,242 + GFF6189 0.86 -0.9 95,243 - GFF6189 0.86 -0.6 95,243 - GFF6189 0.86 -0.5 95,291 + -0.7 95,291 + +1.8 95,291 + -1.2 95,291 + -0.9 95,291 + -0.2 95,292 - -3.4 95,292 - +0.1 95,292 - +0.1 95,318 + +0.2 95,318 + -0.5 95,412 + -0.5 95,412 + +0.2 95,413 - +0.4 95,432 + +0.9 95,432 + -1.2 95,433 - +0.3 95,433 - -0.3 95,433 - +0.5 95,467 + -1.4 95,467 + -0.3 95,468 - -0.2 95,468 - +0.2 95,550 + +1.0 95,554 + -0.5 95,554 + -0.6 95,554 + +1.0 95,554 + -2.4 95,554 + +0.4 95,554 + -0.9 95,554 + -0.6 95,554 + +0.2 95,554 + +0.7 95,554 + +0.1 95,554 + -0.2 95,554 + +0.6 95,554 + -0.2 95,554 + +0.6 95,554 + +0.1 95,554 + -1.6 95,554 + +0.8 95,554 + +1.7 95,554 + +1.4 95,554 + +1.2 95,554 + -0.2 95,555 - -0.8 95,555 - -0.3 95,555 - -0.8 95,555 - +1.4 95,555 - +0.5 95,555 - -0.1 95,555 - +0.6 95,555 - +0.2 95,555 - +0.1 95,555 - -0.5 95,579 - +0.8 95,601 + +1.1 95,601 + -2.9 95,601 + -0.1 95,601 + -0.4 95,621 + -0.6 95,655 + -0.8 95,655 + +0.7 95,655 + +0.3 95,656 - -0.6 95,668 + +0.2 95,668 + -0.2 95,668 + +0.6 95,668 + +0.6 95,668 + -1.2 95,669 - -0.7 95,676 + -0.2 95,676 + -0.6 95,676 + -0.7 95,676 + +1.0 95,676 + +0.3 95,676 + -1.9 95,677 - -0.0 95,677 - +0.7 95,677 - -1.0 95,677 - +0.0 95,677 - +0.8 95,677 - -0.8 95,677 - +0.4 95,677 - +0.4 95,677 - +0.2 95,677 - +0.1 95,916 + GFF6190 0.15 +2.7 95,916 + GFF6190 0.15 +2.8 95,916 + GFF6190 0.15 +2.3 95,916 + GFF6190 0.15 +2.4 95,916 + GFF6190 0.15 +3.8 95,916 + GFF6190 0.15 +2.8 95,916 + GFF6190 0.15 +2.6 95,917 - GFF6190 0.15 +2.9 95,917 - GFF6190 0.15 +2.6 95,917 - GFF6190 0.15 +3.2 95,917 - GFF6190 0.15 +2.8 95,917 - GFF6190 0.15 +1.6 95,917 - GFF6190 0.15 +3.4 95,917 - GFF6190 0.15 +2.7 95,917 - GFF6190 0.15 +3.1 95,919 + GFF6190 0.15 +4.0 95,919 + GFF6190 0.15 +3.3 95,920 - GFF6190 0.15 +2.7 95,920 - GFF6190 0.15 +2.7 95,920 - GFF6190 0.15 +2.8
Or see this region's nucleotide sequence